Cloning and analysis of the soybean MEKK gene

文献类型： 外文期刊

第一作者： Sha, A. -H.

作者： Sha, A. -H.;Ba, H. -P.;Shan, Z. -H.;Chen, H. -F.;Chen, S. -L.;Qiu, D. -Z.;Zhou, X. -A.;Sha, A. -H.;Chen, Y. -H.

作者机构：

关键词： Mitogen-activated protein kinase;Subcellular localization;Serine/threonine protein kinase;Real-time fluorescent quantitative PCR;Semi-quantitative PCR

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 2 期

页码：

收录情况： SCI

摘要： In this paper, homologous cloning methods were used to clone the soybean GmMEKK gene, which possesses a high degree of similarity to Arabidopsis thaliana AtMEKK1. AtMEKK1 is formed by 595 amino acids, and its secondary structure is formed by 38 irregular curls, 24 alpha helix, 14 beta, with S-TKc domain, transmembrane domain and does not have membrane spanning domain and signal peptide. GmMEKK-GFP subcellular localization fusion and prokaryotic expression vectors were generated and it was revealed that GmMEKK encodes a highly conserved 66.8-kDa nuclear protein that is expressed in soybean roots, stem floral pieces, and leaves. A real-time quantitative PCR analysis of GmMEKK under different abiotic stresses revealed that the expression level of GmMEKK increased under drought and low phosphorus and nitrogen conditions. Taken together, these data suggest that GmMEKK may play an important role in the soybean abiotic stress response.

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