Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis

文献类型： 外文期刊

第一作者： Lang, Kun-Ling

作者： Lang, Kun-Ling;Shen, Chang-Peng;Wan, Fang-Hao;Chu, Dong;Fu, Hai-Bin;Wan, Fang-Hao

作者机构：

关键词： expressed sequence tags;microsatellite marker;normalized cDNA library;Phenacoccus solenopsis

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2015 年 22 卷 6 期

页码：

收录情况： SCI

摘要： The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.

分类号：

  • 相关文献

[1]Construction of a Full-Length cDNA Library of Gossypium hirsutum L. and Identification of Two MADS-Box Genes. Wang Li-na,Wu Dong,Yu Shu-xun,Fan Shu-li,Song Mei-zhen,Pang Chao-you,Liu Jun-jie. 2011

[2]Large-scale sequencing of normalized full-length cDNA library of soybean seed at different developmental stages and analysis of the gene expression profiles based on ESTs. Sha, Ai-Hua,Li, Chen,Yan, Xiao-Hong,Shan, Zhi-Hui,Zhou, Xin-An,Jiang, Mu-Lan,Mao, Han,Chen, Bo,Wan, Xia,Wei, Wen-Hui.

[3]Chlorophyll Content and Chlorophyll Fluorescence in Tomato Leaves Infested With an Invasive Mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae). Huang, Jun,Zhang, Juan,Li, Ming-Jiang,Zhang, Peng-Jun,Lu, Yao-Bin,Huang, Fang. 2013

[4]Effects of temperature and host stage on the parasitization rate and offspring sex ratio of Aenasius bambawalei Hayat in Phenacoccus solenopsis Tinsley. Zhang, Juan,Huang, Jun,Xia, Tianfeng,Huang, Jun,Lu, Yaobin. 2016

[5]Temperature-and Relative Humidity-Dependent Life History Traits of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) on Hibiscus rosa-sinensis (Malvales: Malvaceae). Yang, L.,Huang, L. F.,Wang, W. L.,Hu, Y.,Jiang, J. J.,Zhou, Z. S.,Hu, Y..

[6]EPG waveform characteristics of solenopsis mealybug stylet penetration on cotton. Huang, Fang,Zhang, Pengjun,Zhang, Jinming,Lu, Yaobin,Tjallingii, W. Freddy,Tjallingii, W. Freddy,Lin, Jintian. 2012

[7]Effects of a Native Ant, Pristomyrmex pungens (Hymenoptera: Formicidae) on the Population Dynamics and Spatial Distribution of the Invasive Mealybug Phenacoccus solenopsis (Hemiptera: Pseudococcidae). Huang, Jun,Wei, Hong-xu,Li, Ming-jiang,Zhang, Juan.

[8]Changes in the photosynthetic characteristics of cotton leaves infested by invasive mealybugs tended by native ant species. Huang, Jun,Zhang, Juan.

[9]Biological Characteristics and Chemical Control of the Invasive Mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae) on Tomato in the Laboratory. Huang, Jun,Yu, Yong-Ming,Zhang, Juan,Luan, Jun-Bo,Lu, Yao-Bin.

[10]Do organic silicon and imidacloprid synergistically induce toxicity to the new invasive mealybug Phenacoccus solenopsis Tinsley on Portulaca grandiflora plants?. Huang, Jun,Zhang, Juan,Li, Mingjiang,Wang, Daoze,Zhang, Lili,Xu, Yijuan.

[11]Synergistic Effect of Organic Silicon Surfactant in Chemical Control of the New Invasive Mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) in China. Zhang, Juan,Huang, Jun.

[12]Host Plant Probing Analysis Reveals Quick Settlement of the Solenopsis Mealybug During Host Shift. Huang, Fang,Zhang, Zhijun,Li, Weidi,Lin, Wencai,Zhang, Pengjun,Zhang, Jinming,Bei, Yawei,Lu, Yaobin,He, Yueping.

[13]Identification of Expressed Resistance Gene Analogs from Peanut (Arachis hypogaea L.) Expressed Sequence Tags. Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Chen, Xiaoping,Culbreath, Albert K.,Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Varshney, Rajeev K.,Chen, Xiaoping,Guo, Baozhu. 2013

[14]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li#2;,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[15]Immune gene discovery by expressed sequence tag (EST) analysis of hemocytes in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei. 2013

[16]DEVELOPMENT AND CHARACTERIZATION OF 41 NOVEL EST-SSR MARKERS FOR PISUM SATIVUM (LEGUMINOSAE). Xu, Sheng-Chun,Gong, Ya-Ming,Hu, Qi-Zan,Zhang, Gu-Wen,Mao, Wei-Hua,Fu, Wan,Xian, Qiang-Qiang,Fu, Wan,Xian, Qiang-Qiang. 2012

[17]Identification of differentially expressed genes in Mongolian sheep ovaries by suppression subtractive hybridization. He, Xiaolong,Wang, Feng,Tian, Chunying,Rong, Weiheng,Liu, Yongbin,He, Xiaolong,Li, Bei. 2012

[18]Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils. Jiang, YQ,Ma, RC. 2003

[19]SSR data mined from expressed sequence tags of phytoparasitic nematodes. Huang, Q. X.,Cheng, X. Y.,Xu, R. M.,Xie, B. Y.. 2010

[20]Erwinia carotovora ssp. Carotovora infection induced "defense lignin" accumulation and lignin biosynthetic gene expression in chinese cabbage (Brassica rapa L. ssp pekinensis). Zhang, Song-He,Yang, Qing,Ma, Rong-Cai. 2007

作者其他论文 更多>>

微信小程序