Functional characterization of a cystatin from the tick Rhipicephalus haemaphysaloides

文献类型： 外文期刊

第一作者： Wang, Yujian

作者： Wang, Yujian;Zhou, Yongzhi;Gong, Haiyan;Cao, Jie;Zhang, Houshuang;Zhou, Jinlin;Wang, Yujian;Li, Xiangrui;Zhou, Jinlin

作者机构：

关键词： Rhipicephalus haemaphysaloides;Cystatin;Inhibitory activity;Embryonic development

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2015 年 8 卷

页码：

收录情况： SCI

摘要： Background: Ticks and tick-borne diseases affect animal and human health worldwide and cause significant economic losses in the animal industry. Functional molecular research is important to understand the biological characteristics of ticks at the molecular level. Enzymes and enzyme inhibitory molecules play very important roles in tick physiology, and the cystatins are tight-binding inhibitors of papain-like cysteine proteases. To this end, a novel cystatin, designated RHcyst-1, was isolated from the tick Rhipicephalus haemaphysaloides. Methods: The full-length gene of RHcyst-1 was cloning by RACE. The recombinant protein of RHcyst-1 was expressed in a glutathione S-transferase (GST)-fused soluble form in Escherichia coli, and its inhibitory activity against cathepsin L, B, C, H, and S, as well as papain, was identified by fluorogenic substrate analysis. Expression analysis of RHcyst-1 at different tick stages was performed by quantitative reverse transcription -PCR (qRT-PCR). An RNAi experiment for RHcyst-1 was performed to determine its function for tick physiology. Results: The full-length cDNA of RHcyst-1 is 471 bp, including an intact open reading frame encoding an expected protein of 98 amino acids, without a signal peptide, having a predicted molecular weight of similar to 11 kDa and an isoelectric point of 5.66. A sequence analysis showed that it has significant homology with the known type 1 cystatins. The results of proteinase inhibition assays showed that rRHcyst-1 can effectively inhibit the six cysteine proteases' enzyme activities. An investigation of the RHcyst-1 genes' expression profile showed that it was more richly transcribed in the embryo (egg) stage. A disruption of the RHcyst-1 gene showed a significant decrease in the rate of tick hatching. Conclusions: Our results suggested that RHcyst-1 may be involved in the early embryonic development of ticks.

分类号：

  • 相关文献

[1]Characterization of a secreted cystatin from the tick Rhipicephalus haemaphysaloides. Wang, Yujian,Yu, Xinmao,Cao, Jie,Zhou, Yongzhi,Gong, Haiyan,Zhang, Houshuang,Zhou, Jinlin,Wang, Yujian,Li, Xiangrui.

[2]Molecular and functional characterization of a cystatin analogue in large yellow croaker (Pseudosciaena crocea). Li, Shuying,Ao, Jingqun,Chen, Xinhua,Li, Shuying,Li, Shuying. 2009

[3]Synthesis and Insecticidal Activity of Novel Carbamate Derivatives as Potential Dual-Binding Site Acetylcholinesterase Inhibitors. Ma, Hong-Ju,Xie, Ru-Liang,Zhao, Qian-Fei,Mei, Xiang-Dong,Ning, Jun,Ma, Hong-Ju.

[4]A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity. Zhou, Jinlin,Ueda, Mami,Umemiya, Rika,Battsetseg, Badgar,Boldbaatar, Damdinsuren,Xuan, Xuenan,Fujisaki, Kozo.

[5]Cloning and prokaryotic expression of Chinese sturgeons cystatin cDNA. Bai, J.,Ma, D.,Lao, H.,Jian, Q.,Ye, X.,Luo, J.. 2005

[6]Identification of Ramie Genes in Response to Pratylenchus coffeae Infection Challenge by Digital Gene Expression Analysis. Yu, Yongting,Zeng, Liangbin,Yan, Zhun,Liu, Touming,Sun, Kai,Zhu, Taotao,Zhu, Aiguo. 2015

[7]Cholinesterase and Human Lung Cancer Cells (A-549) Inhibitory Activity of the Cassava Peel of Euphorbiaceae in Vitro. Chen, Xiao-Ming,Zhang, Zhi-Jun,Li, Shu-Fang,Liu, Ai-Lin,Lv, Fei-Jie. 2016

[8]Design of novel carbamate acetylcholinesterase inhibitors based on the multiple binding sites of acetylcholinesterase. Mei, Xiangdong,Yuan, Huizhu,Ning, Jun,Zhao, Qianfei.

