Protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 and rhomboid 5 proteins against acute and chronic toxoplasmosis in mice

文献类型： 外文期刊

第一作者： Zhang, Nian-Zhang

作者： Zhang, Nian-Zhang;Xu, Ying;Wang, Meng;Chen, Jia;Huang, Si-Yang;Zhu, Xing-Quan;Xu, Ying;Petersen, Eskild;Huang, Si-Yang;Zhu, Xing-Quan

作者机构：

关键词： DNA vaccine;immune response;protective immunity;ROM4;ROM5;Toxoplasma gondii

期刊名称：EXPERT REVIEW OF VACCINES （ 影响因子：5.217； 五年影响因子：5.106 ）

ISSN： 1476-0584

年卷期： 2015 年 14 卷 9 期

页码：

收录情况： SCI

摘要： Objectives: To evaluate the protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 (ROM4) and rhomboid 5 (ROM5) proteins against acute and chronic toxoplasmosis. Methods: DNA vaccines (pVAX-TgROM5 and pVAX-TgROM4) were constructed and their immunogenicity evaluated in Kunming mice. Results: Mice vaccinated with pVAX-TgROM5 or pVAX-TgROM4 elicited strong Th1-type humoral and cellular responses, with higher level of IgG antibody titers (the predominance of IgG2a production), and increased levels of CD4(+) and CD8(+) T cells and cytokines IFN-gamma, IL-2, IL-12 (p70) and IL-23. Mice vaccinated with pVAX-TgROM5 (11 days) showed a significantly longer survival time compared with controls (8 days) (p < 0.05) after lethal challenge. Brain cyst numbers of mice vaccinated with pVAX-TgROM5 and pVAX-TgROM4 reduced significantly (p < 0.05) (72.04 and 44.08%, respectively) compared with control groups after chronic challenge. Conclusion: The pVAX-TgROM5 showed a better protective efficacy against acute and chronic toxoplasmosis compared to pVAX-TgROM4.

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