Generation of peanut mutants by fast neutron irradiation combined with in vitro culture

文献类型： 外文期刊

第一作者： Wang, Jing-Shan

作者： Wang, Jing-Shan;Sui, Jiong-Ming;Qiao, Li-Xian;Xie, Yong-Dun;Guo, Hui-Jun;Liu, Lu-Xiang;Zhao, Li-Lan;Yu, Shan-Lin

作者机构：

关键词： peanut (Arachis hypogaea L.);fast neutron irradiation;mutagenesis;in vitro culture;mutant

期刊名称：JOURNAL OF RADIATION RESEARCH （ 影响因子：2.724； 五年影响因子：2.587 ）

ISSN： 0449-3060

年卷期： 2015 年 56 卷 3 期

页码：

收录情况： SCI

摘要： Induced mutations have played an important role in the development of new plant varieties. In this study, we investigated the effects of fast neutron irradiation on somatic embryogenesis combined with plant regeneration in embryonic leaflet culture to develop new peanut (Arachis hypogaea L.) germplasm for breeding. The dry seeds of the elite cultivar Luhua 11 were irradiated with fast neutrons at dosages of 9.7, 14.0 and 18.0 Gy. The embryonic leaflets were separated and incubated in a medium with 10.0-mg/l 2,4-D to induce somatic embryogenesis. Next, they were incubated in a medium with 4.0-mg/l BAP for plant regeneration. As the irradiation dosage increased, the frequency of both somatic embryo formation and plantlet regeneration decreased. The regenerated plantlets were grafted onto rootstocks and were transplanted into the field. Later, the mature seeds of the regenerated plants were harvested. The M-2 generation plants from most of the regenerated cultivars exhibited variations and segregation in vigor, plant height, branch and pod number, pod size, and pod shape. To determine whether the phenotypes were associated with genomic modification, we compared the DNA polymorphisms between the wild-type plants and 19 M-3-generation individuals from different regenerated plants. We used 20 pairs of simple sequence repeat (SSR) primers and detected polymorphisms between most of the mutants and the wild-type plants (Luhua 11). Our results indicate that using a combination of fast neutron irradiation and tissue culture is an effective approach for creating new peanut germplasm.

分类号：

  • 相关文献

[1]Transcriptome profiling of peanut gynophores revealed global reprogramming of gene expression during early pod development in darkness. Xia, Han,Zhao, Chuanzhi,Hou, Lei,Li, Aiqin,Zhao, Shuzhen,Bi, Yuping,An, Jing,Wan, Shubo,Wang, Xingjun,Bi, Yuping,Wan, Shubo,Wang, Xingjun,Bi, Yuping,An, Jing,Zhao, Yanxiu,Wang, Xingjun. 2013

[2]High efficiency in vitro plant regeneration from epicotyl explants of Chinese peanut cultivars. Shan, Lei,Tang, Guiying,Xu, Pingli,Liu, Zhanji,Bi, Yuping,Shan, Lei,Tang, Guiying,Xu, Pingli,Liu, Zhanji,Bi, Yuping,Shan, Lei,Tang, Guiying,Xu, Pingli,Liu, Zhanji,Bi, Yuping.

[3]Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.). Yu, Shan-Lin,Sui, Jiong-Ming,Li, Rui,Fan, Qian-Cheng,Zheng, Chun-Hua,Wang, Jing-Shan,Qiao, Li-Xian,Song, Lin. 2013

[4]Modified AnM technique in combination with black and transparent film mulching in peanut production. Qin, Feifei,Takano, Tetsuo,Qin, Feifei,Xu, Hui-lian,Qin, Feifei. 2012

[5]Metabolic changes of peanut (Arachis hypogaea L.) buds in response to low temperature (LT). Wang, X. J.,Sun, D. L.,Bian, N. F.,Zhang, Z. M.,Wang, X.,Xu, Z. J.,Qi, Y. J.,Shen, Y..

[6]Peanut violaxanthin de-epoxidase alleviates the sensitivity of PSII photoinhibition to heat and high irradiance stress in transgenic tobacco. Yang, Sha,Meng, De-Yun,Hou, Lin-Lin,Li, Yan,Guo, Feng,Meng, Jing-Jing,Li, Xin-Guo,Wan, Shu-Bo,Meng, De-Yun,Hou, Lin-Lin.

