RNAi-mediated transgenic rice resistance to Rice stripe virus

文献类型： 外文期刊

第一作者： Wang Biao

作者： Wang Biao;Lei Yang;Dai Yu-hua;He Wen;Liang Chun;Wang Xi-feng;Guo Cheng;Liang Chun;Zhou Tong;Liang Chun

作者机构：

关键词： Rice stripe virus (RSV);transgenic rice;deep sequencing;siRNA;resistance

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 11 期

页码：

收录情况： SCI

摘要： Rice stripe virus (RSV) often causes severe rice yield loss in temperate regions of East Asia. Although the correlation of small interfering RNAs (siRNAs) with transgenic virus resistance of plants using RNA interference (RNAi) is known for decades, no systematical research has been done on the profiling of siRNAs from a genomic scale. Our research is aiming to systematically study the RNAi impact in RSV-resistant transgenic rice, which was generated by introducing an inverted repeat construct that targets RSV nucleocapsid protein (NCP) gene. In this paper, three independent RSV-resistant transgenic rice lines were generated, their stable integration of the T-DNA fragment and the expression of siRNAs were confirmed by Southern blotting and Northern blotting analyses, and the majority of siRNAs were in lengths of 21, 22, and 24 nucleotides (nt), which have validated a connection between the presence of the RSV NCP homologous siRNAs and the RSV resistance in those transgenic rice lines. In one of these transgenic lines (T4-B1), the T-DNA fragment was found to have been inserted at chromosome 1 of the rice genome, substituting the rice genome fragment from 32 158 773 to 32 158 787 nt. Bioinformatics analysis of small RNA-Seq data on the T4-B1 line also confirmed the large population of NCP-derived siRNAs in transgenic plants, and the RSV-infected library (T4-B1-V) possessed more siRNAs than its mock inoculated libraries (T4-B1-VF), these results indicating the inverted repeat construct and RSV could introduce abundance of siRNAs in transgenic rice. Moreover, a varied expression level of specific siRNAs was found among different segments of the NCP gene template, about 47% of NCP-derived siRNAs reads aligned with the fragment from 594 to 832 nt (239 nt in length) in NCP gene (969 nt in length) in the T4-B1-V, indicating a potential usage of hotspot regions for RNAi silencing in future research. In conclusion, as the first study to address the siRNA profile in RSV-resistant transgenic plant using next generation sequencing (NGS) technique, we confirmed that the massive abundance of siRNA derived from the inverted repeat of NCP is the major reason for RSV-resistance.

分类号：

  • 相关文献

[1]Baseline susceptibility of Cnaphalocrocis medinalis (Lepidoptera : Pyralidae) to Bacillus thuringiensis toxins in China. Hou, M. L.,Peng, Y. F.,Liu, P. L..

[2]Identification and regulation of host genes related to Rice stripe virus symptom production. Shi, Bingbin,Zhou, Hong,Jiang, Tong,Shi, Bingbin,Lin, Lin,Wang, Shihui,Rong, Lingling,Li, Junmin,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Yang, Yong,Zhao, Jinping,Chen, Jianping,Yan, Fei,Guo, Qin,Chen, Zhuo,Song, Baoan.

[3]A one-step real time RT-PCR assay for quantifying rice stripe virus in rice and in the small brown planthopper (Laodelphax striatellus fallen). Wang, Xifeng,Zhou, Guanghe. 2008

[4]Novel loci for field resistance to black-streaked dwarf and stripe viruses identified in a set of reciprocal introgression lines of rice (Oryza sativa L.). Zhai, Huqu,Zhu, Linghua,Uzokwe, Veronica N. E.,Xu, Jianlong,Li, Zhikang,Yang, Jie,Zhong, Weigong,Fan, Fangjun,Yang, Jinhuan,Wang, Jun,Zhu, Jinyan,Ali, A. Jauhar,Li, Zhikang. 2012

[5]Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in bread wheat (Triticum aestivum L.) and Brachypodium distachyon (L.) Beauv. Wei, Bo,Zhang, Rongzhi,Li, Aili,Jia, Jizeng,Mao, Long,Wei, Bo,Zhang, Rongzhi,Li, Aili,Jia, Jizeng,Mao, Long,Cai, Tao,Li, Shan,Qi, Yijun,Huo, Naxin,Gu, Yong Q.,Vogel, John.

[6]Co-silence of the coatomer beta and v-ATPase A genes by siRNA feeding reduces larval survival rate and weight gain of cotton bollworm, Helicoverpa armigera. Zeng, Fanrong,Zhang, Peizhuo,Liu, Changyan.

