Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins

文献类型： 外文期刊

第一作者： Li, Peiwu

作者： Li, Peiwu;Zhou, Qian;Wang, Ting;Zhou, Haiyan;Zhang, Wen;Ding, Xiaoxia;Zhang, Zhaowei;Zhang, Qi;Li, Peiwu;Zhou, Haiyan;Zhang, Wen;Ding, Xiaoxia;Zhang, Zhaowei;Zhang, Qi;Li, Peiwu;Zhou, Haiyan;Zhang, Wen;Ding, Xiaoxia;Zhang, Zhaowei;Zhang, Qi;Li, Peiwu;Zhou, Haiyan;Zhang, Wen;Ding, Xiaoxia;Zhang, Zhaowei;Zhang, Qi;Zhou, Qian;Chang, Perng-Kuang

作者机构：

关键词： aflatoxin G(1);class-specific;monoclonal antibody;ELISA;Aspergillus flavus

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2016 年 8 卷 1 期

页码：

收录情况： SCI

摘要： To detect and monitor G-group aflatoxins in agricultural products, we generated class-specific monoclonal antibodies that specifically recognized aflatoxins G(1) and G(2). Of the final three positive and stable hybridomas obtained, clone 2G6 produced a monoclonal antibody that had equal sensitivity to aflatoxins G(1) and G(2), and did not cross-react with aflatoxins B-1, B-2, or M-1. Its IC50 values for aflatoxins G(1) and G(2) were 17.18 ng.mL(-1) and 19.75 ng.mL(-1), respectively. Using this new monoclonal antibody, we developed a competitive indirect enzyme-linked immunosorbent assay (CI-ELISA); the method had a limit of detection of 0.06 ng.mL(-1). To validate this CI-ELISA, we spiked uncontaminated peanut samples with various amounts of aflatoxins G(1) and G(2) and compared recovery rates with those determined by a standard HPLC method. The recovery rates of the CI-ELISA ranging from 94% to 103% were comparable to those of the HPLC (92% to 102%). We also used both methods to determine the amounts of G-group aflatoxins in five peanut samples contaminated by aflatoxin B-1-positive, and their relative standard deviations ranged from 8.4% to 17.7% (under 20%), which demonstrates a good correlation between the two methods. We further used this CI-ELISA to assess the ability of 126 fungal strains isolated from peanuts or field soils to produce G-group aflatoxins. Among these, seven stains producing different amounts of G-group aflatoxins were identified. Our results showed that the monoclonal antibody 2 G6-based CI-ELISA was suitable for the detection of G-group aflatoxins present in peanuts and also those produced by fungi.

分类号：

  • 相关文献

[1]Development Of A Class-specific Monoclonal Antibody-based Elisa For Aflatoxins In Peanut. Li, Peiwu,Zhang, Qi,Zhang, Wen,Zhang, Jinyang,Chen, Xiaomei,Jiang, Jun,Xie, Lihua,Zhang, Daohong.

[2]Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene. Qiao, Bin,Li, Yansong,Meng, Xingyu,Sun, Yu,Hu, Pan,Lu, Shiying,Ren, Honglin,Liu, Zengshan,Zhou, Yu,Meng, Xingyu,Sun, Yu,Zhou, Yu.

[3]A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for 5-Formyltetrahydrofolate Detection in Maize Kernels. Yue, Huanfang,Zhang, Wei,Cao, Zhen,Tan, Guiyu,Wang, Baomin,Yue, Huanfang,Liang, Qiuju,Zhang, Chunyi.

[4]Production and characterization of monoclonal antibody for class-specific determination of O,O-dimethyl organophosphorus pesticides and effect of heterologous coating antigens on immunoassay sensitivity. Liu, Yan,Lou, Yang,Xu, Dan,Qian, Guoliang,Wu, Runru,Hu, Baishi,Liu, Fengquan,Zhang, Qi.

[5]Preparation and Identification of a Monoclonal Antibody Specific to a Broad Spectrum of O, O-diethyl Organophosphorus Pesticides. Zhang Li-Jie,Pan Jia-Rong. 2010

[6]Generation and Application of Monoclonal Antibodies Specific for the Envelope Protein pORF132 of Cyprinid Herpesvirus 3. Liu, Zhenxing,Hao, Le,Ma, Yanping,Liang, Zhiling,Ma, Jiangyao,Ke, Hao.

