Rumen fermentation and bacterial communities in weaned Chahaer lambs on diets with different protein levels

文献类型： 外文期刊

第一作者： Yang Chun-tao

作者： Yang Chun-tao;Si Bing-wen;Diao Qi-yu;Zeng Shu-qin;Tu Yan;Jin Hai

作者机构：

关键词： dietary crude protein;rumen fermentation;bacterial diversity;weaned Iamb;PCR-DGGE

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 7 期

页码：

收录情况： SCI

摘要： We evaluated the effects of diets with different crude protein (CP) levels on growth performance, rumen fermentation and bacterial communities in weaned Chahaer lambs. 128 weaned Chahaer lambs ((20.56 +/- 1.43) kg body weight; ram:ewe 1:1) aged (61 +/- 1.85) d were randomly allotted to one of four diets with CP content of 11.17% (T1), 12.06% (T2), 13.40% (T3) or 14.36% (T4). Ruminal fermentation parameters were measured and bacterial communities were analysed using PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative PCR. The average daily gain and feed utilization efficiency in T3 were higher than those in the other groups (P<0.05), although the dry matter intake and metabolizable energy intake were similar. Total volatile fatty acid concentration in the ruminal fluid of T3 was lower than that of T1 (P=0.011), T2 (P=0.008) or T4 (P=0.309). The ammonia nitrogen concentration and acetate/propionate ratio of ruminal fluid were significantly higher in lambs fed the higher CP diets, whereas the molar concentrations of propionate and butyrate of ruminal fluid were lower. The rumen bacterial community was similar in T2 and T3 which shown more stable and diverse rumen microbes ecosystem compared with the other groups. The DGGE profiles and phylogenetic tree indicated that Bacteroides uniformis, Clostridium alkalicellulosi, Alkalibaculum bacchi and Saccharofermentans sp. were common bacterium of Chahaer lamb rumen. B. uniformis, C. alkalicellulosi, Saccharofermentans sp. and Gracilibacter thermotolerans, which belong to the Bacteroidetes and Firmicutes phyla, were the dominant species in the rumen of lambs fed 13.40% CP. However, Ruminococcus albus, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens were not different in lambs fed different CP diets. Therefore, it could be concluded that B. uniformis, C. alkalicellulosi, A. bacchi and Saccharofermentans sp. were common bacteria of Chahaer lamb rumen. Furthermore, the dietary CP of 13.04% could improve performance and change rumen fermentation model by increasing the dominant species' peak intensities of B. uniformis, C. alkalicellulosi, Saccharofermentans sp. and Gracilibacter thermotolerans and stabilizing rumen microbial ecosystem.

分类号：

  • 相关文献

[1]Effect of methionine source and dietary crude protein level on growth performance, carcass traits and nutrient retention in chinese color-feathered chicks. Xi, P. B.,Yi, C. F.,Lin, Y. C.,Zheng, C. T.,Jiang, Z. Y.,Vazquez-Afion, M.,Song, C. L.,Knight, C. D.. 2007

[2]Effects of Substituting Beer Lees and Cassava Residues Respectively for Buffalo Dietary Soybean Meal and Grassiness on Rumen Fermentation In Vitro. Xia, Zhongsheng,Huang, Yali,Zou, Caixia,Chen, Zhihui,Liang, Xianwei,Wei, Shengju,Liang, Xin,Li, Shulu,Huang, Yali. 2013

[3]Effect of zinc source on performance, zinc status, immune response, and rumen fermentation of lactating cows. Wang, Run L.,Liang, Jian G.,Lu, Lin,Zhang, Li Y.,Li, Su F.,Luo, Xu G.,Wang, Run L.,Li, Su F..

[4]Effects of selenium yeast on rumen fermentation, lactation performance and feed digestibilities in lactating dairy cows. Wang, C.,Liu, Q.,Yang, W. Z.,Dong, Q.,Dong, K. H.,Huang, Y. X.,Yang, W. Z.,Yang, X. M.,He, D. C.,Zhang, P..

[5]Soybean oil and linseed oil supplementation affect profiles of ruminal microorganisms in dairy cows. Yang, S. L.,Bu, D. P.,Wang, J. Q.,Hu, Z. Y.,Li, D.,Wei, H. Y.,Zhou, L. Y.,Loor, J. J..

[6]Feeding different dietary protein to energy ratios to Holstein heifers: effects on growth performance, blood metabolites and rumen fermentation parameters. Dong, L. F.,Zhang, W. B.,Zhang, N. F.,Tu, Y.,Diao, Q. Y..

