Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa

文献类型： 外文期刊

第一作者： Huang, B. L.

作者： Huang, B. L.;Cheng, C.;Zhang, G. Y.;Su, J. J.;Zhi, Y.;Xu, S. S.;Cai, D. T.;Huang, B. Q.;Zhang, X. K.

作者机构：

关键词： Eruca vesicaria subsp sativa;Nicotianamine synthase;Gene cloning

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. In this study, a gene encoding EvNAS was cloned and characterized in E. vesicaria subsp sativa. The full-length EvNAS cDNA sequence contained a 111-bp 5'-untranslated region (UTR), a 155-bp 3'-UTR, and a 966-bp open reading frame encoding 322-amino acid residues. The EvNAS genomic sequence contained no introns, which is similar to previously reported NAS genes. The deduced translation of EvNAS contained a well-conserved NAS domain (1-279 amino acids) and an LIKI-CGEAEG box identical to some Brassica NAS and to the LIRL-box in most plant NAS, which is essential for DNA binding. Phylogenetic analysis indicated that EvNAS was most closely related to Brassica rapa NAS3 within the Cruciferae, followed by Thlaspi NAS1, Camelina NAS3, and Arabidopsis NAS3. A reverse transcription-polymerase chain reaction indicated that EvNAS expression was greatest in the leaves, followed by the flower buds and hypocotyls. EvNAS was moderately expressed in the roots.

分类号：

  • 相关文献

[1]Cloning and characterization of the dehydration-responsive element-binding protein 2A gene in Eruca vesicaria subsp sativa. Huang, B. L.,Li, Y. Y.,Li, D. Y.,Ma, M. Y.,Cai, D. T.,Wu, W. H.,Huang, B. Q.,Zhang, X. K.. 2016

[2]Genome-wide identification, classification and expression profiling of nicotianamine synthase (NAS) gene family in maize. Zhou, Xiaojin,Zhao, Qianqian,Liu, Xiaoqing,Zhang, Shaojun,Sun, Cheng,Fan, Yunliu,Zhang, Chunyi,Chen, Rumei,Zhou, Xiaojin,Zhao, Qianqian,Liu, Xiaoqing,Zhang, Shaojun,Sun, Cheng,Fan, Yunliu,Zhang, Chunyi,Chen, Rumei,Li, Suzhen. 2013

[3]Nicotianamine synthase gene family as central components in heavy metal and phytohormone response in maize. Zhou, Mei-Liang,Qi, Lei-Peng,Zhang, Qian,Shao, Ji-Rong,Zhou, Mei-Liang,Pang, Jun-Feng,Lei, Zhi,Tang, Yi-Xiong,Wu, Yan-Min,Zhou, Mei-Liang,Zhu, Xue-Mei.

[4]Cloning and sequence analysis of putative type II fatty acid synthase genes from Arachis hypogaea L.. Li, Meng-Jun,Li, Ai-Qin,Xia, Han,Zhao, Chuan-Zhi,Li, Chang-Sheng,Wan, Shu-Bo,Bi, Yu-Ping,Wang, Xing-Jun. 2009

[5]Identification, cloning and characterization of a novel Gossypium hirsutum L. GhMYBantiV transcription factor in response to biotic and abiotic stresses. Li Fei,Chen Xiaoyan,He Xiaohong,Tang Ming,Hong Kun,Yang Zhengting,Yi Yin,Liu Zhanji,Fu Mingchuan. 2017

[6]Molecular Cloning and Characterization of a New Cold-active Extradiol Dioxygenase from a Metagenomic Library Derived from Polychlorinated Biphenyl-contaminated Soil. Ren He-jun,Zhou Rui,Wang Yan,Zhang Lan-ying,Lu Yang,Dai Chun-yan. 2012

[7]Characterization and cloning of SMALL GRAIN 4, a novel DWARF11 allele that affects brassinosteroid biosynthesis in rice. Shi, Zhenyuan,Rao, Yuchun,Xu, Jie,Hu, Shikai,Fang, Yunxia,Yu, Haiping,Pan, Jiangjie,Liu, Ruifang,Ren, Deyong,Wang, Xiaohu,Zhu, Yangzhou,Zhu, Li,Dong, Guojun,Zhang, Guangheng,Zeng, Dali,Guo, Longbiao,Hu, Jiang,Qian, Qian,Rao, Yuchun,Zhu, Yangzhou,Xu, Jie. 2015

[8]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

[9]Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). Jin, YM,Lin, JJ,Feng, XG,Zhang, L,Wu, XF,Zhou, YC,Cai, YM. 2002

[10]The Research on Actinidia eriantha Benth. in South China. Wu, Y. J.,Xie, M.,Zhang, Q. C.,Zhang, H. Q.,Fang, J. B.. 2011

[11]VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Liu, Guanshan,Chen, Jia,Wang, Xuechen. 2006

[12]Cloning and expression of actin gene of Schistosoma japonicum Chinese strain. Zhu, JG,Lin, JJ,Feng, XG,Wu, XF,Zhou, YC,Cai, YM. 2000

[13]Lipopolysaccharide and beta-1,3-glucan binding protein in the hard clam (Meretrix meretrix): Molecular characterization and expression analysis. Liu, S. X.,Zhang, J. J.,Li, H. J.,Qi, Z. H.,He, C. B.,Gao, X. G.. 2014

[14]Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun. 2014

[15]MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice. Yu, Haiping,Zhu, Yangzhou,Xu, Jiangmin,Lu, Mei,Ma, Bojun,Rao, Yuchun,Yu, Haiping,Ren, Deyong,Zhu, Yangzhou,Wang, Yuexing,Liu, Ruifang,Fang, Yunxia,Shi, Zhenyuan,Pan, Jiangjie,Hu, Jiang,Rao, Yuchun,Yu, Haiping,Yu, Haiping. 2016

[16]A novel functional gene associated with cold tolerance at the seedling stage in rice. Zhao, Junliang,Zhang, Shaohong,Dong, Jingfang,Yang, Tifeng,Mao, Xingxue,Liu, Qing,Wang, Xiaofei,Liu, Bin,Zhao, Junliang,Zhang, Shaohong,Dong, Jingfang,Yang, Tifeng,Mao, Xingxue,Liu, Qing,Wang, Xiaofei,Liu, Bin. 2017

[17]Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein. Fang, Ji-Chao,Cai, Ping-Zhong,Yan, Wen-Zhao,Wu, Jie,Guo, Hui-Fang. 2005

[18]The chitinase C gene PsChiC from Pseudomonas sp and its synergistic effects on larvicidal activity. Zhong, Wanfang,Guo, Huifang,Zhong, Wanfang,Ding, Shaojun. 2015

[19]Isolation of anthocyanin biosynthetic genes in red Chinese sand pear (Pyrus pyrifolia Nakai) and their expression as affected by organ/tissue, cultivar, bagging and fruit side. Yu, Bo,Zhang, Dong,Qian, Minjie,Zheng, Xiaoyan,Teng, Yuanwen,Huang, Chunhui,Su, Jun,Shu, Qun. 2012

[20]Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus). Qiu, LH,Song, LS,Wu, LT,Cai, ZH,Jiang, SG. 2006

作者其他论文 更多>>

微信小程序