Characterization of avian pathogenic Escherichia coli isolated in eastern China

文献类型: 外文期刊

第一作者: Dou, Xinhong

作者: Dou, Xinhong;Gong, Jiansen;Xu, Ming;Shen, Haiyu;Zhang, Di;Liu, Jiasheng;Zou, Jianmin;Dou, Xinhong;Gong, Jiansen;Shen, Haiyu;Zou, Jianmin;Han, Xiangan;Zhuang, Linlin

作者机构:

关键词: Avian pathogenic Escherichia coli;O-serogroup;Virulence-associated genes;Class I integrin;Drug resistance;Biofilm formation

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

ISSN: 0378-1119

年卷期: 2016 年 576 卷 1 期

页码:

收录情况: SCI

摘要: In order to investigate the biological characteristics of avian pathogenic Escherichia coli (APEC) isolated in eastern China, a total of 243 isolates were isolated from diseased poultry on different farms during the period from 2007 to 2014. These isolates were characterized for serogroups (polymerase chain reaction and agglutination), the presence of virulence -associated genes (fmC, iss, ompA, fyuA, stx2f, iroC, iucD, hlyE, tsh, cvaC, irp2, and papC) and class I integrons (polymerase chain reaction), drug susceptibilities (disk diffusion method) and the biofilm-forming abilities (semi -quantitative method). The results showed that the most predominant serogroups were O78 (87 isolates, 35.8%) and O2 (35 isolates, 14.4%). Gene profiling found thatfimC and ompA were frequently distributed among the isolates and that 77.4% of the isolates were positive for class 1 integrons. Overall, isolates displayed resistance to tetracycline (97.5%), nalidixic acid (82.3%), ampicillin (81.1%), sulphafurazole (80.7%), streptomycin (79.0%), trimethoprim (78.2%) and cotrimoxazole (782%). Multiple -drug resistance was exhibited in 80.3% of the isolates, and the presence of class 1 integrons is associated with multidrug resistance. Finally, 151 isolates had the ability to form biofilms in vitro, and drug resistance seemed relative to biofilmforming abilities. (C) 2015 Elsevier B.V. All rights reserved.

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