Gene expression changes in chicken NLRC5 signal pathway associated with in vitro avian leukosis virus subgroup J infection

文献类型： 外文期刊

第一作者： Qiu, L. L.

作者： Qiu, L. L.;Xu, L.;Guo, X. M.;Li, Z. T.;Wan, F.;Chen, G. H.;Chang, G. B.;Liu, X. P.

作者机构：

关键词： Avian leukosis virus;Chicken embryo fibroblast;NLRC5;Signal pathway;Real-time PCR

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Nucleotide-binding oligomerization domain-like receptors (NLRs) play a key role in the innate immune response as pattern-recognition receptors. However, the role of NLRC5, which is a member of the NLR family, in NF-kappa B activation and MHC-I expression remains debatable. Infection with the J group avian leukosis virus (ALV-J) can result in immunosuppression and a subsequent increase in susceptibility to secondary infection. This results in huge economic losses to the poultry industry worldwide. Using quantitative real-time polymerase chain reaction (qRT-PCR), we investigated the mRNA expression levels of NLRC5 signal pathway-related genes in secondary chicken embryo fibroblasts 7 days after infection with ALV-J. The results indicated that, compared with the control groups, the expression levels of TLR7, MHC-I, and IL-18 increased significantly in the infected groups at 7 days post-infection (d.p.i.). The expression levels of NLRC5 and IL-6 were conspicuously downregulated at 7 d.p.i., but the expression levels of NF-kappa B, STAT1, and STAT3 were not significantly altered. These results suggest that NLRC5 and some genes involved in the NLRC5 pathway play a key role in antiviral immunity, typically the response to ALV-J infection. Moreover, MHC-I expression levels vary between different cell types.

分类号：

  • 相关文献

[1]A mutation in the NLRC5 promoter limits NF-kappa B signaling after Salmonella Enteritidis infection in the spleen of young chickens. Chang, Guobin,Ma, Teng,Xu, Lu,Wang, Hongzhi,Li, Zhiteng,Guo, Xiaomin,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[2]Expression patterns of NLRC5 and key genes in the STAT1 pathway following infection with Salmonella pullorum. Qiu, Lingling,Ma, Teng,Chang, Guobin,Guo, Xiaomin,Xu, Lu,Zhang, Yang,Zhao, Wenming,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[3]Toxicarioside A, isolated from tropical Antiaris toxicaria, blocks endoglin/TGF-beta signaling in a bone marrow stromal cell line. Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Guo, Jun-Li,Tan, Guang-Hong. 2012

[4]Functions and Application of the AP2/ERF Transcription Factor Family in Crop Improvement. Xu, Zhao-Shi,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi. 2011

[5]The antioxidant action and mechanism of selenizing Schisandra chinensis polysaccharide in chicken embryo hepatocyte. Yue, Chanjuan,Hou, Ranran,Tian, Weijun,Liu, Kuanhui,Wang, Deyun,Liu, Jiaguo,Wu, Yi,Hu, Yuanliang,Chen, Jin.

[6]Single nucleotide polymorphism variants within tva and tvb receptor genes in Chinese chickens. Liao, C. T.,Chen, S. Y.,Chen, W. G.,Liu, Y.,Sun, B. L.,Li, H. X.,Xie, Q. M.,Liao, C. T.,Xie, Q. M.,Zhang, H. M.,Qu, H.,Wang, J.,Shu, D. M.,Xie, Q. M..

[7]Avian leukosis virus subgroup A and B infection in wild birds of Northeast China. Li, Delong,Qin, Liting,Qi, Xiaole,Wang, Yongqiang,Wang, Xiaomei,Gao, Yulong,Li, Delong,Liu, Sidang,Gao, Honglei,Yang, Bo,Liu, Wansi,Zeng, Xiangwei.

[8]16S rRNA genes Illumina sequencing revealed differential cecal microbiome in specific pathogen free chickens infected with different subgroup of avian leukosis viruses. Ma, Xinxin,Xu, Chuantian,Cui, Ning,Ma, Xinxin,Wang, Qi,Li, Hongmei,Xu, Chuantian,Cui, Ning,Zhao, Xiaomin.

[9]Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. Yun, Bingling,Li, Delong,Zhu, Haibo,Liu, Wen,Qin, Liting,Liu, Zaisi,Wu, Guan,Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Wang, Xiaomei,Gao, Yulong. 2013

[10]A premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups B, D, and E. Chen, WeiGuo,Liu, Yang,Li, Xinjian,Li, Hongxing,Dai, Zhenkai,Yan, Yiming,Zhang, Xinheng,Lin, Wencheng,Ma, Jingyun,Xie, Qingmei,Chen, WeiGuo,Liu, Yang,Li, Xinjian,Li, Hongxing,Dai, Zhenkai,Yan, Yiming,Zhang, Xinheng,Lin, Wencheng,Ma, Jingyun,Xie, Qingmei,Li, Aijun,Shu, Dingming,Zhang, Huanmin,Chen, WeiGuo,Liu, Yang,Li, Xinjian,Li, Hongxing,Dai, Zhenkai,Yan, Yiming,Zhang, Xinheng,Lin, Wencheng,Ma, Jingyun,Xie, Qingmei,Chen, WeiGuo,Lin, Wencheng,Ma, Jingyun,Xie, Qingmei. 2017

[11]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[12]Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Lin, Zhibing,Zhang, Yanlei,Zhang, Houshuang,Zhou, Yongzhi,Cao, Jie,Zhou, Jinlin.

[13]Characterization of transgenic Poncirus trifoliata overexpressing the ferric chelate reductase gene CjFRO2 from Citrus junos. Peng, A. H.,Liu, X. F.,He, Y. R.,Xu, L. Z.,Lei, T. G.,Yao, L. X.,Cao, L.,Chen, S. C..

[14]Molecular Cloning and Expression Analysis of the Transcription Factor Gene JrCBF from Juglans regia L.. Xu, L.,Chen, X.,Zhang, L. S.,Liu, Q. Z..

[15]Identification and expression profile analysis of putative odorant-binding proteins in Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae). Gong, Zhong-Jun,Miao, Jin,Duan, Yun,Jiang, Yue-Li,Li, Tong,Wu, Yu-Qing.

[16]Histological and transcriptional characterization of a novel recessive genic male sterility mutant in sesame (Sesamum indicum L.). Wu, Kun,Liu, Hongyan,Zuo, Yang,Yang, Minmin,Zhao, Yingzhong.

[17]Real-time PCR quantification of latent infection of wheat powdery mildew in the field. Zhou, Yilin,Zeng, Xiaowei,Duan, Xiayu,Cao, Xueren,Luo, Yong,Song, Yuli,Wang, Baotong.

[18]Comparison of Five Endogenous Reference Genes for Specific PCR Detection and Quantification of Brassica napus. Wu, Gang,Zhang, Li,Wu, Yuhua,Cao, Yinglong,Lu, Changming.

[19]Quantitative determination of soybean meal content in compound feeds: comparison of near-infrared spectroscopy and real-time PCR. Li, Hui,Lv, Xiaowen,Wang, Jing,Li, Junguo,Yang, Haifeng,Qin, Yuchang.

[20]Development of an Event-Specific Detection Method for Genetically Modified Maize IE034 by Quantitative Real-Time PCR. Li, Feiwu,Zhang, Shihong,Li, Feiwu,Long, Likun,Yan, Wei,Li, Congcong,Xu, Junfeng,Liu, Yunjun.

作者其他论文 更多>>

微信小程序