Label-Free and Sensitive Fluorescent Detection of Sequence-Specific Single-Strand DNA Based on S1 Nuclease Cleavage Effects

文献类型： 外文期刊

第一作者： Guan, Zheng

作者： Guan, Zheng;Liu, Jinchuan;Bai, Wenhui;Lv, Zhenzhen;Jiang, Xiaoling;Yang, Shuming;Chen, Ailiang;Guan, Zheng;Liu, Jinchuan;Bai, Wenhui;Lv, Zhenzhen;Jiang, Xiaoling;Yang, Shuming;Chen, Ailiang;Guan, Zheng;Lv, Guiyuan

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 10 期

页码：

收录情况： SCI

摘要： The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic. Here, we have demonstrated a simple label-free sensing strategy to detect ssDNA by employing its complementary ssDNA, S1 nuclease and nucleic acid fluorescent dyes. Upon clearing away redundant complementary ssDNA and possibly mismatched double strand DNA by using S1 nuclease, the fluorescent signal-to-noise ratio could be increased dramatically. It enabled the method to be adaptable to three different types of DNA fluorescent dyes and the ability to detect target ssDNA in complex, multicomponent samples, like tissue homogenate. The method can distinguish a two-base mismatch from avian influenza A (H1N1) virus. Also, it can detect the appearance of 50 pM target ssDNA in 0.5 mu g.mL(-1) Lambda DNA, and 50 nM target ssDNA in 5 mu g.mL(-1) Lambda DNA or in tissue homogenate. It is facile and cost-effective, and could be easily extended to detect other ssDNA with many common nucleic acid fluorescent dyes.

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