Simultaneous determination of toltrazuril and its metabolites in chicken and pig skin plus fat by UPLC-UV method

文献类型: 外文期刊

第一作者: Zheng, Wenli

作者: Zheng, Wenli;Jiang, Zhaoling;Zhang, Lifang;Zhang, Chong;Zhang, Xiao;Fei, Chenzhong;Zhang, Keyu;Wang, Xiaoyang;Wang, Mi;Li, Tao;Xiao, Sui;Wang, Chunmei;Xue, Feiqun

作者机构:

关键词: Toltrazuril;Metabolites;Skin plus fat;Residues;UPLC-UV

期刊名称:JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ( 影响因子:3.205; 五年影响因子:3.068 )

ISSN: 1570-0232

年卷期: 2014 年 972 卷

页码:

收录情况: SCI

摘要: A reliable method for the simultaneous determination of toltrazuril and its main metabolites (toltrazuril sulphone and toltrazuril sulphoxide) in chicken and pig skin + fat was developed and validated. Analytes were extracted from skin + fat with acetonitrile. The crude extracts were subjected to liquid-liquid extraction with n-hexane, and then further cleaned using primary secondary amine and Oasis (TM) MAX solid phase extraction cartridges. Chromatographic separation by UPLC-UV was performed on a C18+ reversed-phase column with gradient elution. Relative recovery from the spiked samples ranged from 84.8% to 109.1%. Limits of detection and quantification for the analytes were within 25-37.5 mu g kg(-1) and 50-75 mu g kg(-1), respectively. The developed method has been successfully applied to the depletion study of toltrazuril drug residues in chicken skin + fat. The recommended withdrawal period with oral administration based on our research is 24.18 days. (C) 2014 Elsevier B.V. All rights reserved.

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