Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in Brassica oleracea

文献类型： 外文期刊

第一作者： Lv, Honghao

作者： Lv, Honghao;Fang, Zhiyuan;Yang, Limei;Zhang, Yangyong;Liu, Yumei;Zhuang, Mu;Yang, Yuhong;Xie, Bingyan;Liu, Bo;Liu, Jisheng;Wang, Xiaowu;Wang, Qingbiao;Kang, Jungen

作者机构：

关键词： Brassica oleracea;Fusarium wilt;Resistance gene;FOC1;Map-based cloning

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2014 年 15 卷

页码：

收录情况： SCI

摘要： Background: Cabbage Fusarium wilt is a major disease worldwide that can cause severe yield loss in cabbage (Brassica olerecea). Although markers linked to the resistance gene FOC1 have been identified, no candidate gene for it has been determined so far. In this study, we report the fine mapping and analysis of a candidate gene for FOC1 using a double haploid (DH) population with 160 lines and a F-2 population of 4000 individuals derived from the same parental lines. Results: We confirmed that the resistance to Fusarium wilt was controlled by a single dominant gene based on the resistance segregation ratio of the two populations. Using InDel primers designed from whole-genome re-sequencing data for the two parental lines (the resistant inbred-line 99-77 and the highly susceptible line 99-91) and the DH population, we mapped the resistance gene to a 382-kb genomic region on chromosome C06. Using the F2 population, we narrowed the region to an 84-kb interval that harbored ten genes, including four probable resistance genes (R genes): Bol037156, Bol037157, Bol037158 and Bol037161 according to the gene annotations from BRAD, the genomic database for B. oleracea. After correcting the model of the these genes, we re-predicted two R genes in the target region: re-Bol037156 and re-Bol0371578. The latter was excluded after we compared the two genes' sequences between ten resistant materials and ten susceptible materials. For re-Bol037156, we found high identity among the sequences of the resistant lines, while among the susceptible lines, there were two types of InDels (a 1-bp insertion and a 10-bp deletion), each of which caused a frameshift and terminating mutation in the cDNA sequences. Further sequence analysis of the two InDel loci from 80 lines (40 resistant and 40 susceptible) also showed that all 40 R lines had no InDel mutation while 39 out of 40 S lines matched the two types of loci. Thus re-Bol037156 was identified as a likely candidate gene for FOC1 in cabbage. Conclusions: This work may lay the foundation for marker-assisted selection as well as for further function analysis of the FOC1 gene.

分类号：

  • 相关文献

[1]Development of InDel markers linked to Fusarium wilt resistance in cabbage. Lv, Hong-hao,Yang, Li-mei,Wang, Qing-biao,Wang, Xiao-wu,Fang, Zhi-yuan,Liu, Yu-mei,Zhuang, Mu,Zhang, Yang-yong,Lin, Yan,Yang, Yu-hong,Xie, Bing-yan,Liu, Bo,Liu, Ji-Sheng,Kang, Jun-gen.

[2]A B-lectin receptor kinase gene conferring rice blast resistance. Chen, Xuewei,Shang, Junjun,Chen, Dexi,Lei, Cailin,Zou, Yan,Zhai, Wenxue,Liu, Guozhen,Xu, Jichen,Ling, Zhongzhuan,Cao, Gang,Ma, Bingtian,Wang, Yuping,Zhao, Xianfeng,Li, Shigui,Zhu, Lihuang. 2006

[3]Diversity of Sicilian broccoli (Brassica oleracea var. italica) and cauliflower (Brassica oleracea var. botrytis) landraces and their distinctive bio-morphological, antioxidant, and genetic traits. Branca, Ferdinando,Chiarenza, Giuseppina Laura,Cavallaro, Chiara,Tribulato, Alessandro,Gu, Honghui,Zhao, Zhenqing. 2018

[4]Genetic and Epigenetic Alterations of Brassica nigra Introgression Lines from Somatic Hybridization: A Resource for Cauliflower Improvement. Wang, Gui-xiang,Lv, Jing,Zhang, Jie,Han, Shuo,Zong, Mei,Guo, Ning,Zeng, Xing-ying,Zhang, Yue-yun,Liu, Fan,Lv, Jing,Wang, You-ping,Lv, Jing. 2016

[5]Inheritance and InDel markers closely linked to petal color gene (cpc-1) in Brassica oleracea. Han, Feng-qing,Yang, Chong,Fang, Zhi-yuan,Yang, Li-mei,Zhuang, Mu,Lv, Hong-hao,Liu, Yu-mei,Li, Zhan-sheng,Liu, Bo,Yu, Hai-long,Liu, Xiao-ping,Zhang, Yang-yong.

