Assessing Susceptibility of Grapevine Cultivars to Latent Plasmopara viticola Infections Using the Molecular Disease Index

文献类型： 外文期刊

第一作者： Yang, Lujia

作者： Yang, Lujia;Deng, Jie;Sun, Qiuyu;Lv, Xuan;Ma, Zhanhong;Yang, Lujia;Chu, Bingyao;Shen, Zhaomeng;Zhan, Jiasui

作者机构：

关键词： molecular disease index; multiple real-time PCR; Plasmopara viticola; susceptibility; Vitis vinifera

期刊名称：PHYTOPATHOLOGY （ 影响因子：3.1； 五年影响因子：3.5 ）

ISSN： 0031-949X

年卷期： 2025 年 115 卷 4 期

页码：

收录情况： SCI

摘要： Traditional assessments of grapevine susceptibility to grapevine downy mildew (GDM) caused by Plasmopara viticola rely on the visual evaluation of leaf symptoms. In this study, we used a well-established quantitative real-time PCR TaqMan assay (real-time PCR) to quantify the number of P. viticola infecting 12 grapevine cultivars under controlled conditions. The molecular disease index (MDI), derived from molecular detection methods, reflects the relative abundance of pathogens in plant tissues during the latent infection phase. Our application of the MDI revealed a progressive increase in latent P. viticola levels over time, indicating dynamic levels of latent P. viticola infection across the inoculation processes. We calculated the area under the disease progress curve in terms of the MDI (AUDPCMDI) to evaluate the susceptibility of each cultivar to GDM. Cultivars with lower AUDPCMDI values consistently exhibited reduced pathogen establishment, suggesting higher levels of innate resistance. Correlation analysis revealed a significant correlation between the visual disease index and the AUDPCMDI values (r = 0.790, P = 0.002), indicating that higher levels of latent P. viticola infections were associated with higher disease severity. Grapevine cultivars were clustered into distinct groupings, indicating variability in their susceptibility to the pathogen. Cultivars with similar levels of susceptibility were grouped, highlighting that the real-time PCR assay used in this study represents a robust, rapid, and standardized method for quantifying pathogens, which significantly improves the efficiency of evaluating the susceptibility of grapevine cultivars to GDM. This quantitative protocol provides practical guidelines for selecting resistant cultivars and implementing effective disease management strategies.

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