Genome-wide analysis of WRKY gene family in high-CBD hemp (Cannabis sativa L.) and identification of the WRKY genes involved in abiotic stress responses and regulation cannabinoid accumulation

文献类型： 外文期刊

第一作者： Yu, Jiangshan

作者： Yu, Jiangshan;Cao, Xue;Mi, Yaolei;Sun, Wei;Meng, Xiangxiao;Chen, Weiqiang;Xie, Xuehua;Wang, Sifan;Li, Jun;Yang, Wei;Chen, Sha;Chen, Shilin;Wan, Huihua;Chen, Shilin;Wan, Huihua;Mi, Yaolei;Wan, Huihua;Wan, Huihua

作者机构：

关键词： WRKY; Hemp; Abiotic stress; Cannabinoids

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.9； 五年影响因子：6.0 ）

ISSN： 0926-6690

年卷期： 2024 年 210 卷

页码：

收录情况： SCI

摘要： Cannabinoids are vital metabolites produced by Cannabis sativa. The growing market demand for cannabinoids has made C. sativa a prominent research focus. The biosynthetic pathway of cannabinoids has been extensively investigated, but the information on the transcription factors (TFs) involved in cannabinoids biosynthesis remains limited. In this study, a total of 48 CsWRKYS were identified in high-cannabidiol (CBD) hemp using a combination of two-way blast, plantTFDB prediction, and CDD batching methods. These CsWRKYs exhibited uneven distribution across ten chromosomes, and encoded 162-728 amino acids. Phylogenetic analysis revealed that CsWRKY genes were categorized into three groups, with group II further divided into five subgroups. Members within each subfamily exhibited similar conserved motifs and exon-intron structures. Furthermore, gene expression analysis in various tissues, accompanied by cis-regulatory elements analysis, and alternative splicing analysis, provided insight into the potential roles of CsWRKYs in hemp growth and development. Notably, the expression of group III CsWRKY genes (CsWRKY1/2/20/26/28) was induced by salt, drought, and cold stress treatments. Metabolic analysis revealed an increase in the contents of cannabinoids under these treatments. Furthermore, CsWRKY20 and CsWRKY28 were proved to be positive regulatory factors of cannabinoids biosynthesis pathway. Altogether, the results of this study significantly contributes to understand the function of WRKY genes in response to abiotic stress, and their involvement in the transcriptional regulation of cannabinoid biosynthesis in high-CBD hemp.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Impact of residual antibiotics on microbial decomposition of livestock manures in Eutric Regosol: Implications for sustainable nutrient recycling and soil carbon sequestration

    作者：Fang, Linfa;Lakshmanan, Prakash;Su, Xiaoxuan;Shi, Yujia;Chen, Zheng;Zhang, Yu;Xiao, Ran;Chen, Xinping;Lakshmanan, Prakash;Wu, Junxi;Lakshmanan, Prakash;Lakshmanan, Prakash;Sun, Wei

    关键词：Residual antibiotics; Livestock manure decomposition; Microbial community; Co -occurrence network; Enzyme activities

  • Plant-derived strategies to fight against severe acute respiratory syndrome coronavirus 2

    作者：Li, Wenkang;Ding, Tianze;Chang, Huimin;Peng, Yuanchang;Li, Jun;Liang, Xin;Ma, Huixin;Li, Fuguang;Ren, Maozhi;Wang, Wenjing;Li, Wenkang;Ding, Tianze;Chang, Huimin;Peng, Yuanchang;Li, Jun;Liang, Xin;Ma, Huixin;Li, Fuguang;Ren, Maozhi;Wang, Wenjing;Liang, Xin;Li, Fuguang;Wang, Wenjing;Ren, Maozhi;Wang, Wenjing

    关键词：SARS-CoV-2; Plant natural products; Antiviral drugs; Molecular farming; Structural biology; Synthetic biology

  • Genome-Wide Analysis of the TCP Transcription Factor Gene Family in Pepper (Capsicum annuum L.)

