PRIMARY ANALYSIS OF THE EXPRESSED SEQUENCE TAGS FROM A FULL-LENGTH ENRICHED cDNA LIBRARY OF SIBERIAN TIGER (PANTHERA TIGRIS ALTAICA)

文献类型： 外文期刊

第一作者： Guo, Y.

作者： Guo, Y.;Liu, C. Q.;Xu, L. S.;Lu, T. F.;Li, X. C.;Ma, Y. H.;Guan, W. J.;Liu, D.;Ma, J. Z.

作者机构：

关键词： Siberian tiger;Fibroblast cell;SMART cDNA library;Expressed sequence tags

期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES （ 影响因子：0.49； 五年影响因子：0.686 ）

ISSN： 1018-7081

年卷期： 2015 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Tiger is a special species only found in Asia and considered as one of the world's most endangered species. In this study, a full-length enriched cDNA library was successfully constructed from Siberian tiger. The titers of primary and amplified libraries were 1.28x10(6) pfu/ml and 1.59x10(10) pfu/ml respectively. The proportion of recombinants from unamplified library was 91.3% and average length of exogenous inserts was 1.06 kb. A total of 365 individual ESTs with sizes ranging from 264 to 1,378bps were then analyzed. Furthermore, 305 unigenes were successfully annotated and involved in 47 functions of the GO classification, Cell (278, 91.1%), Cellular process (302, 82.6%) and Binding (250, 82.0%) are the dominant terms. A total of 234 unigenes were assigned to 244 KEGG pathways, and the pathways with most representation are metabolic pathways (40, 17.1%). Among the 25 COG categories, general function prediction only cluster (52, 14.2%) represents the largest group, followed by translation, ribosomal structure and biogenesis (41, 11.2%), replication, recombination and repair (30, 8.2%), and only 9.9% ESTs (36) classified as novel genes. Moreover, this library provided a useful platform for the functional genome and transcriptome research for the P. tigris and other felid animals in future.

分类号：

  • 相关文献

[1]Generation and analysis of a large-scale expressed sequence tags from a full-length enriched cDNA library of Siberian tiger (Panthera tigris altaica). Guo, Yu,Liu, Changqing,Liu, Changqing,Lu, Taofeng,Bai, Chunyu,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Dan,Bai, Chunyu.

[2]Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger. Liu, Chang-Qing,Lu, Tao-Feng,Feng, Bao-Gang,Guan, Wei-Jun,Ma, Yue-Hui,Liu, Chang-Qing,Liu, Dan. 2010

[3]Construction of a Full-Length Enriched cDNA Library and Preliminary Analysis of Expressed Sequence Tags from Bengal Tiger Panthera tigris tigris. Liu, Changqing,Lu, Taofeng,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Changqing,Guo, Yu,Liu, Dan,Zhang, Minghai,Ma, Jianzhang. 2013

[4]Establishment and Biological Characteristic Research of Yuxizhiwei sheep Fibroblast Cell Bank. Wang, Hui,Li, Xiangchen,Li, Changli,Zhang, Wenxiu,Guan, Weijun,Ma, Yuehui,Wang, Hui. 2012

[5]Molecular characterization of a feline calicivirus isolated from tiger and its pathogenesis in cats. Tian, Jin,Liu, Dafei,Liu, Yongxiang,Wu, Hongxia,Jiang, Yanmei,Zu, Shaopo,Liu, Chunguo,Sun, Xue,Liu, Jiasen,Qu, Liandong,Zu, Shaopo,Sun, Xue,Jiang, Yanmei.

[6]De novo transcriptomic analysis and development of EST-SSR markers in the Siberian tiger (Panthera tigris altaica). Ma, Yuehui,Guan, Weijun,Lu, Taofeng,Ma, Qin,Zhu, Minghao,Chen, Hongyan,Sun, Yujiao,Ma, Jianzhang,Liu, Dan.

[7]Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis. Wang, Hui,Liu, Zheng,Wang, Hui,Zhang, Wenxiu,Yuan, Ziao,Yuan, Hongyi,Liu, Xueting,Guan, Weijun,Yang, Chunwen. 2016

[8]Complete mitochondrial genome of a wild Siberian tiger. Sun, Yujiao,Sun, Zhaohui,Liu, Zhensheng,Teng, Liwei,Lu, Taofeng,Guan, Weijun,Wang, Shuo,Ma, Yuehui. 2015

[9]Sterile nodular panniculitis with lung and lymph node involvement in a Siberian tiger (Panthera tigris altica). Hu, Shou-Ping,Zhang, Zhuo,Zhang, Jiao-Er,Cai, Xue-Hui,He, Xi-Jun,Nakayama, Hiroyuki. 2017

[10]Lead induces Siberian tiger fibroblast apoptosis by interfering with intracellular homeostasis. Liu, Zheng,Wang, Hui,Guo, Xuesong,Zhang, Wenxiu,Yuan, Ziao,Yuan, Hongyi,Liu, Xueting,Guan, Weijun,Zhang, Minghai. 2018

[11]Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage. Liu, Changqing,Bai, Chunyu,Lu, Taofeng,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Changqing,Guo, Yu,Liu, Dan,Ma, Jianzhang. 2014

[12]Identification of Expressed Resistance Gene Analogs from Peanut (Arachis hypogaea L.) Expressed Sequence Tags. Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Chen, Xiaoping,Culbreath, Albert K.,Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Varshney, Rajeev K.,Chen, Xiaoping,Guo, Baozhu. 2013

[13]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li#2;,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[14]Immune gene discovery by expressed sequence tag (EST) analysis of hemocytes in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei. 2013

[15]DEVELOPMENT AND CHARACTERIZATION OF 41 NOVEL EST-SSR MARKERS FOR PISUM SATIVUM (LEGUMINOSAE). Xu, Sheng-Chun,Gong, Ya-Ming,Hu, Qi-Zan,Zhang, Gu-Wen,Mao, Wei-Hua,Fu, Wan,Xian, Qiang-Qiang,Fu, Wan,Xian, Qiang-Qiang. 2012

[16]Identification of differentially expressed genes in Mongolian sheep ovaries by suppression subtractive hybridization. He, Xiaolong,Wang, Feng,Tian, Chunying,Rong, Weiheng,Liu, Yongbin,He, Xiaolong,Li, Bei. 2012

[17]Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils. Jiang, YQ,Ma, RC. 2003

[18]SSR data mined from expressed sequence tags of phytoparasitic nematodes. Huang, Q. X.,Cheng, X. Y.,Xu, R. M.,Xie, B. Y.. 2010

[19]Erwinia carotovora ssp. Carotovora infection induced "defense lignin" accumulation and lignin biosynthetic gene expression in chinese cabbage (Brassica rapa L. ssp pekinensis). Zhang, Song-He,Yang, Qing,Ma, Rong-Cai. 2007

[20]Progress in expressed sequence tags (EST) project of plant genome. Luo, M,Jia, JZ. 2001

作者其他论文 更多>>

微信小程序