[6]Genetic analysis and QTL mapping of traits related to head shape in cabbage (Brassica oleracea var. capitata L.). Mang, Xiaoli,Liu, Yumei,Fang, Zhiyuan,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Li, Zhansheng,Lv, Honghao,Su, Yanbin.

[7]Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray. Bonnema, Guusje,Kang, Jungen,Zhang, Guoyu,Fang, Zhiyuan,Wang, Xiaowu.

[8]Identification of tapetum-specific genes by comparing global gene expression of four different male sterile lines in Brassica oleracea. Ma, Yuan,Zhu, Yingguo,Ma, Yuan,Wu, Jian,Wang, Xiaowu,Kang, Jungen.

[9]Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var. capitata L.. Lv, Honghao,Wang, Qingbiao,Zhang, Yangyong,Yang, Limei,Fang, Zhiyuan,Wang, Xiaowu,Liu, Yumei,Zhuang, Mu,Lin, Yan,Yu, Hailong,Liu, Bo.

[10]Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line. Han, Fengqing,Zhang, Xiaoli,Liu, Xing,Su, Henan,Kong, Congcong,Fang, Zhiyuan,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Liu, Yumei,Li, Zhansheng,Lv, Honghao,Han, Fengqing,Liu, Xing. 2017

[11]Bolbase: a comprehensive genomics database for Brassica oleracea. Yu, Jingyin,Zhao, Meixia,Tong, Chaobo,Huang, Shunmou,Tehrim, Sadia,Hua, Wei,Liu, Shengyi,Wang, Xiaowu,Liu, Yumei,Zhao, Meixia. 2013

[12]Assessing and broadening genetic diversity of a rapeseed germplasm collection. Wu, Jinfeng,Li, Feng,Xu, Kun,Cao, Guizhen,Chen, Biyun,Yan, Cuixin,Wang, Nian,Qiao, Jiangwei,Li, Jun,Li, Hao,Zhang, Tianyao,Song, Weiling,Wu, Xiaoming.

[13]Cytogenetic analysis of F-1, F-2 and BC, plants from intergeneric sexual hybridization between Sinapis alba and Brassica oleracea by genomic in situ hybridization. Wei, W. H.,Zhang, S. F.,Wang, L. J.,Li, J.,Chen, B.,Wang, Z.,Luo, L. X.,Fang, X. P.. 2007

[14]Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes. Kong, Fang,Ge, Cailin,Wang, Youping,Fang, Xiaoping,Snowdon, Rod J.. 2010

[15]Study on the Relationship Between the Ploidy Level of Microspore-Derived Plants and the Number of Chloroplast in Stomatal Guard Cells in Brassica oleracea. Yuan Su-xia,Liu Yu-mei,Fang Zhi-yuan,Yang Li-mei,Zhuang Mu,Zhang Yang-yong,Sun Pei-tian. 2009

[16]Development and Validation of High-glucoraphanin Broccoli F-1 Hybrids and Parental Lines. Gu, Honghui,Wang, Jiansheng,Yu, Huifang,Zhao, Zhenqing,Sheng, Xiaoguang,Chen, Jisuan,Xu, Yingjun. 2014

[17]Analysis of intergeneric sexual hybridization between transgenic Brassica oleracea and Sinapis alba. Li, Jun,Wei, Wenhui,Lu, Guangyuan,Li, Jun,Zhang, Chunlei,Luo, Lixia,Ren, Li,Fang, Xiaoping,Guan, Chunyun,Lu, Guangyuan.

[18]Genetics and fine mapping of a yellow-green leaf gene (ygl-1) in cabbage (Brassica oleracea var. capitata L.). Liu, Xiao-ping,Yang, Chong,Han, Feng-qing,Fang, Zhi-yuan,Yang, Li-mei,Zhuang, Mu,Lv, Hong-hao,Liu, Yu-mei,Li, Zhan-sheng,Zhang, Yang-yong,Yang, Chong.

[19]Heritable variation and small RNAs in the progeny of chimeras of Brassica juncea and Brassica oleracea. Li, Junxing,Wang, Yan,Zhang, Langlang,Liu, Bin,Cao, Liwen,Qi, Zhenyu,Chen, Liping,Li, Junxing,Zhang, Langlang,Liu, Bin,Cao, Liwen,Chen, Liping,Wang, Yan,Qi, Zhenyu.

[20]Transcript profiling of a dominant male sterile mutant (Ms-cd1) in cabbage during flower bud development. Lou, Ping,Kang, Jungen,Zhang, Guoyu,Bonnema, Guusje,Fang, Zhiyuan,Wang, Xiaowu. 2007

作者其他论文 更多>>

微信小程序