Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis

文献类型： 外文期刊

第一作者： Peng, Qi

作者： Peng, Qi;Wang, Guannan;Zhang, Jie;Song, Fuping;Liu, Guiming

作者机构：

关键词： Bacillus thuringiensis;sigma(54);bEBPs;metabolic pathways;DNA microarray

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2015 年 6 卷

页码：

收录情况： SCI

摘要： Sigma(54) (sigma(54)) regulates nitrogen and carbon utilization in bacteria. Promoters that are sigma(54)-dependent are highly conserved and contain short sequences located at the -24 and -12 positions upstream of the transcription initiation site. sigma(54) requires regulatory proteins known as bacterial enhancer-binding proteins (bEBPs) to activate gene transcription. We show that sigma(54) regulates the capacity to grow on various nitrogen sources using a Bacillus thuringiensis HD73 mutant lacking the sigL gene encoding sigma(54) (Delta sigL). A 2-fold-change cutoff and a false discovery rate cutoff of P < 0.05 were used to analyze the DNA microarray data, which revealed 255 genes that were downregulated and 121 that were upregulated in the Delta sigL mutant relative to the wild-type HD73 strain. The sigma(54) (stationary phase) was characterized by DNA microarray, bioinformatics, and functional assay; 16 operons containing 47 genes were identified whose promoter regions contain the conserved -12/-24 element and whose transcriptional activities were abolished or reduced in the Delta sigL mutant. Eight sigma(54)-dependent transcriptional bEBPs were found in the Bt HD73 genome, and they regulated nine sigma(54)-dependent promoters. The metabolic pathways activated by sigma(54) this process have yet to be identified in Bacillus thuringiensis; nonetheless, the present analysis of the sigma(54) regulon provides a better understanding of the physiological roles of sigma factors in bacteria.

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