Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels

文献类型： 外文期刊

第一作者： Lian, Tong

作者： Lian, Tong;Chen, Maoran;Liang, Qiuju;Liu, Fang;Meng, Hongyan;Xu, Bosi;Chen, Jinfeng;Zhang, Chunyi;Jiang, Ling;Guo, Wenzhu;Li, Jinglai;Liang, Qiuju;Zhang, Chunyi;Jiang, Ling;Chen, Jinfeng

作者机构：

关键词： Maize;Folate metabolism;C1 metabolism;Expression pattern;Folate profiling

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2015 年 15 卷

页码：

收录情况： SCI

摘要： Background: Maize is a major staple food crop globally and contains various concentrations of vitamins. Folates are essential water-soluble B-vitamins that play an important role as one-carbon (C1) donors and acceptors in organisms. To gain an understanding of folate metabolism in maize, we performed an intensive in silico analysis to screen for genes involved in folate metabolism using publicly available databases, followed by examination of the transcript expression patterns and profiling of the folate derivatives in the kernels of two maize inbred lines. Results: A total of 36 candidate genes corresponding to 16 folate metabolism-related enzymes were identified. The maize genome contains all the enzymes required for folate and C1 metabolism, characterized by highly conserved functional domains across all the other species investigated. Phylogenetic analysis revealed that these enzymes in maize are conserved throughout evolution and have a high level of similarity with those in sorghum and millet. The LC-MS analyses of two maize inbred lines demonstrated that 5-methyltetrahydrofolate was the major form of folate derivative in young seeds, while 5-formyltetrahydrofolate in mature seeds. Most of the genes involved in folate and C1 metabolism exhibited similar transcriptional expression patterns between these two maize lines, with the highest transcript abundance detected on day after pollination (DAP) 6 and the decreased transcript abundance on DAP 12 and 18. Compared with the seeds on DAP 30, 5-methyltetrahydrofolate was decreased and 5-formyltetrahydrofolate was increased sharply in the mature dry seeds. Conclusions: The enzymes involved in folate and C1 metabolism are conserved between maize and other plant species. Folate and C1 metabolism is active in young developing maize seeds at transcriptional levels.

分类号：

  • 相关文献

[1]ISOLATION AND CHARACTERIZATION OF A POLLEN-SPECIFIC GENE ZMSTK2_USP FROM ZEA MAYS. Javeed, A.,Fan, M. X.,Zhang, C. Y.,Ma, W. J.,Chen, M. M.,Liu, K. C.,Cai, F. C.,Lin, F.,Shi, L.,Hussain, K.. 2018

[2]Obesity Is Associated with Increased Red Blood Cell Folate Despite Lower Dietary Intakes and Serum Concentrations. Bird, Julia K.,Choi, Sang-Woon,Liu, Zhenhua,Ronnenberg, Alayne G.,Choi, Sang-Woon,Du, Fangling,Mason, Joel B.,Mason, Joel B.,Liu, Zhenhua.

[3]Gene Cloning and Tissue-Specific Expression of G Protein beta Subunit in Microplitis mediator (Hymenoptera: Braconidae). ZHANG Shuai,ZHANG Yong-jun,CUI Jin-jie,GAO Xi-wu,GUO Yu-yuan. 2010

[4]Molecular Analysis and Expression Patterns of Four 14-3-3 Genes from Brassica napus L.. Zhan Gao-miao,Tong Jin,Wang Han-zhong,Hua Wei. 2010

[5]Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. Li, Pan-Song,Chai, Shou-Cheng,Li, Pan-Song,Yu, Tai-Fei,He, Guan-Hua,Chen, Ming,Zhou, Yong-Bin,Xu, Zhao-Shi,Ma, You-Zhi. 2014

[6]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

[7]Characterization and Expression of Genes Encoding Three Small Heat Shock Proteins in Sesamia inferens (Lepidoptera: Noctuidae). Sun, Meng,Lu, Ming-Xing,Tang, Xiao-Tian,Du, Yu-Zhou,Sun, Meng,Lu, Ming-Xing,Tang, Xiao-Tian,Du, Yu-Zhou,Sun, Meng. 2014

[8]Molecular characterization, expression and functional analysis of NOD1, NOD2 and NLRC3 in Nile tilapia (Oreochromis niloticus). Gao, Feng-ying,Yang, Xian-le,Gao, Feng-ying,Pang, Ji-cai,Lu, Mai-xin,Zhu, Hua-ping,Ke, Xiao-li,Liu, Zhi-gang,Cao, Jian-meng,Wang, Miao,Gao, Feng-ying,Pang, Ji-cai,Lu, Mai-xin,Zhu, Hua-ping,Ke, Xiao-li,Liu, Zhi-gang,Cao, Jian-meng,Wang, Miao. 2018