    作者：Dong, Zeyu;Hao, Yupeng;Zhao, Yongyan;Tang, Wenchen;Wang, Xueqiang;Li, Jun;Wang, Luyao;Hu, Yan;Guan, Xueying;Zhang, Zhiyuan;Gu, Fenglin;Gu, Fenglin;Liu, Ziji;Liu, Ziji

    关键词：Capsicum annuum L.; TCP transcription factors; shoot branching; hormone response; abiotic stress

  • Extreme drought impacts have been underestimated in grasslands and shrublands globally

    作者：Smith, Melinda D.;Knapp, Alan K.;Ohlert, Timothy;Smith, Melinda D.;Knapp, Alan K.;Wilkins, Kate D.;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Wilfahrt, Peter;Anderson, Maggie;Borer, Elizabeth T.;Isbell, Forest;Seabloom, Eric W.;Collins, Scott L.;Sala, Osvaldo E.;Jordan, Samuel;Dukes, Jeffrey S.;Phillips, Richard P.;Beier, Claus;Yahdjian, Laura;Biancari, Lucio;Bondaruk, Viviana Florencia;Onatibia, Gaston R.;Pineiro-Guerra, Juan Manuel;Gherardi, Laureano A.;Larsen, Klaus Steenberg;Fraser, Lauchlan H.;Jentsch, Anke;Niu, Yujie;Loik, Michael E.;Maestre, Fernando T.;Valdecantos, Alejandro;Maestre, Fernando T.;Gozalo, Beatriz;Maturano-Ruiz, Adrian;Valdecantos, Alejandro;Power, Sally A.;Chieppa, Jeff;Nielsen, Uffe N.;Yu, Qiang;Felton, Andrew J.;Munson, Seth M.;Luo, Yiqi;Bauerle, Taryn;Abdoli, Hamed;Abedi, Mehdi;Bahalkeh, Khadijeh;Alados, Concepcion L.;Castillo-Garcia, Miguel;Pueyo, Yolanda;Alberti, Juan;Bruschetti, Carlos Martin;Cardoni, Augusto;Daleo, Pedro;Iribarne, Oscar;Alon, Moshe;Cohen, Ofer;Sternberg, Marcelo;An, Hui;Anacker, Brian;Auge, Harald;Auge, Harald;Eisenhauer, Nico;Hoss, Daniela;Roscher, Christiane;Sunnemann, Marie;Bachle, Seton;Bachle, Seton;Bahn, Michael;Ingrisch, Johannes;Batbaatar, Amgaa;Cahill, James F., Jr.;Batbaatar, Amgaa;Bork, Edward W.;Carlyle, Cameron N.;Behn, Kai;Beil, Ilka;Kreyling, Juergen;Malyshev, Andrey V.;Blindow, Irmgard;Dahlke, Sven;Byrne, Kerry M.;Calvo, Dianela A.;Leder, Cintia V.;Peter, Guadalupe;Carbognani, Michele;Forte, T'ai G. W.;Petraglia, Alessandro;Chang, Scott X.;Cianciaruso, Marcus V.;Guidoni-Martins, Karlo G.;Nobre, Paola;Cordeiro, Amanda L.;Cusack, Daniela F.;Dietterich, Lee H.;D'Antonio, Carla M.;Dietterich, Lee H.;Doherty, Tim S.;Greenville, Aaron C.;Wardle, Glenda M.;Dubbert, Maren;Ebeling, Anne;Eisenhauer, Nico;Fischer, Felicia M.;Hoss, Daniela;Sunnemann, Marie;Hoss, Daniela;Pillar, Valerio D.;Fischer, Felicia M.;Gebauer, Tobias;Anacker, Brian;Haugum, Siri Vatso;Vandvik, Vigdis;Hautier, Yann;Hefting, Mariet;Henry, Hugh A. L.;Johnson, Yari;Kelly, Eugene F.;Kimmel, Kaitlin;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kubert, Angelika;Werner, Christiane;Lamb, Eric G.;Larson, Julie;O'Connor, Rory C.;Lawson, Jason;Plowes, Robert M.;Linstadter, Anja;Orban, Ildiko;Liu, Jielin;Liu, Shirong;Longo, Grisel;Loydi, Alejandro;Rodriguez, Dana Aylen;Scarfo, Maria Cecilia;Luan, Junwei;Wang, Yi;Luan, Junwei;Wang, Yi;Lubbe, Frederick Curtis;Wiehl, Georg;Mackie-Haas, Kathleen;Stampfi, Andreas;Merchant, Thomas;Metcalfe, Daniel B.;Mori, Akira S.;Mori, Akira S.;Mudongo, Edwin;Newman, Gregory S.;Souza, Lara;Nimmo, Dale;Ogaya, Roma;Penuelas, Josep;Osborne, Brooke;Otfinowski, Rafael;Partel, Meelis;Pineiro-Guerra, Juan Manuel;Peri, Pablo L.;Picon-Cochard, Catherine;Pineiro-Guerra, Juan Manuel;Ploughe, Laura W.;Portales-Reyes, Cristy;Reed, Sasha C.;Ritchie, Euan G.;Roscher, Christiane;Sanchez, Ana M.;Santo, Braulio A.;Shi, Baoku;Sun, Wei;Souza, Lara;Standish, Rachel J.;Zeiter, Michaela;Stampfi, Andreas;Zeiter, Michaela;Standish, Rachel J.;Tedder, Michelle;Thorvaldsen, Pal;Tian, Dashuan;Zhu, Juntao;Zong, Ning;Tielborger, Katja;van den Brink, Liesbeth;Vankoughnett, Mathew R.;Velle, Liv Guri;Wang, Changhui;Wei, Cunzheng;Williams, Jennifer L.;Wolf, Amelia A.;Zhang, Fawei;Zuo, Xiaoan;Zuo, Xiaoan;Gebauer, Tobias;Ploughe, Laura W.

