Generation of Internal-Image Functional Aptamers of Okadaic Acid via Magnetic-Bead SELEX

文献类型： 外文期刊

第一作者： Lin, Chao

作者： Lin, Chao;Liu, Zeng-Shan;Li, Lin;Hu, Pan;Gong, Sheng;Li, Yan-Song;Cui, Cheng;Wu, Zong-Cheng;Gao, Yang;Zhou, Yu;Ren, Hong-Lin;Lu, Shi-Ying;Wang, Dong-Xu;Lin, Chao

作者机构：

关键词： okadaic acid;SELEX;internal-image function

期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）

ISSN： 1660-3397

年卷期： 2015 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Okadaic acid ( OA) is produced by Dinophysis and Prorocentrum dinoflagellates and primarily accumulates in bivalves, and this toxin has harmful effects on consumers and operators. In this work, we first report the use of aptamers as novel non- toxic probes capable of binding to a monoclonal antibody against OA ( OA- mAb). Aptamers that mimic the OA toxin with high affinity and selectivity were generated by the magnetic bead- assisted systematic evolution of ligands by exponential enrichment ( SELEX) strategy. After 12 selection rounds, cloning, sequencing and enzyme- linked immunosorbent assay ( ELISA) analysis, four candidate aptamers ( O24, O31, O39, O40) were selected that showed high affinity and specificity for OA- mAb. The affinity constants of O24, O31, O39 and O40 were 8.3 X 108 M - 1, 1.47 X 109 M - 1, 1.23 X 109 M - 1 and 1.05 X 109 M - 1, respectively. Indirect competitive ELISA was employed to determine the internal- image function of the aptamers. The results reveal that O31 has a similar competitive function as free OA toxin, whereas the other three aptamers did not bear the necessary internal- image function. Based on the derivation of the curvilinear equation for OA/ O31, the equation that defined the relationship between the OA toxin content and O31 was Y = 2.185X - 1.78. The IC50 of O31 was 3.39 ng center dot mL - 1, which was close to the value predicted by the OA ELISA ( IC50 = 4.4 ng - mL - 1); the IC10 was 0.33 ng center dot mL - 1. The above data provides strong evidence that internal- image functional aptamers could be applicable as novel probes in a non- toxic assay.

分类号：

  • 相关文献

[1]Isolation and Identification of the DNA Aptamer Target to Acetamiprid. He, Jiang,Liu, Yuan,Liu, Xianjin,He, Jiang,Fan, Mingtao.

[2]Occurrence of marine algal toxins in oyster and phytoplankton samples in Daya Bay, South China Sea. Jiang, Tao,Tan, Zhijun,Wu, Haiyan,Jiang, Tao,Liu, Lei,Zhang, Jing,Jiang, Tianjiu,Lu, Songhui,Liu, Lei,Li, Yang.

作者其他论文 更多>>

微信小程序