[9]Expression Profiling of miRNA-122 and miRNA-221 in Porcine Various Tissues and Developing Testes. Lian, Chuanjiang,Niu, Shuling,Yang, Runjun,Sun, Boxing,Shen, Binglei,Liu, Boyang,Zhang, Liying,Zhao, Zhihui,Qin, Lihong,Bai, Wenlin. 2011

[10]Molecular Characterization of Two Sensory Neuron Membrane Proteins From Chilo suppressalis (Lepidoptera: Pyralidae). Liu, Su,Qiao, Fei,Liang, Qing-Mei,Huang, Yuan-Jie,Zhou, Wen-Wu,Gong, Zhong-Jun,Cheng, Jiaan,Zhu, Zeng-Rong,Liu, Su,Qiao, Fei,Liang, Qing-Mei,Huang, Yuan-Jie,Zhou, Wen-Wu,Gong, Zhong-Jun,Cheng, Jiaan,Zhu, Zeng-Rong,Liu, Su,Qiao, Fei,Liang, Qing-Mei,Huang, Yuan-Jie,Zhou, Wen-Wu,Gong, Zhong-Jun,Cheng, Jiaan,Zhu, Zeng-Rong,Gong, Zhong-Jun. 2013

[11]Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense. Rahman, N. M. A.,Fu, H.,Bai, H.,Jiang, F. W.,Liang, G.,Jiang, F. F.,Wu, Y.,Fu, H.,Qiao, H.,Jin, S.,Zhang, W.,Sun, S.,Gong, Y.,Xiong, Y.,Rahman, N. M. A.. 2016

[12]Genome-wide identification and expression profiling of serine proteases and homologs in the diamondback moth, Plutella xylostella (L.). Lin, Hailan,Xia, Xiaofeng,Yu, Liying,Vasseur, Liette,Gurr, Geoff M.,Yang, Guang,You, Minsheng,Lin, Hailan,Xia, Xiaofeng,Yu, Liying,Vasseur, Liette,Gurr, Geoff M.,Yang, Guang,You, Minsheng,Lin, Hailan,Yu, Liying,Lin, Hailan,Xia, Xiaofeng,Yu, Liying,Gurr, Geoff M.,Yang, Guang,You, Minsheng,Lin, Hailan,Xia, Xiaofeng,Yu, Liying,Vasseur, Liette,Gurr, Geoff M.,Yang, Guang,You, Minsheng,Vasseur, Liette,Gurr, Geoff M.,Yao, Fengluan. 2015

[13]Molecular characterization and expression analysis of a novel r-spondin member (rspo2l) in Chinese tongue sole (Cynoglossus semilaevis). Wei, Min,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Li, Hai-long. 2018

[14]Characterization and Expression Analysis of Four Glycine-Rich RNA-Binding Proteins Involved in Osmotic Response in Tobacco (Nicotiana tabacum cv. Xanthi). Zeng Qian-chun,Chen Xuan,Lu Xiu-ping,Li Wen-zheng,Yu Di-qiu. 2010

[15]Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Gu, Riliang,Zhao, Li,Zhang, Ying,Chen, Xiaoping,Bao, Juan,Zhao, Jinfeng,Wang, Zhangying,Fu, Junjie,Liu, Tingsong,Wang, Jianhua,Wang, Guoying. 2006

[16]Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Capsicum annuum L.. Diao, Wei-Ping,Wang, Shu-Bin,Liu, Jin-Bing,Pan, Bao-Gui,Guo, Guang-Jun,Wei, Ge,Diao, Wei-Ping,Snyder, John C.. 2016

[17]Cloning and expression analysis of a heat shock protein 90 beta isoform gene from the gills of Wuchang bream (Megalobrama amblycephala Yih) subjected to nitrite stress. Sun, S. M.,Zhu, J.,Ge, X. P.,Zhang, C. F.,Miao, L. H.,Jiang, X. J.. 2015

[18]Identification and expression patterns of UDP-glycosyltransferase (UGT) genes from insect pest Athetis lepigone (Lepidoptera: Noctuidae). Xu, Ji-Wei,Li, Meng-Ya,Zhu, Xiu-Yun,Ma, Ji-Fang,Dong, Zhi-Ping,Xu, Lu. 2017

[19]Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.). Zhang, Ling,Zhang, Yuan-Yu,Wang, Yu-Min,Li, Hai-Yun,Xing, Guo-Jie,Dong, Ying-Shan,Zhao, Hong-Kun,Dong, Qian-Li,Li, Qi-Yun. 2015

[20]Genome-Wide Characterization and Expression Profiling of Sugar Transporter Family in the Whitefly, Bemisia tabaci (Gennadius) (Hemiptera:Aleyrodidae). Yang, Zezhong,Zheng, Huixin,Yang, Zezhong,Xia, Jixing,Gong, Cheng,Xie, Wen,Guo, Zhaojiang,Zheng, Huixin,Yang, Xin,Wu, Qingjun,Wang, Shaoli,Zhang, Youjun,Xia, Jixing,Pan, Huipeng,Yang, Fengshan. 2017

作者其他论文 更多>>

微信小程序