A member of the HSP90 family from ovine Babesia in China: molecular characterization, phylogenetic analysis and antigenicity

文献类型： 外文期刊

第一作者： Guan, Guiquan

作者： Guan, Guiquan;Liu, Junlong;Liu, Aihong;Li, Youquan;Niu, Qingli;Gao, Jinliang;Luo, Jianxun;Yin, Hong;Guan, Guiquan;Chauvin, Alain;Moreau, Emmanuelle;Guan, Guiquan;Chauvin, Alain;Moreau, Emmanuelle;Yin, Hong

作者机构：

关键词： Babesia motasi;Babesia sp BQ1 (Lintan);HSP90;phylogenetic analysis;antigenicity

期刊名称：PARASITOLOGY （ 影响因子：3.234； 五年影响因子：3.118 ）

ISSN： 0031-1820

年卷期： 2015 年 142 卷 11 期

页码：

收录情况： SCI

摘要： Heat shock protein 90 (HSP90) is a key component of the molecular chaperone complex essential for activating many signalling proteins involved in the development and progression of pathogenic cellular transformation. A Hsp90 gene (BQHsp90) was cloned and characterized from Babesia sp. BQ1 (Lintan), an ovine Babesia isolate belonging to Babesia motasi-like group, by screening a cDNA expression library and performing rapid amplification of cDNA ends. The full-length cDNA of BQHsp90 is 2399 bp with an open reading frame of 2154 bp encoding a predicted 83 kDa polypeptide with 717 amino acid residues. It shows significant homology and similar structural characteristics to Hsp90 of other apicomplex organisms. Phylogenetic analysis, based on the HSP90 amino acid sequences, showed that the Babesia genus is clearly separated from other apicomplexa genera. Five Chinese ovine Babesia isolates were divided into 2 phylogenetic clusters, namely Babesia sp. Xinjiang (previously designated a new species) cluster and B. motasi-like cluster which could be further divided into 2 subclusters (Babesia sp. BQ1 (Lintan)/Babesia sp. Tianzhu and Babesia sp. BQ1 (Ningxian)/Babesia sp. Hebei). Finally, the antigenicity of rBQHSP90 protein from prokaryotic expression was also evaluated using western blot and enzyme-linked immunosorbent assay (ELISA).

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