Functional Characterization of New Polyketide Synthase Genes Involved in Ochratoxin A Biosynthesis in Aspergillus Ochraceus fc-1

文献类型： 外文期刊

第一作者： Wang, Liuqing

作者： Wang, Liuqing;Wang, Yan;Wang, Qi;Liu, Fei;Selvaraj, Jonathan Nimal;Liu, Lingna;Xing, Fuguo;Zhao, Yueju;Zhou, Lu;Liu, Yang;Wang, Yan;Xing, Fuguo;Zhao, Yueju;Zhou, Lu;Liu, Yang

作者机构：

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2015 年 7 卷 8 期

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA), a potentially carcinogenic mycotoxin which contaminates grains, is produced by several Aspergillus species. A comparative sequence analysis of the OTA-producing Aspergillus ochraceus fc-1 strain and other Aspergillus species was performed. Two new OTA-related polyketide synthase (PKS) (AoOTApks) genes were identified. The predicted amino acid sequence of AoOTApks-1 displayed high similarity to previously identified PKSs from OTA-producing A. carbonarius ITEM 5010 (67%; [PI] No. 173482) and A. niger CBS 513.88 (62%; XP_001397313). However, the predicted amino acid sequence of AoOTApks-2 displayed lower homology with A. niger CBS 513.88 (38%) and A. carbonarius ITEM 5010 (28%). A phylogenetic analysis of the beta-ketosynthase and acyl-transferase domains of the AoOTApks proteins indicated that they shared a common origin with other OTA-producing species, such as A. carbonarius, A. niger, and A. westerdijkiae. A real-time reverse-transcription PCR analysis showed that the expression of AoOTApks-1 and -2 was positively correlated with the OTA concentration. The pks gene deleted mutants increment AoOTApks-1 and increment AoOTApks-2 produced nil and lesser OTA than the wild-type strain, respectively. Our study suggests that AoOTApks-1 could be involved in OTA biosynthesis, while AoOTApks-2 might be indirectly involved in OTA production.

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