Purification and molecular docking study of a novel angiotensin-I converting enzyme (ACE) inhibitory peptide from alcalase hydrolysate of ultrasonic-pretreated silkworm pupa (Bombyx mori) protein
文献类型: 外文期刊
第一作者: Jia, Junqiang
作者: Jia, Junqiang;Wu, Qiongying;Yan, Hui;Gui, Zhongzheng;Jia, Junqiang;Wu, Qiongying;Yan, Hui;Gui, Zhongzheng
作者机构:
关键词: Silkworm pupa (Bombyx mori);Ultrasonic pretreatment;ACE inhibitory peptide;Purification;Molecular docking
期刊名称:PROCESS BIOCHEMISTRY ( 影响因子:3.757; 五年影响因子:3.665 )
ISSN: 1359-5113
年卷期: 2015 年 50 卷 5 期
页码:
收录情况: SCI
摘要: Silkworm pupa (Bombyx mori) protein (SPP) was treated by ultrasound, and then was hydrolyzed using alcalase. The hydrolysate with the highest ACE inhibitory activity was obtained at hydrolysis of 50 min when SPP was treated at power of 410W/100 ml for 32 min. The hydrolysate was fractionated by ultrafiltration, and peptide with the highest ACE inhibitory activity was purified from <5 kDa fraction using gel filtration and RP-HPLC. A novel peptide was identified as Lys-His-Val (IC50 = 12.82 mu M), and it was stable against the gastrointestinal proteases. The molecular docking study revealed that ACE inhibitory activity of the tripeptide was mainly attributed to the hydrogen bond interactions and Zn(II) interaction between the tripeptide and ACE. These results suggest that the tripeptide is a potential natural ACE inhibitor that can be used as drug or functional food ingredient. (C) 2015 Elsevier Ltd. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Hedgerow-grass ditch system effectively reduces sediment yield and nitrogen loss with surface runoff during simulated rainfall
作者:Zhao, Xinmei;Li, Tianyang;Song, Lan;Li, Yonghao;He, Binghui;Zhao, Xinmei;Zhu, Hongye;Wang, Chi;Yan, Hui
关键词:Hedgerow; Drainage ditches; N losses; Non-point source; Sloping farmland
-
An Antibacterial Hydrogel Based on Silk Sericin Cross-Linking Glycyrrhizic Acid and Silver for Infectious Wound Healing
作者:Li, Xiang;Li, Yurong;Tehoungue, Akoumay;Yan, Hui;Zhang, Guozheng;Zhang, Yeshun;Li, Yurong;Yan, Hui;Zhang, Guozheng;Zhang, Yeshun;Wang, Qianyan
关键词:
-
The E3 ubiquitin ligase SCFEDL3 mediates salt stress response by degradation of IbPP2CA2 to regulate ABA signaling in sweetpotato
作者:Wang, Dandan;Li, Chengyang;Song, Weihan;Tang, Wei;Chen, Yuyu;Kou, Meng;Gao, Runfei;Gao, Tianqi;Li, Chen;Yan, Hui;Zhang, Aicen;Wang, Xin;Zhang, Yungang;Li, Qiang
关键词:
EDL3 ; sweetpotato; PP2CA; ubiquitination; ABA signaling; salt stress response -
A multifunctional sericin hydrogel system fabricated via one-step acetic acid induction for wound management
作者:Li, Yurong;Wei, Yongkang;Gao, Zilong;Zhang, Guozheng;Zhu, Mingkun;Yan, Hui;Zhang, Yeshun;Li, Yurong;Zhang, Guozheng;Zhu, Mingkun;Yan, Hui;Zhang, Yeshun
关键词:
-
The IbMYB52/IbARF11L-IbDRM1 module negatively regulates the root development of sweetpotato
作者:Li, Chengyang;Song, Weihan;Wang, Dandan;Li, Chen;Tang, Wei;Gao, Runfei;Zhang, Aicen;Gao, Tianqi;Yan, Hui;Wang, Xin;Kou, Meng;Zhang, Yungang;Li, Qiang
关键词:Sweetpotato; IbDRM1; Roots development; IbMYB52; IbARF11L; Auxin
-
Interpretable AI-driven multidimensional chemical fingerprints for geographical authentication of Euryales Semen
作者:Yu, Daixin;Qu, Cheng;Wen, Pei;Yan, Hui;Wu, Qinan;Nie, Jing;Yuan, Yuwei;Zhao, Yuyang;Dai, Caiyan
关键词:
-
Genomic and transcriptomic insights into the cellulose-degrading mechanism of Bacillus subtilis DC-11 and its novel cellulose catabolic pathway
作者:Chen, Chen;Zhang, Minqi;Zhang, Yuanhao;Jiang, Xueping;Kong, Jia;Zhou, Jieling;Zhang, Ran;Li, Hao;Gui, Zhongzheng;Zhang, Ran;Li, Hao;Gui, Zhongzheng;Huang, Gaiqun
关键词:Silkworm excrement;
Bacillus subtilis ; Genomics; Transcriptome; Catabolic pathway