Preparative Separation of Main Ustilaginoidins from Rice False Smut Balls by High-Speed Counter-Current Chromatography

文献类型： 外文期刊

第一作者： Sun, Weibo

作者： Sun, Weibo;Dong, Xuejiao;Xu, Dan;Meng, Jiajia;Fu, Xiaoxiang;Wang, Xiaohan;Lai, Daowan;Zhou, Ligang;Liu, Yang

作者机构：

关键词： ustilaginoidins;mycotoxin;preparative separation;rice false smut balls;Villosiclava virens;Ustilaginoidea virens;high-speed counter-current chromatography

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2016 年 8 卷 1 期

页码：

收录情况： SCI

摘要： Ustilaginoidins are bis-naphtho-gamma-pyrone mycotoxins isolated from the rice false smut balls (FSBs) infected by the pathogen Villosiclava virens in rice spikelets on panicles. In order to obtain large amounts of pure ustilaginoidins to further evaluate their biological activities and functions, phytotoxicity on rice, security to human and animals as well as to accelerate their applications as pharmaceuticals, preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of seven bis-naphtho-gamma-pyrone mycotoxins, namely ustilaginoidins A (1), G (2), B (3), H (4), I (5), C (6), and J (7) from the ethyl acetate crude extract of rice FSBs. Both 1 and 2 were prepared by HSCCC from the low-polarity fraction of the crude extract using the two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at the volume ratio of 6.5:3.5:5.0:5.0. Similarly, 3, 4 and 5 were prepared from the medium-polarity fraction using the system at the volume ratio of 4.0:5.0:5.0:6.0, and 6 and 7 were prepared from the higher-polarity fraction using the system at volume ratio of 3.0:5.0:4.0:6.7. A total of 6.2 mg of 1, 5.1 mg of 2, 3.9 mg of 3, 1.2 mg of 4, 5.7 mg of 5, 3.5 mg of 6, and 6.1 mg of 7 with purities of 88%, 82%, 91%, 80%, 92%, 81% and 83%, respectively, were yielded from total 62 mg fraction samples in three independent HSCCC runs. The structures of the purified ustilaginoidins were characterized by means of physicochemical and spectrometric analysis.

分类号：

  • 相关文献

[1]Main Ustilaginoidins and Their Distribution in Rice False Smut Balls. Meng, Jiajia,Sun, Weibo,Mao, Ziling,Xu, Dan,Wang, Xiaohan,Lu, Shiqiong,Lai, Daowan,Zhou, Ligang,Zhang, Guozhen,Liu, Yang. 2015

[2]The Contents of Ustiloxins A and B along with Their Distribution in Rice False Smut Balls. Wang, Xiaohan,Fu, Xiaoxiang,Lin, Fengke,Sun, Weibo,Meng, Jiajia,Wang, Ali,Lai, Daowan,Zhou, Ligang,Liu, Yang. 2016

[3]New Ustilaginoidins from Rice False Smut Balls Caused by Villosiclava virens and Their Phytotoxic and Cytotoxic Activities. Sun, Weibo,Wang, Ali,Xu, Dan,Wang, Weixuan,Meng, Jiajia,Lai, Daowan,Zhou, Ligang,Dai, Jungui,Dai, Jungui,Liu, Yang.

[4]Ustiloxin G, a New Cyclopeptide Mycotoxin from Rice False Smut Balls. Wang, Xiaohan,Wang, Jian,Lai, Daowan,Wang, Weixuan,Zhou, Ligang,Dai, Jungui,Dai, Jungui,Liu, Yang. 2017

[5]Bioactive Bis-naphtho-gamma-pyrones from Rice False Smut Pathogen Ustilaginoidea virens. Lu, Shiqiong,Sun, Weibo,Meng, Jiajia,Wang, Ali,Wang, Xiaohan,Tian, Jin,Fu, Xiaoxiang,Lai, Daowan,Zhou, Ligang,Dai, Jungui,Dai, Jungui,Liu, Yang.

