Muscle hypertrophy in transgenic mice due to over-expression of porcine myostatin mutated at its cleavage site

文献类型： 外文期刊

第一作者： Qian Li-li

作者： Qian Li-li;Cai Chun-bo;An Xiao-rong;Ma De-zun;Gao Peng-fei;Jiang Sheng-wang;Wang Qing-qing;Xiao Gao-jun;Cui Wen-tao

作者机构：

关键词： porcine myostatin;muscle mass;transgenic mice;mutation;hypertrophy;hyperplasia

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 11 期

页码：

收录情况： SCI

摘要： Myostatin, a member of the transforming growth factor beta (TGF-beta) superfamily, is a dominant inhibitor that acts to limit skeletal muscle growth and development. In this study, we generated transgenic mice that express porcine myostatin containg mutations at its cleavage site (RSRR) to evaluate its effect on muscle mass. Results showed that the weight of four skeletal muscles including gastrocnemius, rectus femoris, tibialis anterior, and pectoralis increased by 17.83 and 28.39%, 21.76 and 28.70%, 34.31 and 41.62%, 53.21 and 27.54% in transgenic male and female mice, respectively, compared to their corresponding non-transgenic control mice. Measurement of muscle fiber size and number indicated that the mean myofiber size increased by 50.73 and 61.30% in transgenic male and female mice respectively compared to the non-transgenic controls. However, there was no difference in the number of myofiber between transgenic and non-transgenic male mice. These results clearly demonstrated that the increase in skeletal muscle mass in transgenic mice is caused by hypertrophy instead of hyperplasia.

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