Long-term High-fat High-sucrose Diet Promotes Enlarged Islets and beta-Cell Damage by Oxidative Stress in Bama Minipigs

文献类型： 外文期刊

第一作者： Zhao, Zhan-zhao

作者： Zhao, Zhan-zhao;Xin, Lei-lei;Xia, Ji-han;Yang, Shu-lin;Li, Kui;Zhao, Zhan-zhao;Chen, Yao-xing

作者机构：

关键词： Bama minipigs;pancreas;steatosis;apoptosis;oxidative stress

期刊名称：PANCREAS （ 影响因子：3.327； 五年影响因子：3.417 ）

ISSN： 0885-3177

年卷期： 2015 年 44 卷 6 期

页码：

收录情况： SCI

摘要： Objectives The effect of a long-term high-fat, high-caloric diet on the dysfunction of pancreas has not been clarified. We investigated the pancreatic histopathology and -cell apoptosis in Bama minipigs after 23 months on a high-fat high-sucrose diet (HFHSD). Methods Bama minipigs were randomly assigned to control (n = 6) and HFHSD groups (n = 6) for 23 months, and biochemical parameters were measured. Pancreata were subjected to histological analysis, followed by assessment with transmission electron microscopy. Lipid peroxidation was determined by the malondialdehyde concentration and antioxidant enzyme activity. B-cell apoptosis was measured by an immunohistochemical method. Results In the HFHSD group, the islets were enlarged, and the pancreatic tissue had observed significant fatty infiltration. Moreover, the feeding program damaged the normal pancreatic tissue structure. The level of lipid peroxidation was increased, and the activities of pancreatic antioxidant enzymes were significantly decreased. The expression levels of caspase-3, Bax, and insulin were significantly increased (P < 0.05), and the expression levels of proliferating cell nuclear antigen and Bcl-2 were decreased (P < 0.05). Conclusions The long-term HFHSD promotes pancreatic steatosis and oxidative stress, which increases -cell apoptosis as indicated by the activation of caspase-3 through the mitochondrial pathway (Bcl-2/Bax).

分类号：

  • 相关文献

[1]Oxidative Stress-Mediated Cytotoxicity of Cadmium in Chicken Splenic Lymphocytes. Li, Jin-Long,Tang, Zhao-Xin,Li, Jin-Long,Li, Shu,Xu, Shi-Wen,Li, Hui-Xin,Wang, Xiao-Long. 2010

[2]Facile fabrication of graphene oxide loaded with silver nanoparticles as antifungal materials. Cui, Jianghu,Yang, Yunhua,Zheng, Mingtao,Liu, Yingliang,Xiao, Yong,Lei, Bingfu,Chen, Wei. 2014

[3]Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells. Wang Lin,Liu Gang,Wang Zhen Yong,Lin Shu Qian,He Yuan Long. 2013

[4]Toxic dinoflagellate Alexandrium tamarense induces oxidative stress and apoptosis in hepatopancreas of shrimp (Fenneropenaeus chinensis). Liang Zhongxiu,Ren Hai,Liang Zhongxiu,Li Jian,Li Jitao,Tan Zhijun,Zhao Fazhen. 2014

[5]Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Jia, Rui,Huo, Huan-Huan,Jia, Rui,Lei, Ji-Lin,Liu, Bao-Liang,Huang, Bin,Yin, Shu-Ting,Han, Cen.

[6]Rhizoma smilacis glabrae protects rats with gentamicin-induced kidney injury from oxidative stress-induced apoptosis by inhibiting caspase-3 activation. Liu, Cuiyan,Kang, Youxi,Yang, Zisheng,Han, Chunyang,Zhou, Xiuhong,Gu, Jingang.

[7]Molecular cloning, immunohistochemical localization, characterization and expression analysis of caspase-8 from the blunt snout bream (Megalobrama amblycephala) exposed to ammonia. Sun, Shengming,Ge, Xianping,Zhu, Jian,Zhang, Wuxiao,Zhang, Qiong.

[8]Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress. Cheng, Chang-Hong,Guo, Zhi-Xun,Ye, Chao-Xia,Wang, An-Li,Guo, Zhi-Xun.

