The Yeast GSK-3 Homologue Mck1 Is a Key Controller of Quiescence Entry and Chronological Lifespan

文献类型： 外文期刊

第一作者： Quan, Zhenzhen

作者： Quan, Zhenzhen;Cao, Lu;Tang, Yingzhi;Oliver, Stephen G.;Zhang, Nianshu;Quan, Zhenzhen;Cao, Lu;Tang, Yingzhi;Oliver, Stephen G.;Zhang, Nianshu;Yan, Yanchun

作者机构：

期刊名称：PLOS GENETICS （ 影响因子：5.917； 五年影响因子：6.284 ）

ISSN： 1553-7390

年卷期： 2015 年 11 卷 6 期

页码：

收录情况： SCI

摘要： Upon starvation for glucose or any other core nutrient, yeast cells exit from the mitotic cell cycle and acquire a set of G(0)-specific characteristics to ensure long-term survival. It is not well understood whether or how cell cycle progression is coordinated with the acquisition of different G(0)-related features during the transition to stationary phase (SP). Here, we identify the yeast GSK-3 homologue Mck1 as a key regulator of G(0) entry and reveal that Mck1 acts in parallel to Rim15 to activate starvation-induced gene expression, the acquisition of stress resistance, the accumulation of storage carbohydrates, the ability of early SP cells to exit from quiescence, and their chronological lifespan. FACS and microscopy imaging analyses indicate that Mck1 promotes mother-daughter cell separation and together with Rim15, modulates cell size. This indicates that the two kinases coordinate the transition-phase cell cycle, cell size and the acquisition of different G(0)-specific features. Epistasis experiments place MCK1, like RIM15, downstream of RAS2 in antagonising cell growth and activating stress resistance and glycogen accumulation. Remarkably, in the ras2 Delta cells, deletion of MCK1 and RIM15 together, compared to removal of either of them alone, compromises respiratory growth and enhances heat tolerance and glycogen accumulation. Our data indicate that the nutrient sensor Ras2 may prevent the acquisition of G(0)-specific features via at least two pathways. One involves the negative regulation of the effectors of G(0) entry such as Mck1 and Rim15, while the other likely to involve its functions in promoting respiratory growth, a phenotype also contributed by Mck1 and Rim15.

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