[7]Cross-inhibition to heterologous foot-and-mouth disease virus infection induced by RNA interference targeting the conserved regions of viral genome. Liu, MQ,Chen, WZ,Ni, Z,Yan, WY,Fei, L,Jiao, Y,Zhang, J,Du, QY,Wei, X,Chen, JL,Liu, YM,Zheng, ZX.

[8]Peroxiredoxin IV Regulates Pro-Inflammatory Responses in Large Yellow Croaker (Pseudosciaena crocea) and Protects against Bacterial Challenge. Yu, Suhong,Mu, Yinnan,Ao, Jingqun,Chen, Xinhua,Yu, Suhong.

[9]Analysis of transcriptional and epigenetic changes in hybrid vigor of allopolyploid Brassica napus uncovers key roles for small RNAs. Shen, Yifei,Sun, Shuo,Shen, Enhui,Ye, Chu-Yu,Fan, Longjiang,Shen, Yifei,Sun, Shuo,Shen, Enhui,Ye, Chu-Yu,Fan, Longjiang,Hua, Shuijin,Cai, Daguang,Timko, Michael P.,Zhu, Qian-Hao.

[10]Galactosylated poly(ethylene glycol) methacrylate-st-3-guanidinopropyl methacrylamide copolymers as siRNA carriers for inhibiting Survivin expression in vitro and in vivo. Wu, Yang,Qin, Zhu,Ji, Jinkai,Yang, Ran,Li, Xingsong,Qin, Zhu,Zhang, Xiaoqiang,Li, Yuanhui,Yin, Lihong,Pu, Yuepu.

[11]Expression pattern of L-FABP gene in different tissues and its regulation of fat metabolism-related genes in duck. He, Jun,Tian, Yong,Li, Jinjun,Shen, Junda,Tao, Zhengrong,Lu, Lizhi,He, Jun,Fu, Yan,Niu, Dong.

[12]gga-miR-9*inhibits IFN production in antiviral innate immunity by targeting interferon regulatory factor 2 to promote IBDV replication. Ouyang, Wei,Liu, Hua-jie,Zhang, Hai-bin,Ouyang, Wei,Wang, Yong-shan,Du, Xi-ning,Liu, Hua-jie,Ouyang, Wei,Wang, Yong-shan.

[13]Identification of Strawberry vein banding virus encoded P6 as an RNA silencing suppressor. Feng, Mingfeng,Zuo, Dengpan,Jiang, Xizi,Li, Shuai,Chen, Jing,Jiang, Lei,Jiang, Tong,Zhou, Xueping. 2018

[14]Prediction of VIGS efficiency by the Sfold program and its reliability analysis in Gossypium hirsutum. Xiaoyang Ge,Jie Wu,Chaojun Zhang,Qianhua Wang,Yuxia Hou,Zuoren Yang,Zhaoen Yang,Zhenzhen Xu,Ye Wang,Lili Lu,Xueyan Zhang,Jinping Hua,Fuguang Li. 2016

[15]Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells. Zhang, H.,Chen, F.,Tao, Z. R.,Li, J. J.,Lu, L. Z.,Zhang, H.,Chen, F.,Li, G. L.,Ding, Y. Y.,Zhong, S. L.. 2014

[16]Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum. Fan Jia,Sun Jing-rui,Chen Ju-lian. 2015

[17]Staphylococcus aureus infection of intestinal epithelial cells induces human umbilical cord-derived mesenchymal stem cell migration. Li, Yang,Liu, Ming-yuan,Wang, Xue-lin,Han, Wen-yu,Liu, Ya-hui,Suo, Jian,Li, Zhi-jie,Li, Ya-gang,Jin, Hu. 2013

[18]Study on the RNA interference of Sry in mouse embryos. Zhao Jin-Hong,Du Wei-Hua,Dao Er-Ji,Pei Jie,Zhang Li,Lin Xiu-Kun. 2006

[19]Interference of porcine reproductive and respiratory syndrome virus replication on MARC-145 cells using DNA-based short interfering RNAs. He, Yun-xia,Hua, Rong-hong,Zhou, Yan-jun,Qiu, Hua-ji,Tong, Guang-zhi. 2007

[20]Expression of IL-13R alpha 2 in liver cancer cells and its effect on targeted therapy of liver cancer. Hou, Lingling,Du, Juan,Liu, Yanfeng,Sun, Weimin,Zheng, Yanpeng,Zhang, Lishu,Hu, Honggang,Dai, Xinxian,Hong, Tao,Wang, Jianwei,Guan, Weijun,Ma, Yuehui. 2010

作者其他论文 更多>>

微信小程序