[7]Identification of the adherent microbiota on the gills and skin of poly-cultured gibel carp (Carassius auratus gibelio) and bluntnose black bream (Megalobrama amblycephala Yih). Wang, Wenwen,Zhou, Zhigang,He, Suxu,Liu, Yuchun,Cao, Yanan,Shi, Pengjun,Yao, Bin,Ringo, Einar.

[8]Oral administration of Lactobacillus plantarum and Bacillus subtilis on rumen fermentation and the bacterial community in calves. Zhang, Rong,Dong, Xiaoli,Zhou, Meng,Tu, Yan,Zhang, Naifeng,Diao, Qiyu,Deng, Kaidong.

[9]The biogeography of fungal communities in wetland sediments along the Changjiang River and other sites in China. Tian, Jianqing,Xiang, Meichun,Sun, Jingzu,Liu, Xingzhong,Wu, Bing,Bai, Chunming.

[10]Effects of essential oil combinations on sheep ruminal fermentation and digestibility of a diet with fumarate included. Lin, B.,Lu, Y.,Wang, J. H.,Liang, Q.,Liu, J. X.,Lin, B.,Salem, A. Z. M.,Salem, A. Z. M..

[11]Improved composting of poultry feces via supplementation with ammonia oxidizing archaea. Xie, Kaizhi,Xu, Peizhi,Huang, Xu,Gu, Wenjie,Zhang, Fabao,Yang, Shaohai,Tang, Shuanhu,Xie, Kaizhi,Jia, Xiaoshan.

[12]Effects of physico-chemical parameters on the bacterial and fungal communities during agricultural waste composting. Zhang, Jiachao,Zeng, Guangming,Chen, Yaoning,Yu, Zhen,Li, Hui,Yu, Yong,Zhang, Jiachao,Zeng, Guangming,Chen, Yaoning,Yu, Zhen,Li, Hui,Yu, Yong,Yu, Man,Huang, Hongli.

[13]Effects of pretreatment on the microbial community and L-lactic acid production in vinasse fermentation. Liu, Jianguo,Wang, Qunhui,Wang, Shuang,Ma, Hongzhi,Liu, Jianguo,Sun, Xiaohong,Tushiro, Yukihiro.

[14]Effects of citric acid supplementation on rumen fermentation, urinary excretion of purine derivatives and feed digestibility in steers. Liu, Qiang,Yang, Wen Zhu,Wang, Cong,Meng, Jie,Yang, Wen Zhu,Dong, Kuan Hu,Huang, Ying Xiang,Yang, Xiao Min,He, Dong Chang.

[15]Effect of high-dose nano-selenium and selenium-yeast on feed digestibility, rumen fermentation, and purine derivatives in sheep. Xun, Wenjuan,Yue, Wenbin,Zhang, Chunxiang,Ren, Youshe,Liu, Qiang,Xun, Wenjuan,Shi, Liguang.

[16]Potted rice straw increased the antioxidase activity and growth of Chinese green cabbage seedlings and altered the soil microbial community. Wu, Hong-Sheng,Wang, Ming-Yan,Shi, Xue,Ren, Qian-Qi,Li, Ji,Chen, Su-yun,Xu, Ya,Huang, Hong-Ying,Liu, Ya-Dong,Zhou, Ji.

[17]Biodiversity and seasonal variation of the cyanobacterial assemblage in a rice paddy field in Fujian, China. Song, TY,Martensson, L,Eriksson, T,Zheng, WW,Rasmussen, U.

[18]Effects of isobutyrate on rumen fermentation, lactation performance and plasma characteristics in dairy cows. Liu, Q.,Wang, C.,Yang, W. Z.,Zhang, B.,Dong, K. H.,Huang, Y. X.,Yang, W. Z.,Yang, X. M.,He, D. C.,Zhang, P.. 2009

[19]Community composition of ammonia-oxidizing bacteria in the rhizosphere of intercropped wheat (Triticum aestivum L.), maize (Zea mays L.), and faba bean (Vicia faba L.). Song, Y. N.,Marschner, P.,Li, L.,Bao, X. G.,Sun, J. H.,Zhang, F. S.. 2007

[20]Bacterial diversity of gut content in sea cucumber (Apostichopus japonicus) and its habitat surface sediment. Gao Fei,Tan Jie,Sun Huiling,Yan Jingping. 2014

作者其他论文 更多>>

微信小程序