[7]Mutant Transcriptome Sequencing Provides Insights into Pod Development in Peanut (Arachis hypogaea L.). Wan, Liyun,Li, Bei,Lei, Yong,Yan, Liying,Ren, Xiaoping,Chen, Yuning,Jiang, Huifang,Zhang, Juncheng,Liao, Boshou,Dai, Xiaofeng,Guo, Wei,Chen, Ao. 2017

[8]Quantitative trait locus analysis for pod- and kernel-related traits in the cultivated peanut (Arachis hypogaea L.). Chen, Weigang,Jiao, Yongqing,Cheng, Liangqiang,Huang, Li,Liao, Boshou,Tang, Mei,Ren, Xiaoping,Zhou, Xiaojing,Chen, Yuning,Jiang, Huifang. 2016

[9]Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.). Chen, Na,Chi, Xiaoyuan,Zhang, Zhimeng,Pan, Lijuan,Chen, Mingna,Wang, Tong,Wang, Mian,Yang, Zhen,Yu, Shanlin,Su, Maowen,Chi, Xiaoyuan.

[10]In vitro mutagenesis and directed screening for salt-tolerant mutants in peanut. Zhao, Ming-Xia,Sun, Hai-Yan,Ji, Rui-Rui,Sui, Jiong-Ming,Qiao, Li-Xian,Wang, Jing-Shan,Hu, Xiao-Hui,Chen, Jing.

[11]Genome shuffling of Lactobacillus plantarum C88 improves adhesion. Zhao, Yujuan,Duan, Cuicui,Gao, Lei,Yu, Xue,Niu, Chunhua,Li, Shengyu. 2017

[12]Molecular biological effect of (CO)-C-60 gamma-ray irradiation on rice genome DNA. Xiang, TH,Yang, JB,Yang, QJ,Zhu, QS,Li, L,Ni, DH,Wang, XF,Huang, DN. 2002

[13]The pqqC gene is essential for antifungal activity of Pseudomonas kilonensis JX22 against Fusarium oxysporum f. sp lycopersici. Xu, Jianhong,Xu, Jianhong,Deng, Peng,Baird, Sonya M.,Lu, Shi-En,Showmaker, Kurt C.,Wang, Hui. 2014

[14]Structural and biochemical insights into the degradation mechanism of chitosan by chitosanase OU01. Lyu, Qianqian,Yang, Yan,Han, Baoqin,Liu, Wanshun,Liu, Weizhi,Shi, Yanhong,Wang, Song,Jones, David N. M..

[15]Genome Editing in Cotton with the CRISPR/Cas9 System. Wei Gao,Lu Long,Song, Chunpeng,Xinquan Tian,Ji Liu,Prashant K. Singh,Jose R. Botella,Chunpeng Song. 2017

[16]Insights into the roles of non-catalytic residues in the active site of a GH10 xylanase with activity on cellulose. Chu, Yindi,Tu, Tao,Xue, Xianli,Wang, Xiaoyu,Gong, Li,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Penttinen, Leena,Rouvinen, Juha,Hakulinen, Nina,Yi, Zhuolin,Gong, Li. 2017

[17]A semi-rational design strategy of directed evolution combined with chemical synthesis of DNA sequences. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Gao, Feng,Xu, Fang,Cai, Bin,Yao, Quan-Hong.

[18]Cloning, Mutagenesis and Expression of the Housefly Acetylcholinesterase in Pichia pastoris, and its Potential Application in the Detection of Pesticide Residues. Tan, Furong,Wang, Ligang,Wang, Jinbin,Wu, Xiao,Zhu, Hong,Zhao, Kai,Jiang, Lingxi,Tao, Shiru,Tang, Xueming,Tan, Furong,Wang, Ligang,Wang, Jinbin,Wu, Xiao,Zhu, Hong,Zhao, Kai,Jiang, Lingxi,Tao, Shiru,Tang, Xueming,Yang, Yan. 2010

[19]Heavy genetic load associated with the subspecific differentiation of japonica rice (Oryza sativa ssp japonica L.). Xu, Jian-Long,Wang, Jun-Min,Sun, Ye-Qing,Wei, Li-Jun,Luo, Rong-Ting,Zhang, Ming-Xian,Li, Zhi-Kang. 2006

[20]The Fate of Arabidopsis thaliana Homeologous CNSs and Their Motifs in the Paleohexaploid Brassica rapa. Subramaniam, Sabarinath,Freeling, Michael,Wang, Xiaowu,Pires, J. Chris. 2013

作者其他论文 更多>>

微信小程序