Production of early monozygotic twin bovine embryos in vitro by the blastomere separation and coculture technique

文献类型： 外文期刊

第一作者： Zhao Shan-jiang

作者： Zhao Shan-jiang;Zhao Xue-ming;Du Wei-hua;Hao Hai-sheng;Liu Yan;Qin Tong;Wang Dong;Zhu Hua-bin

作者机构：

关键词： cattle;in vitro embryo culture;blastomere separation;microwell;coculture

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 10 期

页码：

收录情况： SCI

摘要： The objective of this study was to establish an efficient system of producing early monozygotic twin bovine embryos in vitro using the blastomere separation and coculture technique. In this study, early eight-cell embryos were chosen to optimize the separation method, and multi-coculture tactics were applied to improve the efficiency of this production system. Bovine embryo blastomeres (groups of at least 30 at the eight-cell stage) were separated into eight segments (to regard an eight-cell embryo as a tangerine, a blastomere as one segment) and one, two and four segments (blastomeres) were cultured respectively in microwells on the bottom of the four-well dish (Nunc, Denmark) with 400 mu L of culture medium under paraffin oil. Four different types of coculture tactics (cocultured with nothing, intact embryos, bovine cumulus cells (bCCs), intact embryos & bCCs) were applied to the group of four segments (blastomeres). Finally, diameter and inner cell mass (ICM):trophectoderm (TE) cell ratio was measured as a criterion to assess the quality of the twin embryos which were derived from bovine separated blastomeres. Our results showed that rate of blastocyst formation of the four segments group was significantly greater than one or two group (P<0.05). In addition, rate of blastocyst formation was significantly increased when the four segments were cocultured with intact embryo & bCCs (P<0.05). Although the ICM, TE and total cells of blastocysts derived from separated blastomeres was less than the control group from intact embryo (P<0.05), more important quality indicator of the blastocyst diameter and ICM:TE cell ratio was similar between our experimental group and the control group (P>0.05). Thus, these results suggest that combined with intact embryos & bCCs coculture system, culturing four isolated segments (blastomeres) per microwell is an efficient system of producing early monozygotic twin bovine embryos. Furthermore, our results also indicate that the quality of blastocysts derived from separated blastomere may be similar to those derived from intact eight-cell embryos.

分类号：

  • 相关文献

[1]A SNP in intron 8 of CD46 causes a novel transcript associated with mastitis in Holsteins. Wang, Xiuge,Zhong, Jifeng,Gao, Yundong,Ju, Zhihua,Huang, Jinming,Huang, Jinming. 2014

[2]Variants and Gene Expression of the TLR2 Gene and Susceptibility to Mastitis in Cattle. Huang, Jinming,Liu, Li,Wang, Hongmei,Zhang, Cuixia,Ju, Zhihua,Wang, Changfa,Zhong, Jifeng.

[3]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[4]Identification of differentially expressed proteins between bull X and Y spermatozoa. Chen, Xiaoli,Zhu, Huabin,Hao, Haisheng,Zhao, Xueming,Du, Weihua,Qin, Tong,Liu, Yan,Wang, Dong,Chen, Xiaoli,Wu, Chengjiang,Han, Weidong. 2012

[5]Protecting Local Breeds of Livestock in NW China. Lang Xia,Wang Cailian,Squires, Victor. 2010

[6]Genome-wide detection of copy number variations using high-density SNP genotyping platforms in Holsteins. Jiang, Li,Jiang, Jicai,Yang, Jie,Liu, Xuan,Wang, Haifei,Ding, Xiangdong,Liu, Jianfeng,Zhang, Qin,Wang, Jiying. 2013

[7]Effects of N-carbamylglutamate and L-arginine on steroidogenesis and gene expression in bovine granulosa cells. Feng, T.,Schutz, L. F.,Morrell, B. C.,Perego, M. C.,Spicer, L. J.. 2018

[8]Molecular cloning, expression and characterization of bovine UQCC and its association with body measurement traits. Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Liu, Yongfeng,Huang, Honggang,Li, Yong,Tang, Zhonglin,Yang, Shulin,Li, Kui,Zan, Linsen. 2010

