Effects of inter-culture, arabinogalactan proteins, and hydrogen peroxide on the plant regeneration of wheat immature embryos

文献类型： 外文期刊

第一作者： Zhang Wei

作者： Zhang Wei;Wang Xin-min;Yin Gui-xiang;Wang Ke;Du Li-pu;Xiao Le-le;Ye Xing-guo;Fan Rong

作者机构：

关键词： wheat;immature embryos;plant regeneration;inter-culture;arabinogalactan proteins;hydrogen peroxide

期刊名称：Journal of Integrative Agriculture （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： The regeneration rate of wheat immature embryo varies among genotypes, howbeit many elite agriculture wheat varieties have low regeneration rates. Optimization of tissue culture conditions and attempts of adding signal molecules are effective ways to increase plant regeneration rate. Inter-culture is one of ways that have not been investigated in plant tissue culture. Moreover, the use of arabinogalactan proteins (AGPs) and hydrogen peroxide (H2O2) have been reported to increase regeneration rate in a few plant species other than wheat. The current research pioneeringly uses inter-culture of immature embryos of different wheat genotypes, and also investigates impacts of AGP and H2O2 on the induction of embryogenic calli and plant regeneration. As a result, high-frequency regeneration wheat cultivars Kenong 199 (KN199) and Xinchun 9 (XC9), together with low-frequency regeneration wheat line Chinese Spring (CS), presented striking increase in the induction of embryogenic calli and plant regeneration rate of CS through inter-culture strategy, up to 52.19 and 67.98%, respectively. Adding 50 to 200 mg L-1 AGP or 0.005 to 0.01 parts per thousand H2O2 to the callus induction medium, enhanced growth of embryogenic calli and plant regeneration rate in quite a few wheat genotypes. At 50 mg L-1 AGP application level in callus induction medium plant regeneration rates of 8.49, 409.06 and 283.16% were achieved for Jimai 22 (JM22), Jingdong 18 (JD18) and Yangmai 18 (YM18), respectively; whereas at 100 mg L-1 AGP level, CS (105.44%), Chuannong 16 (CN16) (80.60%) and Ningchun 4 (NC4) (62.87%) acted the best. Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 parts per thousand of H2O2, and NC4 (35.37%) obtained the highest shoot regeneration rates at 0.01 parts per thousand of H2O2. Overall, these two methods, inter-culture and AGP (or H2O2) application, can be further applied to wheat transgenic research.

分类号：

  • 相关文献

[1]Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.). Wang Xin-min,Yin Gui-xiang,Wang Ke,Du Li-pu,Xu Hui-jun,Ye Xing-guo,Ren Xian,Li Jia-rui. 2014

[2]Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens. Tao Li-li,Yin Gui-xiang,Du Li-pu,Shi Zheng-yuan,She Mao-yun,Xu Hui-jun,Ye Xing-guo. 2011

[3]EFFECTS OF SOIL DROUGHT STRESS ON PLANT REGENERATION EFFICIENCY AND ENDOGENOUS HORMONE LEVELS OF IMMATURE EMBRYOS IN WHEAT (&ITTRITICUM AESTIVUM&IT L.). Bie, Xiaomin,Wang, Ke,Liu, Chang,Du, Lipu,Mao, Xinguo,Ye, Xingguo,Bie, Xiaomin,Liu, Yongwei. 2017

[4]Improvement of isolated microspore culture of barley (Hordeum vulgare L.): the effect of floret co-culture. Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Chen, Peidu.

[5]Cadmium stress tolerance in wheat seedlings induced by ascorbic acid was mediated by NO signaling pathways. Wang, Zhaofeng,Guo, Jie,Yang, Yingli,Li, Qien,Wu, Weiguo.

[6]Variable content and distribution of arabinogalactan proteins in banana (Musa spp.) under low temperature stress. Chen, Houbin,Wang, Yingying,Xu, Enfeng,Xie, Ling,Su, Zhaohua,Xu, Chunxiang,Takac, Tomas,Samaj, Jozef,Li, Xiaoquan. 2015

[7]Genome-wide identification, classification and expression analysis of genes encoding putative fasciclin-like arabinogalactan proteins in Chinese cabbage (Brassica rapa L.). Li Jun,Wu Xiaoming.

[8]The putative phytocyanin genes in Chinese cabbage (Brassica rapa L.): genome-wide identification, classification and expression analysis. Li, Jun,Gao, Guizhen,Zhang, Tianyao,Wu, Xiaoming.

[9]Comparison of dynamic changes of endogenous hormones between calli derived from mature and immature embryos of maize. Wang, Henning,Zhang, Jinwen,Jia, Xiaoxia,Liang, Huiguang,Kong, Weiping,Zhang, Xinhui. 2011

[10]Somatic embryogenesis from thin epidermal layers in sunflower and chromosomal regions controlling the response. Huang, XianQun,Nabipour, Alireza,Gentzbittel, Laurent,Sarrafi, Ahmad.

[11]Plant regeneration via somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Chang-Bing Yao. 2000

[12]Highly efficient plant regeneration through somatic embryogenesis in 20 elite commercial cotton (Gossypium hirsutum L.) cultivars. Baohong Zhang,Qinglian Wang,Fang Liu,Kunbo Wang,Taylor P. Frazier. 2009

[13]Plant regeneration system from cotyledons-derived calluses cultures of Stylosanthes guianensis cv. 'Reyan 2'. Yuan, Xuejun,Liu, Guodao,Yuan, Xuejun,Wang, Zhiyong,Liao, Li,Wang, Zhiyong. 2011

[14]Plant regeneration from cotyledon of Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How). He, Xiaoming,Xie, Dasen,Peng, Qingwu,Mu, Lixia. 2007

[15]Plant regeneration from cell suspension-derived protoplasts of Populus x beijingensis. Cai, Xiao,Kang, Xiang-Yang,Cai, Xiao. 2014

[16]Direct somatic embryogenesis from leaf and petiole explants of Spathiphyllum 'Supreme' and analysis of regenerants using flow cytometry. Zhao, Jietang,Henny, Richard J.,Chen, Jianjun,Cui, Jin,Liu, Juanxu,Liao, Feixiong,Chen, Jianjun. 2012

[17]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[18]Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton via Efficient Selection and Timely Subculture of Somatic Embryos. Wu, Shen-Jie,Wang, Hai-Hai,Li, Fei-Fei,Chen, Tian-Zi,Zhang, Jie,Jiang, Yan-Jie,Ding, Yezhang,Guo, Wang-Zhen,Zhang, Tian-Zhen,Wu, Shen-Jie.

[19]Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis). Sheng, Xiaoguang,Zhao, Zhenqing,Yu, Huifang,Wang, Jiansheng,Xiaohui, Zhang,Gu, Honghui. 2011

[20]High-Efficiency Plant Regeneration from Immature Inflorescence Derived Callus Cultures of Two Phenotypically Distinct Accessions of Centipedegrass (Eremochloa ophiuroides). Ma, Jingjing,Wang, Yi,Zong, Junqin,Zhang, Bing,Chen, Jingbo,Li, Dandan,Li, Ling,Guo, Hailin,Liu, Jianxiu,Li, Jianjian,She, Jianming. 2018

作者其他论文 更多>>

微信小程序