Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties

文献类型： 外文期刊

第一作者： Jin, Jing

作者： Jin, Jing;Zhao, Lan;Gao, Zhongshan;Jin, Jing;Gan, Kexin;Zhao, Lan;Jia, Huijuan;Zhu, Yifan;Gao, Zhongshan;Li, Xiongwei;Ye, Zhengwen;Yang, Zhaowei;Cao, Ke;Wang, Zhiqiang;Yu, Mingliang;Zhang, Yuyan;Ma, Zhisheng;Liu, Hangkong;Arus, Pere;Akkerdaas, Jaap H.;Gao, Zhongshan;van Ree, Ronald;Akkerdaas, Jaap H.;Gao, Zhongshan;van Ree, Ronald

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关键词： ELISA; hypoallergenic varieties; peach allergy; protein quantification; Pru p 1; 酶 联 免 疫 吸 附 测 试 低 致 敏 品 种 桃 过 敏 蛋 白 定 量 Pru p 1

期刊名称：CLINICAL AND TRANSLATIONAL ALLERGY （ 影响因子：5.871； 五年影响因子：4.891 ）

ISSN：

年卷期： 2021 年 11 卷 3 期

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收录情况： SCI

摘要： Background Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme-linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction. Results A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20-73.44 mu g/g, fresh weight), being very low in the pulp (0.05-9.62 mu g/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 mu g/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late-ripening yellow flesh varieties were usually highly allergenic. Conclusion Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, "Zi Xue Tao," "Wu Yue Xian," and "May Fire," were identified, which could be useful in trials for peach allergy patients.

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