Detection of Melamine in Feed Using Liquid-Liquid Extraction Treatment Combined with Surface-Enhanced Raman Scattering Spectroscopy

文献类型： 外文期刊

第一作者： Cheng, Jie

作者： Cheng, Jie;Wang, Shi;Su, Xiao-Ou

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 9 期

页码：

收录情况： SCI

摘要： A rapid, selective, and sensitive method to determine the melamine content in animal feeds was developed using surface-enhanced Raman scattering spectroscopy on aggregated 55 nm Au nanoparticles with liquid-liquid extraction sample preparation. Butyl alcohol was used as the initial extraction solvent, and liquid-liquid extraction was performed twice using HCl (pH 3-4) and 6:1 (v/v) n-butyl alcohol/ethyl acetate. The intensity of the matrix-based peak at 731 cm(-1) was set at 100 as a basis for the feeds, and the peak at 707 cm(-1) was the characteristic peak of melamine used in the calculations. Sufficient linearity was obtained in the range 2-10 mu g.g(-1) (R-2 = 0.991). Limits of detection and quantification in the feeds were 0.5 and 2 mu g.g(-1), respectively. The recovery rates were 82.5-90.2% with coefficients of variation below 4.02%. This new protocol could be easily developed for the routine monitoring of on-site feed quality and market surveillance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序