[7]Preparation of Monoclonal Antibody for Melamine and Development of an Indirect Competitive ELISA for Melamine Detection in Raw Milk, Milk Powder, and Animal Feeds. Zhang, Taichang,Meng, Meng,Xi, Rimo,Yin, Weiwei,Liu, Jinting,Li, Weihua,Liu, Wei,He, Fangyang,Wan, Yuping,Feng, Caiwei,Wang, Shanliang,Lu, Xiao.

[8]Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA to detect diethylstilbestrol in biological samples. Li WeiHua,Liu Wei,Yin WeiWei,Xu Jing,Liu JinTing,Meng Meng,Xue HuYin,Xi RiMo,Meng Meng,Xue HuYin,Xi RiMo,He FangYang,Wan YuPing,Feng CaiWei,Wang ShanLiang,Lu Xiao. 2011

[9]Isolation and characterization of Aspergillus flavus strains in China. Mamo, Firew Tafesse,Wang, Yan,Liu, Yang,Mamo, Firew Tafesse,Wang, Yan,Liu, Yang,Shang, Bo,Selvaraj, Jonathan Nimal. 2018

[10]Optimization of Aflatoxin Production of Aspergillus Flavus on peanuts. Zhang Chushu,Sun Jie,Yu Lina,Yang Qingli,Feng Jianxiong. 2014

[11]Study on the key environmental factors Aspergillus flavus growth and aflatoxin production. Zhang Chushu,Sun Jie,Yu Lina,Bi Jie,Yang Qingli,Zhao Qin,Feng Jianxiong,Fu Xiaoji,Gao Yanan. 2014

[12]Gene Expression Profiling and Identification of Resistance Genes to Aspergillus flavus Infection in Peanut through EST and Microarray Strategies. Bhatnagar, Deepak,Yu, Jiujiang,Guo, Baozhu,Fedorova, Natalie D.,Wan, Chun-Hua,Wang, Wei,Nierman, William C.,Chen, Xiaoping,Chen, Xiaoping,Nierman, William C.. 2011

[13]A strain of Aspergillus flavus from China shows potential as a biocontrol agent for aflatoxin contamination. Zhou, Lu,Wei, Dan-Dan,Selvaraj, Jonathan Nimal,Shang, Bo,Zhang, Chu-Shu,Xing, Fu-Guo,Zhao, Yue-Ju,Wang, Yan,Liu, Yang.

[14]Cinnamaldehyde inhibits fungal growth and aflatoxin B-1 biosynthesis by modulating the oxidative stress response of Aspergillus flavus. Sun, Qi,Shang, Bo,Liu, Yang,Wang, Ling,Lu, Zhisong.

[15]Aflatoxin B-1 inhibition in Aspergillus flavus by Aspergillus niger through down-regulating expression of major biosynthetic genes and AFB(1) degradation by atoxigenic A. flavus. Xing, Fuguo,Wang, Limin,Liu, Xiao,Selvaraj, Jonathan Nimal,Wang, Yan,Zhao, Yueju,Liu, Yang.

[16]Development and evaluation of ITS- and aflP-based LAMP assays for rapid detection of Aspergillus flavus in food samples. Liu, Peiqing,Li, Benjin,Yin, Rongmei,Weng, Qiyong,Chen, Qinghe.

[17]Autoxidated linolenic acid inhibits aflatoxin biosynthesis in Aspergillus flavus via oxylipin species. Yan, Shijuan,Chen, Zhuang,Yan, Shijuan,Liang, Yating,Zhang, Jindan,Liu, Chun-Ming.

[18]Effect of water activity and temperature on the growth of Aspergillus flavus, the expression of aflatoxin biosynthetic genes and aflatoxin production in shelled peanuts. Liu, Xiao,Guan, Xuanli,Xing, Fuguo,Lv, Cong,Dai, Xiaofeng,Liu, Yang.

[19]The effectiveness of ISSR profiling for studying genetic diversity of Aspergillus flavus from peanut-cropped soils in China. Zhang, Chu-Shu,Xing, Fu-Guo,Selvaraj, Jonathan Nimal,Zhou, Lu,Zhao, Yue-Ju,Liu, Yang,Zhang, Chu-Shu,Yang, Qing-Li.

[20]Ethylene inhibited aflatoxin biosynthesis is due to oxidative stress alleviation and related to glutathione redox state changes in Aspergillus flavus. Liao, B. -S.. 2009

作者其他论文 更多>>

微信小程序