RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis

文献类型： 外文期刊

第一作者： Xu, Yue

作者： Xu, Yue;Zheng, Wen-Bin;Li, Hao-Yu;Cai, Lang;Xie, Shi-Chen;Zhu, Xing-Quan;Zou, Yang;Zhu, Xing-Quan;Elsheikha, Hany M.

作者机构：

关键词： Toxocara canis; lncRNA; mRNA; Transcriptomics; Beagle dog; Spleen

期刊名称：PARASITES & VECTORS （ 影响因子：4.047； 五年影响因子：4.223 ）

ISSN： 1756-3305

年卷期： 2022 年 15 卷 1 期

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收录情况： SCI

摘要： Background: Toxocara canis is a cosmopolitan parasite with a significant adverse impact on the health of humans and animals. The spleen is a major immune organ that plays essential roles in protecting the host against various infections. However, its role in T. canis infection has not received much attention. Methods: We performed sequencing-based transcriptome profiling of long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression in the spleen of Beagle puppies at 24 h post-infection (hpi), 96 hpi and 36 days post-infection (dpi). Deep sequencing of RNAs isolated from the spleen of six puppies (three infected and three control) at each time point after infection was conducted. Results: Our analysis revealed 614 differentially expressed (DE) lncRNAs and 262 DEmRNAs at 24 hpi; 726 DElncRNAs and 878 DEmRNAs at 96 hpi; and 686 DElncRNAs and 504 DEmRNAs at 36 dpi. Of those, 35 DElncRNA transcripts and 11 DEmRNAs were detected at all three time points post-infection. Many DE genes were enriched in immune response, such as ifit1, ifit2 and rorc. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that some genes (e.g. prkx and tnfrsf11a) were involved in the T cell receptor signaling pathway, calcium signaling pathway, Ras signaling pathway and NF-kappa B signaling pathway. Conclusions: The findings of this study show marked alterations in the expression profiles of spleen lncRNAs and mRNAs, with possible implications in the pathophysiology of toxocariasis.

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