[9]Comparative Mapping and 3 ' UTR SNP Detection of ANGPTL4 Gene in Beef Cattle. Ma, Y.,Li, R. R.,Hou, F.,Zhang, Y. H.,Chen, H.,Ma, Y.,Xu, S. Z.,Cheng, N.. 2011

[10]Genetic Polymorphisms of the CACNA2D1 Gene and Their Association with Carcass and Meat Quality Traits in Cattle. Hou, Guan-Yu,Yuan, Zheng-Rong,Gao, Xue,Li, Jun-Ya,Gao, Hui-Jiang,Chen, Jin-Bao,Xu, Shang-Zhong,Hou, Guan-Yu. 2010

[11]An isolation, polymorphism and trait association study of bovine GAD1 and GAL genes. Guo, H.,Li, K.,Liu, W-S.,Li, H-D.,Xu, S-Z.,Ren, H-Y.,Gao, X.. 2006

[12]Characterization and mapping of the bovine FBP1 gene. Guo, H.,Liu, W-S.,Takasuga, A.,Eyer, K.,Landrito, E.,Xu, S-Z.,Gao, X.,Ren, H-Y.. 2007

[13]cDNA Microarray on Differentially Expressed Genes of Liver Tissue Between Bulls and Steers of Simmental. Wang, Shuhui,Lian, Zhengxing,Wang, Shuhui,Zhou, Zhengkui,Huai, Yahong,Ji, Aiguo,Gao, Xue,Li, Junya,Chen, Jinbao,Xu, Shangzhong,Wang, Shuxin. 2011

[14]The effect of cysteamine hydrochloride and nitrate supplementation on in-vitro and in-vivo methane production and productivity of cattle. Sun, Y. K.,Yan, X. G.,Ban, Z. B.,Yang, H. M.,Zhao, Y. M.,Sun, Y. K.,Hegarty, R. S.. 2017

[15]Associations of GBP2 gene copy number variations with growth traits and transcriptional expression in Chinese cattle. Zhang, Gui-Min,He, Hua,Song, Cheng-Chuang,Cao, Xiu-Kai,Lei, Chu-Zhao,Lan, Xian-Yong,Chen, Hong,Huang, Yong-Zhen,Zheng, Li,He, Hua,Zhang, Zi-Jing,Qi, Xing-Lei. 2018

[16]Investigation on BRCA1 SNPs and its effects on mastitis in Chinese commercial cattle. Yuan, Zhengrong,Li, Junya,Li, Jiao,Zhang, Lupei,Gao, Xue,Gao, Hui Jiang,Xu, Shangzhong,Yuan, Zhengrong.

[17]Application of a NMR-based untargeted quantitative metabonomic approach to screen for illicit salbutamol administration in cattle. Tang, Chaohua,Zhang, Kai,Liang, Xiaowei,Zhao, Qingyu,Zhang, Junmin.

[18]SNPs identification and its correlation analysis with milk somatic cell score in bovine MBL1 gene. Yuan, Zhengrong,Li, Jiao,Li, Junya,Gao, Xue,Xu, Shangzhong,Yuan, Zhengrong,Yuan, Zhengrong.

[19]Evaluating the Babesia bovis infection of cattle in China with enzyme-linked immunosorbent assay (ELISA). Yang, Congshan,Liu, Junlong,Li, Anyan,Li, Youquan,Liu, Aihong,Xie, Junren,Liu, Guangyuan,Yin, Hong,Guan, Guiquan,Luo, Jianxun,Yin, Hong,Luo, Jianxun.

[20]Copy number variations at LEPR gene locus associated with gene expression and phenotypic traits in Chinese cattle. Shi, Tao,Xu, Yao,Yang, Mingjuan,Huang, Yongzhen,Lan, Xianyong,Lei, Chuzhao,Chen, Hong,Yang, Xiaoming,Qi, Xinglei.

作者其他论文 更多>>

微信小程序