文献类型： 外文期刊
第一作者： Shi, Pengjun
作者： Shi, Pengjun;Du, Yanlong;Yang, Hong;Huang, Huoqing;Wang, Yaru;Yao, Bin;Zhang, Xiu
作者机构：
期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）
ISSN： 2314-6133
年卷期： 2015 年
页码：
收录情况： SCI
摘要： An endo-1,4-beta-xylanase-encoding gene, xyn11B, was cloned fromthe thermophilic fungus Humicola insolensY1. The gene encodes a multimodular xylanase that consists of a typical hydrophobic signal sequence, a catalytic domain of glycoside hydrolase (GH) family 11, a glycine-rich linker, and a family 1 carbohydrate binding module (CBM1). Deduced Xyn11B shares the highest identity of 74% with a putative xylanase from Podospora anserina S mat+. Recombinant Xyn11B was successfully expressed in Pichia pastoris and purified to electrophoretic homogeneity. Xyn11B had a high specific activity of 382.0 U mg(-1) towards beechwood xylan and showed optimal activity at pH 6.0 and 50 degrees C. Distinct from most reported acidic fungal xylanases, Xyn11B was alkaline-tolerant, retaining 30.7% of the maximal activity at pH 9.0. The K-m and K-max values for beechwood xylanwere 2.2 mg mL(-1) and 462.8 mu mol min(-1) mg(-1), respectively. The enzyme exhibited a wider substrate specificity and produced a mixture of xylooligosaccharides. All these favorable enzymatic properties make Xyn11B attractive for potential applications in various industries.
分类号：
- 相关文献
作者其他论文 更多>>
-
Fluorescent Probes Based on Ag NPs@N/GQDs and Molecularly Imprinted Polymer for Sensitive Detection of Noradrenaline in Bananas
作者：Wang, Yaru;Wang, Mingyue;Liu, Sixin;Wang, Yaru;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Wu, Yuwei;Wang, Mingyue;Wang, Yaru;Li, Shuhuai;Wang, Mingyue;Li, Bei
关键词：Fluorescent probe; Graphene quantum dots; Silver nanoparticles; Molecular imprinted polymer; Noradrenaline
-
Single nucleotide polymorphisms in the MRFs gene family associated with growth in Nile tilapia
作者：Wei, Longjie;Xiao, Wei;Zou, Zhiying;Zhu, Jinglin;Yang, Hong;Xiao, Wei;Chen, Binglin;Zou, Zhiying;Zhu, Jinglin;Li, Dayu;Yu, Jie;Yang, Hong
关键词：Nile tilapia; Myogenic regulatory factors gene family; Growth traits; Diplotype
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者：Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词：high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者：Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词：
-
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
作者：He, Jinjian;Li, Yuying;Sun, Xihang;Zuo, Dinghui;Wang, Mansheng;Zheng, Xia;Shi, Pengjun;Zuo, Dinghui;Yu, Pinglian
关键词：beta-glucosidase; Acidilobus sp.; thermophilic; thermostability; ethanol tolerance; isoflavone glycosides
-
Genome-wide characterization and expression of Oryza sativa AP2 transcription factor genes associated with the metabolism of mesotrione
作者：Chen, Zhao Jie;Shi, Xu Zhen;He, Zhi Hai;Qu, Ya Nan;Wang, Yi Zhuo;Yang, Hong;Ai, Gan;Wang, Yan Hui
关键词：AP2 transcription factor; Mesotrione; Rice; Bioinformatics analysis; Metabolism
-
Comparative assessment of morphological, cytological, and reproductive characteristics of the spontaneous mixoploid 'MM 2 x /4 x' and its diploid counterpart 'Miss Manilla ' in Bougainvillea ( Bougainvillea x buttiana Honlttum & Standl.)
作者：Li, Haiyan;Leng, Qingyun;Sun, Luping;Lu, Jinping;Xu, Shisong;Wang, Yaru;Liu, Shaoshan;Yin, Junmei;Niu, Junhai;Li, Haiyan;Wang, Yaru;Yin, Junmei;Niu, Junhai;Leng, Qingyun;Wang, Yaru;Niu, Junhai;Yin, Junmei;Lopez, Hernan Ariel;Lopez, Hernan Ariel;Ma, Chao
关键词：Bougainvillea; Mixoploid; Morphological traits; Cytological analysis; Hybridization; Polyploid breeding
