iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes

文献类型: 外文期刊

第一作者: Zhang, Ji-ze

作者: Zhang, Ji-ze;Gao, Yang;Lu, Qing-ping;Sa, Ren-na;Zhang, Hong-fu;Gao, Yang

作者机构:

关键词: Non-starch polysaccharide enzymes (NSPEs);Longissimus muscle;Proteomics;Growing pigs

期刊名称:JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B ( 影响因子:3.066; 五年影响因子:3.057 )

ISSN: 1673-1581

年卷期: 2015 年 16 卷 6 期

页码:

收录情况: SCI

摘要: Non-starch polysaccharide enzymes (NSPEs) have long been used in the feed production of monogastric animals to degrade non-starch polysaccharide to oligosaccharides and promote growth performance. However, few studies have been conducted on the effect of such enzymes on skeletal muscle in monogastric animals. To elucidate the mechanism of the effect of NSPEs on skeletal muscle, an isobaric tag for relative and absolute quantification (iTRAQ) for differential proteomic quantitation was applied to investigate alterations in the proteome in the longissimus muscle (LM) of growing pigs after a 50-d period of supplementation with 0.6% NSPEs in the diet. A total of 51 proteins were found to be differentially expressed in the LM between a control group and the NSPE group. Functional analysis of the differentially expressed protein species showed an increased abundance of proteins related to energy production, protein synthesis, muscular differentiation, immunity, oxidation resistance and detoxification, and a decreased abundance of proteins related to inflammation in the LM of the pigs fed NSPEs. These findings have important implications for understanding the mechanisms whereby dietary supplementation with NSPEs enzymes can promote growth performance and improve muscular metabolism in growing pigs.

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