Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library

文献类型： 外文期刊

第一作者： Gao, Y. H.

作者： Gao, Y. H.;Wang, K. F.;Zhang, S.;Fan, Y. N.;Guan, W. J.;Ma, Y. H.;Gao, Y. H.;Wang, K. F.;Zhang, S.;Fan, Y. N.

作者机构：

关键词： cDNA library;Hepatocytes;Liver fatty acid binding proteins;Wuzhishan miniature pig

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 3 期

页码：

收录情况： SCI

摘要： Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expression vector (pEGFP-N3-L-FABP) was constructed in vitro. This vector was transfected into hepatocytes to test its function. The results of western blotting analysis demonstrated that the L-FABP gene from our full-length enriched cDNA library regulated downstream genes, including the peroxisome proliferator-activated receptor family in hepatocytes. This study provides a theoretical basis and experimental evidence for the application of L-FABP for the treatment of liver injury.

分类号：

  • 相关文献

[1]Hepatoprotective and antioxidant effects of Glycyrrhiza glabra extract against carbon tetrachloride (CCl4)-induced hepatocyte damage in common carp (Cyprinus carpio). Yin, Guojun,Lu, Chengping,Yin, Guojun,Cao, Liping,Xu, Pao,Jeney, Galina,Nakao, Miki. 2011

[2]Leptin stimulates hepatic activation of thyroid hormones and promotes early posthatch growth in the chicken. Li, Rongjie,Hu, Yan,Ni, Yingdong,Zhao, Ruqian,Li, Rongjie,Xia, Dong,Hu, Yan,Grossmann, Roland.

[3]Genetic characteristics of inbred Wuzhishan miniature pigs, a native Chinese breed. Wang, Xilong,Ou, Jiangtao,Huang, Liguang,Nishihara, Masugi,Li, Junyou,Manabe, Noboru,Zhang, Yuan. 2006

[4]Isolation and biological characterization of a novel type of pulmonary mesenchymal stem cells derived from Wuzhishan miniature pig embryo. Ma, Caiyun,Liu, Hao,Yang, Jinjuan,Li, Xiangchen,Liu, Changqing,Guan, Weijun,Guo, Yu,Liu, Changqing,Guo, Yu,Wang, Kunfu.

[5]Establishment and genetic characteristics analysis of in vitro culture a fibroblast cell line derived from Wuzhishan miniature pig. Liu, Changqing,Guo, Yu,Liu, Changqing,Lu, Taofeng,Li, Xiangchen,Guan, Weijun,Ma, Yuihui.

[6]Purification, characterisation and protective effects of polysaccharides from alfalfa on hepatocytes. Wang, Shaopu,Dong, Xiaofang,Cui, Yaoming,Tong, Jianming,Ma, Hao.

[7]Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes. Jin, Xiao-Lu,Wang, Kai,Li, Qiang-Qiang,Tian, Wen-Li,Xue, Xiao-Feng,Wu, Li-Ming,Jin, Xiao-Lu,Hu, Fu-Liang.

[8]Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene. Zhang, Yue,Wang, Xingjun,Zhang, Yue,Xia, Han,Zhao, Chuanzhi,Li, Aiqin,Wang, Xingjun,Yuan, Mei. 2012

[9]Isolation and expression analysis of LEA genes in peanut (Arachis hypogaea L.). Su, Lei,Bi, Yu-Pin,Wang, Xing-Jun,Su, Lei,Zhao, Chuan-Zhi,Bi, Yu-Pin,Wan, Shu-Bo,Xia, Han,Wang, Xing-Jun,Su, Lei,Zhao, Chuan-Zhi,Bi, Yu-Pin,Wan, Shu-Bo,Xia, Han,Wang, Xing-Jun,Su, Lei,Zhao, Chuan-Zhi,Bi, Yu-Pin,Wan, Shu-Bo,Xia, Han,Wang, Xing-Jun.

[10]Construction of early human embryo cDNA libraries and screening objective genes. Gou, DM,Huang, J,Li, WX,Mao, X,Jiang, DH,Hua, WJ,Fan, JH. 1999

[11]Isolation of cDNA sequences encoding the MAP kinase HOG1 and the MAP kinase kinase PBS2 genes of the fungus Alternaria tenuissma through a genetic approach. Qiu, Dewen,Jiang, Linghuo. 2007

[12]Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library. Tao, Bo,Shao, Bai-Hui,Qiao, Yu-Xin,Wang, Xiao-Qin,Chang, Shu-Jun,Qiu, Li-Juan. 2017

[13]Construction of cDNA Library and Analysis of SSRs in ESTs of Litchi (Litchi chinensis Sonn.). Xiang, X.,Sun, Q. M.,Ou, L. X.,Zhao, J. S.,Chen, J. Z.,Cai, C. H.. 2012

[14]Constructing and random sequencing analysis of normalized cDNA library of testis tissue from oriental river prawn (Macrobrachium nipponense). Qiao, Hui,Fu, Hongtuo,Jin, Shubo,Wu, Yan,Jiang, Sufei,Gong, Yongsheng,Xiong, Yiwei.

[15]Small-scale Expressed Sequence Tag Analysis Of Theileria Uilenbergi: Identification Of A Gene Family Encoding Potential Antigenic Proteins. Liu, Zhijie,Ahmed, Jabbar S.,Seitzer, Ulrike,Liu, Zhijie,Dang, Zhisheng,Luo, Jianxian,Yin, Hong.

[16]Identification and function analysis of the host cell protein that interacted with Orf virus Bcl-2-like protein ORFV125. Tian, Hong,Chen, Yan,Wu, Jinyan,Lin, Tong,Liu, Xiangtao.

[17]Identification and expression pattern analysis of chemosensory receptor genes in the Macrocentrus cingulum (Hymenoptera: Braconidae) antennae. Zhang, Tian-tao,Wang, Zhen-ying,He, Kang-lai,Bai, Shu-xiong,Ahmed, Tofael. 2016

[18]Identification of Differentially Expressed Genes in the Salivary Gand of Rhipicephalus haemaphysaloides by the Suppression Subtractive Hybridization Approach. Xiang Fei-yu,Zhou Yong-zhi,Zhou Jin-lin. 2012

[19]Generation and analysis of expressed sequence tags from the tender shoots cDNA library of tea plant (Camellia sinensis). Chen, L,Zhao, LP,Gao, QK.

[20]Identification of the heat shock protein 70 (HLHsp70) in Haemaphysalis longicornis. Tian, Zhancheng,Liu, Guangyuan,Zhang, Liyan,Yin, Hong,Xie, Junren,Zhang, Ping,Luo, Jin,Wang, Hui.

作者其他论文 更多>>

微信小程序