A novel bifunctional GH51 exo-alpha-L-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp A4 with significant biomass-degrading capacity

文献类型： 外文期刊

第一作者： Yang, Wenxia

作者： Yang, Wenxia;Bai, Yingguo;Yang, Peilong;Luo, Huiying;Huang, Huoqing;Meng, Kun;Shi, Pengjun;Wang, Yaru;Yao, Bin

作者机构：

关键词： Alicyclobacillus sp A4;alpha-l-Arabinofuranosidase (Abf);Xylanase;Glycosyl hydrolase (GH);Synergism

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN： 1754-6834

年卷期： 2015 年 8 卷

页码：

收录情况： SCI

摘要： Background: Improving the hydrolytic performance of xylanolytic enzymes on arabinoxylan is of importance in the ethanol fermentation industry. Supplementation of debranching (arabinofuranosidase) and depolymerizing (xylanase) enzymes is a way to address the problem. In the present study, we identified a bifunctional alpha-L-arabinofuranosidase/endo-xylanase (Ac-Abf51A) of glycoside hydrolase family 51 in Alicyclobacillus sp. strain A4. Its biochemical stability and great hydrolysis efficiency against complex biomass make it a potential candidate for the production of biofuels. Results: The gene encoding Ac-Abf51A was cloned. The comparison of its sequence with reference proteins having resolved 3D-structures revealed nine key residues involved in catalysis and substrate-binding interaction. Recombinant Ac-Abf51A produced in Escherichia coli showed optimal activity at pH 6.0 and 60 degrees C with 4-nitrophenyl-alpha-L- arabinofuranoside as the substrate. The enzyme exhibited an exo-type mode of action on polyarabinosides by catalyzing the cleavage of alpha-1,2-and alpha-1,3-linked arabinofuranose side chains in sugar beet arabinan and water-soluble wheat arabinoxylan and alpha-1,5-linked arabinofuranosidic bonds in debranched sugar beet arabinan. Surprisingly, it had capacity to release xylobiose and xylotriose from wheat arabinoxylan and was active on xylooligosaccharides (xylohexaose 1.2/mM/min, xylopentaose 6.9/mM/min, and xylotetraose 19.7/mM/min), however a lower level of activity. Moreover, Ac-Abf51A showed greater synergistic effect in combination with xylanase (2.92-fold) on wheat arabinoxylan degradation than other reported enzymes, for the amounts of arabinose, xylose, and xylobiose were all increased in comparison to that by the enzymes acting individually. Conclusions: This study for the first time reports a GH51 enzyme with both exo-alpha-L-arabinofuranosidase and endoxylanase activities. It was stable over a broad pH range and at high temperature, and showed greater synergistic effect with xylanase on the degradation of wheat arabinoxylan than other counterparts. The distinguished synergy might be ascribed to its bifunctional alpha-L-arabinofuranosidase/xylanase activity, which may represent a possible way to degrade biomass at lower enzyme loadings.

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