Cross Protection Experiment in Mice Immunization with Actinobacillus pleuropneumoniae serotype 7 Genomic Expression Library

文献类型： 外文期刊

第一作者： Liu, Huifang

作者： Liu, Huifang;Liu, Huifang;Si, Wei;Zhou, Yuanyuan;Wang, Chunlai;Liu, Siguo

作者机构：

关键词： Actinobacillus pleuropneumoniae;genomic expression library;antibodies and IFN-gamma;viable bacteria;survival rate

期刊名称：INTERNATIONAL JOURNAL OF APPLIED RESEARCH IN VETERINARY MEDICINE （ 影响因子：0.306； 五年影响因子：0.339 ）

ISSN： 1542-2666

年卷期： 2015 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Actinobacillus (A.) pleuropneumoniae is a causative agent of porcine pleuropneumonia, which has been reported in most countries. A genomic expression library of A. pleuropneumonia serotype 7 was constructed to identify potential vaccine candidates. Plasmid DNA from the sub-libraries (L1 to L10) was used to immunize BALB/c mice. Then, 2 weeks after the final immunization, the groups of mice were challenged with A. pleuropneumonia serotype 1 (1x109 cfu). Mice immunized with one of the sub-libraries (L3) had a higher level of antibodies and IFN-gamma production than others. This sub-library (L3) resulted in a significant reduction in the amount of A. pleuropneumoniae recovered from mouse lungs and the survival rate of the L3 group was the most highest. Our study demonstrates that a genome expression library of A. pleuropneumoniae serotype 7 offers a novel approach for screening possible cross-serotype protection vaccine candidates against A. pleuropneumoniae serotype 1 infection.

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