Molecular characterization of two candidate genes associated with coat color in Tibetan sheep (Ovis arise)

文献类型： 外文期刊

第一作者： Han Ji-long

作者： Han Ji-long;Guo Ting-ting;Yue Yao-jing;Liu Jian-bin;Niu Chun-e;Yang Bo-hui;Yang Min;Wang Chao-feng

作者机构：

关键词： Tibetan sheep;KIT;MITF;gene polymorphism;gene expression;coat color

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Coat color is a key economic trait in sheep. Some candidate genes associated with animal's coat color were found. Particularly, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene honnolog (KIT) and microphthalmia-associated transcription factor (MITF) play a key role in the modulation of hair pigmentation in mammals. This study investigated those two candidate genes' mutations and expressions associated with wool color in Tibetan sheep. First, the gene polymorphisms of those two genes were analyzed, and then, relative mRNA expression levels of those two genes in skin tissue with different coat colors were compared. Thirdly, KIT and MITF protein expression levels were detected through Western blot and immune-histochennical. Allele C was predominant allele in the white coat color Tibetan sheep population of the MITF coding region g. 1548 C/T loci. The relative MITFnnRNA expression in black coat skin tissue was significantly higher than white (P<0.01). However, no significant differences were detected in the KIT gene's mRNA expression of these two different coat color skin tissues (P>0.05), while the level of KIT protein expression in skin tissues of white and black coats was also roughly equivalent. Our study observed that, the level of MITF protein expression in black coat skin tissue was significantly higher than that in white coat skin tissue, and positive staining for MITF protein expression was detected mainly in the epidermis and the dermal papilla, bulb, and outer root sheath of hair follicles. We conclude that the black coat of Tibetan sheep is related to high MITF expression in the hair follicles, and MITF may be important for coat color formation of Tibetan sheep.

分类号：

  • 相关文献

[1]Tyrosinase-related protein 1 (TYRP1) gene polymorphism and skin differential expression related to coat color in Mongolian horse. Li, Bei,Zhao, Yi-ping,Zhao, Qi-nan,Unierhu,Bai, Dong-yi,Manglai, Dugarjaviin,He, Xiao-long.

[2]Phenotypic and Genotypic Characterization of Phytophthora infestans Isolates from China. Li, Benjin,Chen, Qinghe,Lv, Xin,Lan, Chengzhong,Zhao, Jian,Qiu, Rongzhou,Weng, Qiyong.

[3]Regulatory pathway analysis of coat color genes in Mongolian horses. Li, Bei,Zhao, Yiping,Bai, Dongyi,Shiraigo, Wunierfu,Zhao, Qinan,Manglai, Dugarjaviin,He, Xiaolong. 2017

[4]Genetic diversity within Oryza rufipogon germplasms preserved in Chinese field gene banks of wild rice as revealed by microsatellite markers. Zhang, Chi-Hong,Li, Dao-Yuan,Pan, Da-Jian,Jia, Ji-Zeng,Dong, Yu-Shen.

[5]Analysis of agouti signaling protein (ASIP) gene polymorphisms and association with coat color in Tibetan sheep (Ovis aries). Han, J. L.,Yue, Y. J.,Guo, T. T.,Liu, J. B.,Niu, C. E.,Yang, B. H.,Yang, M.. 2015

[6]Compare of Phenotypic Variation with the Polymorphism of One Coagulation Related Gene Locus in Buffalo Kappa-Casein. Lin, Bo,Yang, Bingzhuang,Li, Ling,Yan, Tang,Long, Haoru,Ren, Daxi,Zeng, Qingkun. 2013

[7]Polymorphism in exon 2 of INHBB gene and its relationship with litter size in Jining Grey goats. Chu, M. X.,Fang, L.,Di, R.,Cao, G. L.,Feng, T.,Peng, Z. L.,Chen, H. Q.,Zhang, Y. J.,Li, N.. 2012

[8]Identification and pharmacological analyses of eight naturally occurring caprine melanocortin-1 receptor mutations in three different goat breeds. Xiong, Qi,Chen, Mingxin,Xiong, Qi,Chai, Jin,Tao, Ya-Xiong,Chai, Jin,Chai, Jin.

