MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1S(L) GENOME OF Aegilops longissima

文献类型： 外文期刊

第一作者： Zhu, Gengrui

作者： Zhu, Gengrui;Wang, Shunli;Zhen, Shoumin;Shen, Xixi;Yan, Yueming;Wang, Shunli;Prodanovic, Slaven;Yan, Yueming

作者机构：

关键词： Ae. longissima;HMW-GS;molecular clone;phylogenetics

期刊名称：GENETIKA-BELGRADE （ 影响因子：0.761； 五年影响因子：0.678 ）

ISSN： 0534-0012

年卷期： 2015 年 47 卷 1 期

页码：

收录情况： SCI

摘要： Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.

分类号：

  • 相关文献

[1]Identification and molecular characterization of one novel 1S(1)-encoded s-type low molecular weight glutenin B-subunit from 1S(1)(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum). Deng, Xiong,Wang, Shun-li,Zhen, Shou-min,Yan, Yue-ming,Zhang, Wen-ying,Yan, Yue-ming,Wang, Shun-li.

[2]Complete nucleotide sequence of a novel porcine circo-virus-like agent and its infectivity in vitro. Wen LiBin,He KongWang,Ni YanXiu,Zhang XueHan,Guo RongLi,Pan QunXin,Yang HanChun. 2008

[3]A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.). Sara Rapson,Man Wu,Shoko Okada,Alpana Das,Pushkar Shrestha,Xue-Rong Zhou,Craig Wood,Allan Green,Surinder Singh,Qing Liu. 2015

[4]Identification of Sugarcane White Leaf Phytoplasma in Fields and Quaratine Sugarcane Samples in Yunnan Province, China. Wang, Xiao-Yan,Li, Wen-Feng,Huang, Ying-Kun,Zhang, Rong-Yue,Shan, Hong-Li,Luo, Zhi-Ming,Yin, Jiong,Shen, Ke. 2015

[5]Characterization of the complete mitochondrial genome of Oxygymnocypris stewartii (Cypriniformes: Cyprinidae). Ji, Wei,Zhang, Gui-Rong,Wei, Kai-Jian,Guo, Shan-Shan,Guo, Xiang-Zhao,Ji, Wei,Zhang, Gui-Rong,Wei, Kai-Jian,Guo, Shan-Shan,Guo, Xiang-Zhao,Wei, Qi-Wei. 2015

[6]Phylogenetic analysis of Babesia species in China based on cytochrome b (COB) gene. Tian, Zhancheng,Luo, Jin,Zheng, Jinfeng,Xie, Junren,Shen, Hui,Yin, Hong,Luo, Jianxun,Tian, Meiyuan,Yuan, Xiaosong,Wang, Fangfang,Liu, Guangyuan. 2013

[7]A genomic perspective on the important genetic mechanisms of upland adaptation of rice. Lyu, Jun,Gou, Zhiheng,Wang, Wen,Li, Baoye,Zhang, Shilai,Zhang, Jing,Tao, Dayun,Huang, Wangqi,Hu, Fengyi,He, Weiming,Meng, Liyun,Li, Xin. 2014

[8]The complete mitogenome sequence of Ptychobarbus dipogon (Cypriniformes: Cyprinidae). Zhang, Gui-Rong,Ji, Wei,Wei, Kai-Jian,Guo, Xiang-Zhao,Xie, Cong-Xin,Zhang, Gui-Rong,Ji, Wei,Wei, Kai-Jian,Guo, Xiang-Zhao,Shi, Ze-Chao,Wei, Qi-Wei. 2015

[9]Phylogeny and Evolution of Multiple Common Carp (Cyprinus carpio L.) Populations Clarified by Phylogenetic Analysis Based on Complete Mitochondrial Genomes. Dong, Chuanju,Xu, Jian,Wang, Baosen,Sun, Xiaowen,Xu, Peng,Dong, Chuanju,Xu, Jian,Wang, Baosen,Sun, Xiaowen,Xu, Peng,Dong, Chuanju,Feng, Jianxin,Jeney, Zsigmond.

[10]Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. Li, Shengben,Fu, Zhiming,Zeng, Dali,Meng, Xiangbing,Zhang, Jian,Li, Jiayang,Wang, Yonghong,Li, Shengben,Fu, Zhiming,Zeng, Dali,Meng, Xiangbing,Zhang, Jian,Li, Jiayang,Wang, Yonghong,Qian, Qian,Zeng, Dali,Zhu, Xudong,Kyozuka, Junko,Maekawa, Masahiko.

[11]Prevalence of Theileria infections in goats and sheep in southeastern China. Yin, Hong.

[12]A novel maternal lineage revealed in sheep (Ovis aries). Guo, J,Du, LX,Ma, YH,Guan, WJ,Li, HB,Zhao, QJ,Li, X,Rao, SQ. 2005

[13]A Ser-Gly substitution in plastid-encoded photosystem II D1 protein is responsible for atrazine resistance in foxtail millet (Setaria italica). Jia, Xiaoping,Yuan, Jincheng,Shi, Yunsu,Song, Yancun,Wang, Guoying,Wang, Tianyu,Li, Yu.

[14]Sequence analysis of four caprine mitochondria DNA lineages. Wu, Yan-Ping,Guan, Wei-Jun,Ma, Yue-Hui,Wu, Yan-Ping,Huo, Jun-Hong,Xie, Jin-Fang. 2012

[15]Genetic deviation in geographically close populations of the dengue vector Aedes aegypti (Diptera: Culicidae): influence of environmental barriers in South India. Vadivalagan, Chithravel,Murugan, Kadarkarai,Panneerselvam, Chellasamy,Paulpandi, Manickam,Madhiyazhagan, Pari,Dinesh, Devakumar,Karthika, Pushparaj,Wei, Hui,Aziz, Al Thabiani,Alsalhi, Mohamad Saleh,Devanesan, Sandhanasamy,Nicoletti, Marcello,Paramasivan, Rajaiah,Benelli, Giovanni.

[16]Phylogenetic relationships of wild roses in China based on nrDNA and matK data. Qiu, Xianqin,Zhang, Hao,Wang, Qigang,Jian, Hongying,Yan, Huijun,Zhang, Ting,Wang, Jihua,Tang, Kaixue.

[17]Genetic diversity and phylogeny of tea plant (Camellia sinensis) and its related species and varieties in the section Thea genus Camellia determined by randomly amplified polymorphic DNA analysis. Chen, L,Yamaguchi, S.

[18]Molecular detection of high- and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. H. Jin,J. Yan,R. J. Peña,X. C. Xia,A. Morgounov,L. M. Han,Y. Zhang,Z. H. He. 2011

[19]A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. Liu, Li,Wang, Aili,Ma, Junhong,Lan, Ping,Ma, Wujun,Liu, Li,Xia, Xianchun,He, Zhonghu,Wang, Aili,Yan, Yueming,He, Zhonghu,Bekes, Frank,Appels, Rudi. 2009

[20]Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci. Dai, Shoufen,Liu, Dengcai,Jiang, Jiaqi,Wei, Yuming,Wu, Bihua,Lan, Xiujin,Zheng, Youliang,Yan, Zehong,Pu, Zhien,Pu, Zongjun. 2013

作者其他论文 更多>>

微信小程序