Engineering broad-spectrum disease-resistant rice by editing multiple susceptibility genes

文献类型： 外文期刊

第一作者： Tao, Hui

作者： Tao, Hui;Wang, Dan;Fang, Hong;Liu, Xionglun;Tao, Hui;Shi, Xuetao;He, Feng;Fang, Hong;Wang, Ruyi;Zhang, Fan;Wang, Min;Ning, Yuese;Wang, Guo-Liang;Xiao, Ning;Li, Aihong

作者机构：

关键词： broad-spectrum resistance; CRISPR; Cas9; gene editing; Magnaporthe oryzae; susceptibility gene; Xanthomonas oryzae pv; oryzae

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2021 年 63 卷 9 期

页码：

收录情况： SCI

摘要： Rice blast and bacterial blight are important diseases of rice (Oryza sativa) caused by the fungus Magnaporthe oryzae and the bacterium Xanthomonas oryzae pv. oryzae (Xoo), respectively. Breeding rice varieties for broad-spectrum resistance is considered the most effective and sustainable approach to controlling both diseases. Although dominant resistance genes have been extensively used in rice breeding and production, generating disease-resistant varieties by altering susceptibility (S) genes that facilitate pathogen compatibility remains unexplored. Here, using CRISPR/Cas9 technology, we generated loss-of-function mutants of the S genes Pi21 and Bsr-d1 and showed that they had increased resistance to M. oryzae. We also generated a knockout mutant of the S gene Xa5 that showed increased resistance to Xoo. Remarkably, a triple mutant of all three S genes had significantly enhanced resistance to both M. oryzae and Xoo. Moreover, the triple mutant was comparable to the wild type in regard to key agronomic traits, including plant height, effective panicle number per plant, grain number per panicle, seed setting rate, and thousand-grain weight. These results demonstrate that the simultaneous editing of multiple S genes is a powerful strategy for generating new rice varieties with broad-spectrum resistance.

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