[6]Chemical Constituents of the Ethyl Acetate Extract from Diaphragma juglandis Fructus and Their Inhibitory Activity on Nitric Oxide Production In Vitro. Wang, Dan,Mu, Yan,Dong, Hongjing,Yan, Huijiao,Hao, Cui,Wang, Xiao,Wang, Dan,Zhang, Lisi. 2018

[7]Development of a Monoclonal Antibody-Based icELISA for the Detection of Ustiloxin B in Rice False Smut Balls and Rice Grains. Fu, Xiaoxiang,Wang, Ali,Wang, Xiaohan,Lin, Fengke,He, Lishan,Lai, Daowan,Zhou, Ligang,Wang, Baoming,Liu, Yang,Li, Qing X.. 2015

[8]A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples. Fu, Xiaoxiang,Wang, Xiaohan,Cui, Yongliang,Wang, Ali,Lai, Daowan,Wang, Baomin,Zhou, Ligang,Liu, Yang,Li, Qing X..

[9]Preparative isolation, quantification and antioxidant activity of dihydrochalcones from Sweet Tea (Lithocarpus polystachyus Rehd.). Sun, Yinshi,Liu, Zhengbo,Li, Wei.

[10]PREPARATIVE SEPARATION AND PURIFICATION OF SESQUITERPENOIDS FROM Tussilago farfara L. BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY. Wang, Daijie,Fang, Lei,Wang, Xiao,Qiu, Jiying,Huang, Luqi.

[11]Preparative isolation of cordycepin, N-6-(2-hydroxyethyl)-adenosine and adenosine from Cordyceps militaris by macroporous resin and purification by recycling high-speed counter-current chromatography. Zhang, Zhong,Tudi, Tuernisan,Liu, Yanfang,Zhou, Shuai,Feng, Na,Yang, Yan,Tang, Chuanhong,Tang, Qingjiu,Zhang, Jingsong,Tudi, Tuernisan,Tang, Qingjiu.

[12]Isolation and purification of flavonoid glucosides from Radix Astragali by high-speed counter-current chromatography. Xiao, Weihua,Han, Lujia,Shi, Bo.

[13]Application of high-speed counter-current chromatography and HPLC to separate and purify of three polyacetylenes from Platycodon grandiflorum. Chen, Bao,Liu, Zhengbo,Zhang, Yuwei,Wang, Yufang,Wang, Yanhua,Sun, Yinshi,Chen, Bao,Li, Wei,Wang, Yanhua,Sun, Yanjun. 2018

[14]Screening and separation of alpha-amylase inhibitors from Solanum nigrum with amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography. Cen, Yin,Chen, Xiaoqing,Xiao, Aiping,Liu, Liangliang. 2017

[15]Preparative Isolation of Two Prenylated Biflavonoids from the Roots and Rhizomes of Sinopodophyllum emodi by Sephadex LH-20 Column and High-Speed Counter-Current Chromatography. Sun, Yan-Jun,Pei, Li-Xin,Wang, Jun-Min,Zhang, Yan-Li,Gao, Mei-Ling,Ji, Bao-Yu,Sun, Yan-Jun,Pei, Li-Xin,Wang, Jun-Min,Zhang, Yan-Li,Gao, Mei-Ling,Ji, Bao-Yu,Wang, Kai-Bo,Wang, Kai-Bo,Sun, Yin-Shi.

[16]Isolation of bioactive components from Flaveria bidentis (L.) Kuntze using high-speed counter-current chromatography and time-controlled collection method. Wei, Yun,Zhang, Kai,Yin, Li,Du, Jilin,Zhang, Guoliang.

[17]Solvent gradient elution for comprehensive separation of constituents with wide range of polarity in Apocynum venetum leaves by high-speed counter-current chromatography. Zhang, Yuchi,Liu, Chunming,Qi, Yanjuan,Wu, Guimei,Li, Sainan,Zhang, Zhengkun.

[18]Frequency distribution of sensitivity of Ustilaginoidea virens to four EBI fungicides, prochloraz, difenoconazole, propiconazole and tebuconazole, and their efficacy in controlling rice false smut in Anhui Province of China. Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Yao, Jian,Li, Yun-Fei,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun. 2013

[19]Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice. Shan, Tijiang,Sun, Weibo,Liu, Hao,Gao, Shan,Lu, Shiqiong,Wang, Mingan,Sun, Wenxian,Zhou, Ligang,Chen, Zhiyi,Wang, Shu. 2012

[20]Characterization of mating-type loci in rice false smut fungus Villosiclava virens. Yu, Jun-jie,Yu, Mi-na,Yin, Xiao-le,Meng, Xiang-kun,Zhao, Jie,Huang, Lei,Liu, Yong-feng,Sun, Wen-xian,Huang, Li. 2015

作者其他论文 更多>>

微信小程序