Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides

文献类型： 外文期刊

第一作者： Bai, Wenhui

作者： Bai, Wenhui;Zhu, Chao;Liu, Jinchuan;Yan, Mengmeng;Yang, Shuming;Chen, Ailiang;Bai, Wenhui;Zhu, Chao;Liu, Jinchuan;Yan, Mengmeng;Yang, Shuming;Chen, Ailiang

作者机构：

关键词： Aptamer;Gold nanoparticle;Colorimetric assay;Organophosphorous pesticide;Rapid detection

期刊名称：ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY （ 影响因子：3.742； 五年影响因子：3.866 ）

ISSN： 0730-7268

年卷期： 2015 年 34 卷 10 期

页码：

收录情况： SCI

摘要： Fast immunoassay-based screening methods are unavailable for most small-molecule pesticides because of a lack of immunogenicity and the difficulty in obtaining antibodies by animal immunization. Aptamers are single-stranded DNA molecules selected through an in vitro process, which can bind to any target including nonimmunogenic small molecules with high affinity and specificity. Although various aptamer-based sensing methods have been developed for antibiotics, microorganisms, heavy metal ions, and biotoxins, there are few reports on aptamer-based methods for quick detection of organophosphorous pesticides. The gold (Au) nanoparticle (AuNP) colorimetric assay is a widely utilized rapid detection method because of properties such as easy operation and visualized results. In the present study, organophosphorous pesticide aptamers were adsorbed on the surface of AuNPs to stabilize the AuNP solution against high concentrations of salt to prevent AuNP aggregation. After the addition of targets, the aptamers binding to the targets are detached from the AuNPs, resulting in aggregation of AuNPs and a color change from red to purple-blue. The proposed method can detect 6 organophosphorous pesticides with good recoveries from 72% to 135% in environmental river water samples. The present study provides a new way for simple, rapid, and multiplex detection of organophosphorous pesticides. Environ Toxicol Chem 2015;34:2244-2249. (c) 2015 SETAC

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