[9]First Report of Coxiella burnetii Seroprevalence in Tibetan Sheep in China. Yin, Ming-Yang,Qin, Si-Yuan,Tan, Qi-Dong,Feng, Sheng-Yong,Liu, Guang-Xue,Zhou, Dong-Hui,Zhu, Xing-Quan,Yin, Ming-Yang,Zhu, Xing-Quan,Qin, Si-Yuan,Tan, Qi-Dong. 2015

[10]Hematologic, Serum Biochemical Parameters, Fatty Acid and Amino Acid of Longissimus dorsi Muscles in Meat Quality of Tibetan Sheep. Wang, Hui,Huang, Meizhou,Li, Shengkun,Wang, Shengyi,Dong, Shuwei,Cui, Dongan,Qi, Zhiming,Liu, Yongming. 2015

[11]Reducing methane emissions and the methanogen population in the rumen of Tibetan sheep by dietary supplementation with coconut oil. Long, Ruijun,Huang, Xiaodan,Guo, Xusheng,Mi, Jiandui,Ding, Xuezhi,Zhang, Qian,Ding, Xuezhi,Zhang, Qian.

[12]Detecting signatures of selection within the Tibetan sheep mitochondrial genome. Niu, Lili,Xiao, Ping,Zhou, Jingxuan,Hu, Jiangtao,Guo, Jiazhong,Li, Li,Wang, Linjie,Zhang, Hongping,Zhong, Tao,Chen, Xiaoyong,Sun, Hongxin,Zhao, Qianjun.

[13]Meta-analysis evidence of maternal lineages in Chinese Tibetan sheep using mtDNA D-loop panel. Guangxin, E.,Zhao Yong-Ju,Na Ri-Su,Zhang Jia-Hua,Chen Li-Peng,Qiu Xiao-Yu,Zhao Zhang-Quan,Sun Ya-Wang,Wu Xin,Huang Yong-Fu,Ma Yue-Hui.

[14]Effects of Long-Term Mineral Block Supplementation on Antioxidants, Immunity, and Health of Tibetan Sheep. Wang, Hui,Huang, Meizhou,Wang, Shengyi,Cui, Dongan,Dong, Shuwei,Li, Shengkun,Qi, Zhiming,Liu, Yongming,Liu, Zhiqi,Wang, Hui.

[15]Removal of early fruiting branches impacts leaf senescence and yield by altering the sink/source ratio of field-grown cotton. Chen, Yizhen,Dong, Hezhong,Chen, Yizhen,Kong, Xiangqiang,Dong, Hezhong,Kong, Xiangqiang,Dong, Hezhong. 2018

[16]Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803. Chen, Gao,Bian, Fei,Peng, Zhenying,Zhang, Yan,Yu, Jinhui,Xuan, Ning,Bi, Yuping,He, Qingfang,Chen, Gao,Bian, Fei,Peng, Zhenying,Zhang, Yan,Yu, Jinhui,Xuan, Ning,Bi, Yuping,He, Qingfang,He, Qingfang,Wang, Qiang,Qu, Shujie,Ge, Haitao. 2014

[17]Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803. Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Jun,Qin, Song,Chen, Jun,He, Qingfang. 2017

[18]Transcriptome Analysis of Sucrose Metabolism during Bulb Swelling and Development in Onion (Allium cepa L.). Zhang, Chunsha,Zhang, Hongwei,Liang, Yi,Zhan, Zongxiang,Liu, Bingjiang,Chen, Zhentai. 2016

[19]Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP. Xiaoding Ma,Chaozhu Xing,Liping Guo,Yangcang Gong,Hailin Wang,Yunlei Zhao,Jianyong Wu. 2007

[20]Exogenous nitric oxide delays salt-induced leaf senescence in cotton (Gossypium hirsutum L.). Kong, Xiangqiang,Wang, Tao,Li, Weijiang,Tang, Wei,Zhang, Dongmei,Dong, Hezhong,Wang, Tao,Dong, Hezhong.

作者其他论文 更多>>

微信小程序