[9]Cytoprotective effect of deferiprone against aluminum chloride-induced oxidative stress and apoptosis in lymphocytes. Zhuang, Cuicui,Zhang, Haiyang,Song, Miao,Han, Yanfei,Li, Yanfei,She, Yue,Zhu, Yanzhu. 2018

[10]Protective Effects of Cynaroside Against H2O2-Induced Apoptosis in H9c2 Cardiomyoblasts. Sun, Xiao-bo,Sun, Xiao-bo. 2011

[11]Carbendazim has the potential to induce oxidative stress, apoptosis, immunotoxicity and endocrine disruption during zebrafish larvae development. Jiang, Jinhua. 2015

[12]Pretilachlor has the potential to induce endocrine disruption, oxidative stress, apoptosis and immunotoxicity during zebrafish embryo development. Jiang, Jinhua,Chen, Yanhong,Yu, Ruixian,Zhao, Xueping,Wang, Qiang,Cai, Leiming. 2016

[13]An isoflavonoid-enriched extract from Pueraria lobata (kudzu) root protects human umbilical vein endothelial cells against oxidative stress induced apoptosis. Gao, Ying,Gao, Ying,Wang, Xu,He, Chunnian.

[14]X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets. Cai, Xuan,Zhu, Lihui,Sheng, Yongshuai,Guo, Qi,Bao, Jian,Xu, Jianxiong,Cai, Xuan,Zhu, Lihui,Sheng, Yongshuai,Guo, Qi,Bao, Jian,Xu, Jianxiong,Chen, Xiaolian.

[15]Neuroprotection against hydrogen peroxide-induced toxicity by Dictyophora echinovolvata polysaccharide via inhibiting the mitochondria-dependent apoptotic pathway. Yu, Wen-Xuan,Dong, Xiao-Li,Lin, Chen-Qiang,Lin, Xin-Jian,Yu, Wen-Xuan,Dong, Xiao-Li,Yu, Wen-Xuan,Dong, Xiao-Li,Zhao, Qing.

[16]Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish. Yin, Guojun,Kitazawa, Takio,Teraoka, Hiroki,Yin, Guojun,Cao, Liping,Du, Jinliang,Jia, Rui,Kubota, Akira.

[17]Effects of trans-10, cis-12-conjugated linoleic acid on mice are influenced by the dietary fat content and the degree of murine obesity. Li, Shi-Li,Li, Mei-Juan,Gou, Ke-Mian,Ma, Shu-Yu,Fan, Zhi-Ying,Xu, Bin-Rui,Cao, Wen-Guang.

[18]Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets. Tian, Zhi-mei,Ma, Xian-yong,Yang, Xue-fen,Fan, Qiu-li,Xiong, Yun-xia,Qiu, Yue-qin,Wang, Li,Wen, Xiao-lu,Jiang, Zong-yong. 2016

[19]Preparation of peptone from chicken bone residue by using natural pancreas as catalyst. Wang, Jin-Zhi,Yue, Jian-Ying,Zhang, Chun-Hui,Jia, Wei,Li, Xia,Sun, Zhen.

[20]Effects of selenium and sulfur on antioxidants and physiological parameters of garlic plants during senescence. Cheng Bo,Lian Hai-feng,Yu Xin-hui,Sun Ya-li,Sun Xiu-dong,Shi Qing-hua,Liu Shi-qi,Liu Ying-ying. 2016

作者其他论文 更多>>
  • EXPERT expands prime editing efficiency and range of large fragment edits

    作者：Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Han, Xiaosong;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Li, Kui;Zhang, Jifeng;Xu, Jie

    关键词：

  • Genome-Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells

    作者：Zhu, Xiusheng;Huang, Lei;Li, Guoli;Deng, Biao;Wang, Xiaoxiao;Yang, Hu;Zhang, Yuanyuan;Wen, Qiuhan;Wang, Chao;Zhang, Jingshu;Li, Kui;Liu, Yuwen;Deng, Biao;Deng, Biao;Zhao, Yunxiang

    关键词：cis-regulatory elements; high-throughput; iPSCs; silencer; Ss-STARR-seq

  • Transcriptome analysis reveals no obvious unintended effects in the spleen of CRISPR/Cas9-mediated CD163 and pAPN double-knockout pigs

    作者：Liu, Zhi-Guo;Wang, Nan;Wang, Wan-Jie;Chi, Shun-Shun;Nie, Yu-Xin;Yuan, Mao-Sha;Sun, Ya-Ru;Mu, Yu-Lian;Li, Kui;Wang, Nan;Huang, Lei;Chi, Shun-Shun;Li, Kui

    关键词：CD163; pAPN; Double-knockout; Gene editing; Pig; Spleen; Unintended effects; Transcriptomics