    关键词：climate extreme; Drought-Net; International Drought Experiment; productivity

  • Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells

    作者：Nan, Fulong;Wang, Hui;Jiang, Shasha;Zhang, Shuyun;Zhang, Xianjuan;Liu, Fengjun;Li, Jun;Zhou, Xiaoqiong;Niu, Delei;Wang, Bin;Nan, Wenlong;Yan, Xin;Li, Yiquan;Wang, Wei;Shi, Ning;Jin, Ningyi;Xie, Changzhan;Zhang, He;Lu, Huijun;Yu, Zhongjie;Cui, Xiaoni

    关键词：Newcastle disease virus; Dendritic cells; Interleukin-12 (IL-12); T cells; Immunosuppression

  • Ultrasonography screening of hepatic cystic echinococcosis in sheep flocks used for evaluating control progress in a remote mountain area of Hejing County, Xinjiang

    作者：Qi, Xinwei;Song, Tao;Li, Zhao;Dong, Jian;Dawuti, Wubulitalifu;Dou, Jingrui;Li, Jun;Wen, Hao;Zhang, Wenbao;Zhang, Zhuangzhi;Jiang, Tao;Wu, Chuanchuan;Zhang, Wenbao;Guo, Gang

    关键词：Ultrasonography; Cystic echinococcosis; Echinococcus granulosus; Sheep flocks; Active cysts

  • miR-329b-5p Affects Sheep Intestinal Epithelial Cells against Escherichia coli F17 Infection

    作者：Xu, Yeling;Chen, Weihao;Sun, Wei;Yang, Huiguo;Song, Zhenghai;Wang, Yeqing;Su, Rui;Mwacharo, Joram M.;Lv, Xiaoyang;Sun, Wei;Lv, Xiaoyang;Sun, Wei

    关键词：Escherichia coli F17; micro RNA; sheep diarrhea; intestinal epithelial cells

微信小程序