Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/beta-Catenin Signaling

文献类型： 外文期刊

第一作者： Zhang, Haihua

作者： Zhang, Haihua;Wang, Shiyong;Zhang, Tietao;Yang, Fuhe;Li, Guangyu;Nan, Weixiao;Si, Huazhe

作者机构：

关键词： Epidermal growth factor;Outer root sheath;Proliferation;Migration;Wnt/beta-catenin signaling

期刊名称：CELLULAR PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.5； 五年影响因子：4.354 ）

ISSN： 1015-8987

年卷期： 2016 年 39 卷 1 期

页码：

收录情况： SCI

摘要： Background/Aims: To investigate the effect and molecular mechanism of EGF on the growth and migration of hair follicle outer root sheath (ORS) cells. Methods: Intact anagen hair follicles were isolated from mink skin and cultured with EGF in vitro to measure ORS daily growth. Meanwhile, purified primary ORS cells were treated or transfected with EGF, and their proliferation and migration were assessed by MTT assay and transwell assay, respectively. The signaling pathway downstream of EGF was characterized by using the Wnt/beta-catenin signaling inhibitor, XAV-939. Results: EGF of 2-20 ng/ml, not higher or lower, promoted the growth of follicular ORS in vitro. EGF treatment or overexpression promoted the proliferation and migration of ORS cells. Moreover, EGF stimulation induced nuclear translocation of beta-catenin, and upregulated the expression of Wnt10b, beta-catenin, EGF receptor and SOX9. Inhibition of Wnt/beta-catenin signaling by XAV-939 significantly reduced the basal and EGF-enhanced proliferation and migration of ORS cells. In addition, a number of follicle-regulatory genes, such as Survivin, Msx2 and SGK3, were upregulated by EGF in the ORS cells, which was also inhibited by XAV-939. Conclusion: EGF promotes the proliferation and migration of ORS cells and modulates the expression of several follicle-regulatory genes via Wnt/beta-catenin signaling. (C) 2016 The Author(s) Published by S. Karger AG, Basel

分类号：

  • 相关文献

[1]Up-regulation of miR-182-5p predicts poor prognosis in patients with lung cancer and associates with tumor cell growth and migration. Shi, Yuanyuan,Liu, Hao,Du, Xiang,Liu, Xuedong,Liu, Jing. 2017

[2]FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells. Li, Shuying,Jiang, Zhonghao,Xu, Wenyi,Xie, Yingying,Zhao, Leiming,Tang, Xuanming,Wang, Fengzhong,Xin, Fengjiao.

[3]Characterization of a new fungal immunomodulatory protein, FIP-dsq2 from Dichomitus squalens. Li, Shuying,Jiang, Zhonghao,Sun, Lichao,Liu, Xin,Huang, Ying,Wang, Fengzhong,Xin, Fengjiao.

[4]Knockdown of HIF-1 alpha inhibits the proliferation and migration of outer root sheath cells exposed to hypoxia in vitro: An involvement of Shh pathway. Zhang, Haihua,Song, Xingchao,Wang, Shiyong,Si, Huazhe,Li, Guangyu,Nan, Weixiao. 2017

[5]Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway. Zhang, Haihua,Wang, Shiyong,Zhang, Tietao,Wang, Datao,Yang, Fuhe,Li, Guangyu,Nan, Weixiao,Si, Huazhe. 2016

[6]Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice. Han, Jie,Xu, Yunhe,Yang, Di,Yu, Ning,Bai, Zishan,Bian, Lianquan,Xu, Yunhe,Yu, Ning.

[7]Antimony release from contaminated mine soils and its migration in four typical soils using lysimeter experiments. Zhao, Long,Hou, Hong,Shangguan, Yu-xian,Qin, Yusheng,Zhang, Naiming.

[8]Molecular cloning, sequence analysis, and function of the intestinal epithelial stem cell marker Bmi1 in pig intestinal epithelial cells. Li, C. -M.,Yan, H. -C.,Fu, H. -L.,Xu, G. -F.,Wang, X. -Q.. 2014

[9]The Influence of Thidiazuron on Proliferation of Acer x freemanii In Vitro. Zhao, X. Q.,Yuan, Z. H.,Yin, Y. L.,Feng, L. J.,Yin, Y. L.,Xu, R.. 2012

[10]Effect of Auxins on In Vitro Propagation of Sour Jujube Infected by Witches' Broom Phytoplasma. Sun, Q.,Zhou, G.,Sun, H.,Giang, G.. 2009

[11]Effects of linoleic acid and eicosapentaenoic acid on cell proliferation and lipid-metabolism gene expression in primary duck hepatocytes. Liu, W. M.,Lu, L. Z.,Zhang, C.,Liu, Y. L.,Zhang, J.,Tao, Z. R.,Shen, J. D.,Li, G. Q.,Wang, D. Q.,Li, J. J.,Tian, Y.,Liu, W. M.,Shi, F. X.,Liu, Y. L.,Zhang, J.. 2011

[12]Relationship of flight and reproduction in beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), a migrant lacking the oogenesis-flight syndrome. Jiang, Xing Fu,Luo, Li Zhi,Sappington, Thomas W.. 2010

[13]Spring migration and summer dispersal of Loxostege sticticalis (Lepidoptera : Pyralidae) and other insects observed with radar in Northern China. Wu, KM,Cheng, DF,Guo, YY. 2004

[14]Variation of pre-ovipositional period in the brown planthopper, Nilaparvata lugens, collected in tropical, subtropical and temperate Asia. Wada, T.,Ito, K.,Takahashi, A.,Tang, J.. 2007

[15]The anti-inflammatory effect of the SOCC blocker SK&F 96365 on mouse lymphocytes after stimulation by Con A or PMA/ionomycin. Ye, Yanxia,Zhang, Yaxing,Lu, Xiaoyu,Huang, Xiuyan,Zeng, Xiangfeng,Zeng, Yaoying,Lu, Xiaoyu,Lai, Xinqiang. 2011

[16]Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Sun, Yingning. 2013

[17]Do second generation Asia corn borer (Lepidoptera: Crambidae) immigrate to corn fields from alternate habitats?. Chen, Ri-Zhao,Li, Lian-Bing,Li, Peng-Pei,Klein, Michael G.,Li, Qi-Yun,Sheng, Cheng-Fa. 2015

[18]Different genetic structures revealed resident populations of a specialist parasitoid wasp in contrast to its migratory host. Wei, Shu-Jun,Zhou, Yuan,Fan, Xu-Lei,Cao, Li-Jun,Zhou, Yuan,Xu, Zai-Fu,Hoffmann, Ary A.,Chen, Xue-Xin. 2017

[19]Migration and population structure characteristics of the small yellow croaker Larimichthys polyactis in the southern Yellow Sea. Xiong Ying,Yang Jian,Xiong Ying,Zhong Xiaming,Tang Jianhua,Yang Jian. 2016

[20]Integrated Analyses Reveal Overexpressed Notch1 Promoting Porcine Satellite Cells' Proliferation through Regulating the Cell Cycle. Jiao, Yiren,Huang, Bo,Chen, Yu,Hong, Guangliang,Xu, Jian,Wang, Chong,Hu, Chingyuan. 2018

作者其他论文 更多>>

微信小程序