[9]Characterization of Hlcyst-3 as a member of cystatins from the tick Haemaphysalis longicornis. Zhou, Jinlin,Liao, Min,Fujisaki, Kozo,Zhou, Jinlin,Gong, Haiyan,Xuan, Xuenan,Fujisaki, Kozo. 2010

[10]Search for Nodulation and Nodule Development-Related Cystatin Genes in the Genome of Soybean (Glycine max). Yuan, Songli,Li, Rong,Chen, Haifeng,Zhang, Chanjuan,Chen, Limiao,Hao, Qingnan,Shan, Zhihui,Zhang, Xiaojuan,Chen, Shuilian,Yang, Zhonglu,Qiu, Dezhen,Zhou, Xinan,Yuan, Songli,Li, Rong,Chen, Haifeng,Zhang, Chanjuan,Chen, Limiao,Hao, Qingnan,Shan, Zhihui,Zhang, Xiaojuan,Chen, Shuilian,Yang, Zhonglu,Qiu, Dezhen,Zhou, Xinan,Wang, Lei. 2016

[11]Molecular characterization, expression and function analysis of a five-domain Kazal-type serine proteinase inhibitor from pearl oyster Pinctada fucata. Zhang, Dianchang,Ma, Jianjun,Jiang, Shigui. 2014

[12]Cloning and sequencing of a lectin protein gene from the roots of Sophora flavescens. Ma, ZG,Yan, B,Huang, XQ,Wang, LX,Zeng, ZK. 2001

[13]Identification of a cysteine-rich antimicrobial peptide from salivary glands of the tick Rhipicephalus haemaphysaloides. Zhang, Houshuang,Zhang, Wenjie,Wang, Xinzhi,Zhou, Yongzhi,Wang, Na,Zhou, Jinlin. 2011

[14]Proteomic Analysis of Proteins in the Salivary Glands of the Fed and Unfed Female Tick Rhipicephalus haemaphysaloides. Xiang Fei-yu,Zhang Jian-wu,Zhou Yong-zhi,Li Zhuang,Gong Hai-yan,Zhou Jin-lin,Xiang Fei-yu. 2009

[15]Characterization of the anticoagulant protein Rhipilin-1 from the Rhipicephalus haemaphysaloides tick. Gao, Xiao,Shi, Lei,Zhou, Yongzhi,Cao, Jie,Zhang, Houshuang,Zhou, Jinlin.

[16]Identification of Differentially Expressed Genes in the Salivary Gand of Rhipicephalus haemaphysaloides by the Suppression Subtractive Hybridization Approach. Xiang Fei-yu,Zhou Yong-zhi,Zhou Jin-lin. 2012

[17]Differential sialotranscriptomes of unfed and fed Rhipicephalus haemaphysaloides, with particular regard to differentially expressed genes of cysteine proteases. Yu, Xinmao,Gong, Haiyan,Zhou, Yongzhi,Zhang, Houshuang,Cao, Jie,Zhou, Jinlin,Zhou, Jinlin. 2015

[18]Isolation and characterization of two novel serpins from the tick Rhipicephalus haemaphysaloides. Yu, Yingfang,Cao, Jie,Zhou, Yongzhi,Zhang, Honshuang,Zhou, Jinlin. 2013

[19]RNA interference and the vaccine effect of a subolesin homolog from the tick Rhipicephalus haemaphysaloides. Lu, Pengyun,Zhou, Yongzhi,Yu, Yingfang,Cao, Jie,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Lu, Pengyun,Li, Guoqing,Zhou, Jinlin.

[20]BmARM-Like Protein from Silkworm, Bombyx mori (Lepidoptera) is Putatively Involved in Response against BmNPV Infection. Wang, Xue-yang,Zhang, Shang-zhi,Yu, Dong,Ma, Yan,Fei, Dong-qiong,Yu, Hai-zhong,Xu, Jia-ping,Liu, Ming-hui. 2017

作者其他论文 更多>>

微信小程序