Development of an in vitro bulbil-induction system for Lilium lancifolium and the impact of exogenous auxin on bulbil formation

文献类型： 外文期刊

第一作者： Tang, Yuchao

作者： Tang, Yuchao;Luo, Kang;Bai, Jingyi;Zhang, Wenliang;Ming, Jun;Tang, Yuchao;Bai, Jingyi

作者机构：

关键词： Lilium lancifolium; Bulbil-induction system; Auxin; Auxin inhibitors; In vitro

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN： 0304-4238

年卷期： 2025 年 340 卷

页码：

收录情况： SCI

摘要： Lilium lancifolium is one of the most widely cultivated lily species in China, valued for its ornamental, edible, and medicinal properties. Bulbils, which are specialized vegetative organs formed in the leaf axils of L. lancifolium, play a crucial role in its reproduction. However, the mechanisms underlying bulbil formation in L. lancifolium have not been elucidated. In this study, we established an efficient and stable in vitro induction system for bulbil formation using the bulbs of L. lancifolium sprouted at 4 degrees C as materials. Additionally, we systematically investigated the effects of exogenous auxins and auxin inhibitors on bulbil development through a combination of morphological and histological observations, as well as analyses of hormone levels and gene expression. The results indicated that low concentrations of exogenous IAA not only increased the number of bulbils formed per leaf axil but also reduced the time required for bulbil initiation. Conversely, auxin inhibitors suppressed this process, with 2,3,5-triiodobenzoic acid (TIBA) exhibiting the most pronounced inhibitory effect. Phytohormone analysis reviewed that auxin (IAA), cytokinin (ZRs) and gibberellin (GA3) may be involved in bulbil formation. Early auxin signaling is required for the initiation of axillary meristem formation during bulbil development, while the formation of bulbil primordia necessitates lower concentrations of auxin accumulation. Cytokinin appears to promote bulbil formation, and a lower IAA/ZR ratio may be advantageous for axillary meristem (AM) development. Gene expression data suggest that auxin- and cytokinin-related genes are implicated in bulbil initiation. The present study provided a viable system for investigating the mechanisms underlying bulbil formation in lilies. And the results laid solid foundation of the regulation of bulbil formation in plants.

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