  • Integrating large-scale meta-GWAS and PigGTEx resources to decipher the genetic basis of 232 complex traits in pigs

    作者：Xu, Zhiting;Lin, Qing;Cai, Xiaodian;Zhong, Zhanming;Teng, Jinyan;Zeng, Haonan;Gao, Yahui;Wei, Chen;Zhou, Jun;Zhang, Wenjing;Zhang, Xiaoke;Shi, Shaolei;Wu, Jun;Diao, Shuqi;Liu, Yuqiang;Pan, Xiangchun;Feng, Xueyan;Liu, Ruiqi;Su, Zhanqin;Chang, Chengjie;Zhu, Qianghui;Wu, Yuwei;Zhang, Hao;Yuan, Xiaolong;Li, Jiaqi;Zhang, Zhe;Li, Bingjie;Gao, Yahui;Liu, George E.;Gao, Yahui;Ma, Li;Cai, Zexi;Bai, Zhonghao;Su, Guosheng;Sahana, Goutam;Lund, Mogens Sando;Fang, Lingzhao;Wang, Xiaoqing;Shi, Liangyu;Zhao, Fuping;Wang, Xue;Wang, Yi;Zhang, Zipeng;Ding, Xiangdong;Lin, Yu;Tang, Guoqing;Li, Mingzhou;Li, Xuewei;Liu, Shuli;Yin, Hongwei;Bai, Lijing;Li, Kui;Zhou, Zhongyin;Wang, Qishan;Pan, Yuchun;Xu, Zhong;Peng, Xianwen;Mei, Shuqi;Mo, Delin;Liu, Xiaohong;Chen, Yaosheng;Liu, Yang;Xiang, Ruidong;Xiang, Ruidong;Shen, Xia;Shen, Xia;Shen, Xia;Li, Pinghua;Huang, Ruihua;Li, Pinghua;Huang, Ruihua;Ballester, Maria;Crespo-Piazuelo, Daniel;Amills, Marcel;Clop, Alex;Amills, Marcel;Karlskov-Mortensen, Peter;Fredholm, Merete;Zhang, Qin;Zhao, Yunxiang

    关键词：pig; meta-GWAS; PigGTEx; molQTL; complex traits

  • Multi-dimensional annotation of porcine variants using genomic and epigenomic features in pigs

    作者：Yin, Hongwei;Yang, Liu;Zhao, Qianyi;Yao, Wenye;Bai, Lijing;Li, Kui;Fang, Lingzhao;Yao, Wenye;Groenen, Martien A. M.;Madsen, Ole;Teng, Jinyan;Gao, Yahui;Xu, Zhiting;Lin, Qing;Diao, Shuqi;Li, Jiaqi;Zhang, Zhe;Zhou, Huaijun;Guan, Dailu;Liu, Xiaohong;Zhao, Fuping;Zhou, Zhongyin;Wang, Qishan

    关键词：Genomic variants; Gene expression and splicing; Complex traits; Non-coding regions; Epigenome

  • DPImpute: A Genotype Imputation Framework for Ultra-Low Coverage Whole-Genome Sequencing and its Application in Genomic Selection

    作者：Zheng, Weigang;Wang, Chao;Dong, Wenjun;Liu, Yuwen;Zheng, Weigang;Wang, Chao;Dong, Wenjun;Liu, Yuwen;Zheng, Weigang;Ma, Wenlong;Chen, Zhilong;Wang, Chao;Sun, Tao;Dong, Wenjun;Zhang, Song;Tang, Zhonglin;Li, Kui;Liu, Yuwen;Zheng, Weigang;Ma, Wenlong;Chen, Zhilong;Wang, Chao;Sun, Tao;Dong, Wenjun;Zhang, Song;Tang, Zhonglin;Li, Kui;Liu, Yuwen;Zhang, Wenjing;Tang, Zhonglin;Liu, Yuwen;Zhao, Yunxiang

    关键词：breeding models; genetic screening of embryos; genomic selection; genotype imputation; single cell genotype

  • Innovation in Agricultural Water Pricing Systems in China Based on Irrigation Benefits

    作者：Feng, Xin;Li, Kui;Feng, Xin;Liu, Zixuan;Jiang, Wenlai;Liu, Yang

    关键词：irrigation benefit sharing coefficient; grain crops; C-D production function; sharing of agricultural water prices; sustainable agricultural development

微信小程序