Selection and evaluation of reference genes for quantitative gene expression studies in cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

文献类型： 外文期刊

第一作者： Shakeel, Muhammad

作者： Shakeel, Muhammad;Zhu, Xun;Kang, Tinghao;Wan, Hu;Li, Jianhong;Zhu, Xun

作者机构：

关键词： Helicovespa armigero;qRT-PCR analysis;Reference gene;geNorm;NormFinder;BestKeeper

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2015 年 18 卷 2 期

页码：

收录情况： SCI

摘要： An efficient technique for investigating gene expression is the real time quantitative reverse transcription PCR (qRT-PCR). Despite the fact that this technique has been extensively used to explore the gene function in Helicoverpa armigera, stability of the reference genes still requires validation. This research aims to validate the stability of expression of nine potential reference genes under different experimental conditions including temperature, mechanical injury, starvation, photoperiod, and developmental stage. An exhaustive system (RefFinder), available online, was employed to evaluate and grade the studied genes. Appropriateness of the reference genes as endogenous controls was determined through four computational algorithms (Delta Ct, NormFinder, BestKeeper, and geNorm). According to the findings of this study, RPL28 and RPS15 were found to be the most stable reference genes in case of starved larvae, temperature stressed larvae; and different developmental stages. On the other hand, HSP90 and TUBB proved to be highly stable in case of photoperiod stressed larvae. Finally, TUBB and GAPDH were the most stable reference genes in case of larvae subjected to mechanical injury. These results can facilitate development of a standardized qRT-PCR technique and can also prove to be helpful for standard RT-PCR method which need reference gene for normalization. (C) 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Identification and testing of reference genes for Sesame gene expression analysis by quantitative real-time PCR. Wei, Libin,Miao, Hongmei,Zhao, Ruihong,Han, Xiuhua,Zhang, Tide,Zhang, Haiyang.

[2]Identification of reference genes for reverse transcription quantitative real-time PCR normalization in pepper (Capsicum annuum L.). Wan, Hongjian,Yuan, Wei,Ruan, Meiying,Ye, Qingjing,Wang, Rongqing,Li, Zhimiao,Zhou, Guozhi,Yao, Zhuping,Zhao, Jing,Liu, Shujun,Yang, Yuejian. 2011

[3]Identification of Reference Genes for Studying Herbicide Resistance Mechanisms in Japanese Foxtail (Alopecurus japonicus). Xu, Hongle,Li, Jun,Wu, Xibao,Wang, Lingyue,Dong, Liyao,Xu, Hongle,Wu, Renhai,Su, Wangcang.

[4]Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz. Yi, Shanjun,Qian, Yongqiang,Han, Lei,Sun, Zhenyuan,Liu, Junxiang,Ju, Guansheng,Fan, Chengming.

[5]Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time PCR. Hu, Meizhen,Wang, Wenquan,Hu, Meizhen,Xia, Zhiqiang,Zhou, Xincheng,Wang, Wenquan,Hu, Meizhen,Wang, Wenquan,Hu, Wenbin. 2016

[6]In-depth analysis of internal control genes for quantitative real-time PCR in Brassica oleracea var. botrytis. Sheng, X. G.,Zhao, Z. Q.,Yu, H. F.,Wang, J. S.,Gu, H. H.,Zheng, C. F.. 2016

[7]Exploring valid reference genes for quantitative real-time PCR analysis in Plutella xylostella (Lepidoptera: Plutellidae). Zhou, Xiaomao,Fu, Wei,Xie, Wen,Wang, Shaoli,Wu, Qingjun,Zhang, Youjun,Fu, Wei,Zhang, Zhuo,Liu, Yong,Zhang, Zhuo,Liu, Yong,Zhou, Xuguo.

[8]Reference gene selection for quantitative real-time PCR normalization in different cherry genotypes, developmental stages and organs. Ye, Xia,Zhang, Fangming,Tao, Yonghuan,Song, Shangwei,Fang, Jinbao.

[9]Comprehensive evaluation of candidate reference genes for gene expression studies in Lysiphlebia japonica (Hymenoptera: Aphidiidae) using RT-qPCR. Xue-Ke Gao,Cui, Jin-Jie,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min Lü,Li-Juan Zhang,Xiang-Zhen Zhu,Li Wang,Hui Lu,Jin-Jie Cui.

[10]Selection of reference genes for studies of porcine endometrial gene expression on gestational day 12. Zhang, Hao. 2011

[11]Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs. Niu, Guanglin,Yang, Yalan,Zhang, YuanYuan,Hua, Chaoju,Wang, Zishuai,Tang, Zhonglin,Li, Kui,Niu, Guanglin,Yang, Yalan,Tang, Zhonglin,Li, Kui. 2016

[12]Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole (Cynoglossus semilaevis). Ma Qian,Zhuang Zhimeng,Feng Wenrong,Liu Shufang,Tang Qisheng. 2015

[13]Validation of Reference Genes for Quantitative Real-Time PCR during Bicolor Tepal Development in Asiatic Hybrid Lilies (Lilium spp.). Xu, Leifeng,Xu, Hua,Cao, Yuwei,Yang, Panpan,Feng, Yayan,Tang, Yuchao,Yuan, Suxia,Ming, Jun,Yang, Panpan. 2017

[14]Development and application of a general plasmid reference material for GMO screening. Wu, Yuhua,Li, Jun,Wang, Yulei,Li, Xiaofei,Li, Yunjing,Zhu, Li,Li, Jun,Wu, Gang,Wu, Yuhua,Li, Jun,Wang, Yulei,Li, Xiaofei,Li, Yunjing,Zhu, Li,Li, Jun,Wu, Gang. 2016

[15]Selection of Reference Genes for Expression Analysis of Kumamoto and Portuguese Oysters and Their Hybrid. Yan Lulu,Wang Zhaoping,Yu Ruihai,Yan Lulu,Su Jiaqi,Yan Xiwu. 2017

[16]Selection of reference genes for quantitative real-time PCR in Cocos nucifera during abiotic stress. Xia, Wei,Liu, Zheng,Yang, Yaodong,Xiao, Yong,Zhao, Songlin,Mason, Annaliese S.,Mason, Annaliese S.,Ma, Zilong. 2014

[17]Selection of reliable reference genes for gene expression studies in Clonostachys rosea 67-1 under sclerotial induction. Li, Shi-Dong,Sun, Man-Hong.

[18]Analysis of multiple transcriptomes of the African oil palm (Elaeis guineensis) to identify reference genes for RT-qPCR. Xia, Wei,Xiao, Yong,Liu, Zheng,Yang, Yaodong,Lei, Xintao,Ma, Zilong,Peng, Ming,Mason, Annaliese S.,Mason, Annaliese S.,Wu, Xiaoming.

[19]Reference genes selection for transcript normalization in kenaf (Hibiscus cannabinus L.) under salinity and drought stress. Niu, Xiaoping,Qi, Jianmin,Chen, Meixia,Tao, Aifen,Fang, Pingping,Xu, Jiantang,Onyedinma, Sandra A.,Chen, Meixia,Zhang, Gaoyang,Su, Jianguang. 2015

[20]Identification of suitable reference genes during the formation of chlamydospores in Clonostachys rosea 67-1. Sun, Zhanbin,Li, Shidong,Sun, Manhong. 2017

作者其他论文 更多>>

微信小程序