Protective efficacy of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) adjuvated with recombinant IL-15 and IL-21 against experimental toxoplasmosis in mice

文献类型： 外文期刊

第一作者： Chen, Jia

作者： Chen, Jia;Li, Zhong-Yuan;Huang, Si-Yang;Song, Hui-Qun;Zhou, Dong-Hui;Zhu, Xing-Quan;Chen, Jia;Zhu, Xing-Quan;Petersen, Eskild;Petersen, Eskild

作者机构：

关键词： Toxoplasma gondii;Toxoplasmosis;TgCDPK1;pVAX-IL-15-IL-21;DNA vaccine;Protective immunity

期刊名称：BMC INFECTIOUS DISEASES （ 影响因子：3.09； 五年影响因子：3.401 ）

ISSN： 1471-2334

年卷期： 2014 年 14 卷

页码：

收录情况： SCI

摘要： Background: Toxoplasma gondii can infect all warm-blooded animals including humans. Infection with T. gondii is probably the leading cause of posterior uveitis in humans and the most comment route of transmission is raw and undercooked meat from infected animals. T. gondii calcium-dependent protein kinase 1 (TgCDPK1) plays a critical role in direct parasite motility, host-cell invasion, and egress. Methods: We constructed a DNA vaccine expressing TgCDPK1 inserted into eukaryotic expression vector pVAX I and evaluated the immune protection induced by pVAX-CDPK1 in Kunming mice. Mice immunized with pVAX-CDPK1 intramuscularly and/or with a plasmid encoding IL-15 and IL-21 (pVAX-IL-21-IL-15). The immune responses were analyzed including lymphoproliferative assay, cytokine, antibody measurements, lymphocyte surface markers by flow cytometry and protective efficacy were measured as survival and cysts numbers after challenge 1 to 2 months post vaccination. Results: Immunization with pVAX-CDPK1 or pVAX-IL-21-IL-15 alone developed strong humoral responses and Th1 type cellular immune responses, and the significantly (P < 0.05) increase of both the percentages of CD4+ and CD8 + T cells compared with all the controls (blank control, PBS, and pVAX). Co-injection of pVAX-IL-21-IL-15 significantly increased humoral and cellular immune responses compared to the group of pVAX-CDPK1 or pVAX-IL-21-IL-15. Challenge experiments showed that co-administration of pVAX-IL-21-IL-15 and pVAX-CDPK1 significantly (P < 0.05) increased survival time (19.2 +/- 5.1 days) compared with pVAX-CDPK1 (17.3 +/- 4.3 days) or pVAX-IL-21-IL-15 (12.0 +/- 2.0 days) alone, and pVAX-IL-21-IL-15 + pVAX-CDPK1 significantly reduced the number of brain cysts (72.7%) in contrast to pVAX-ROP13 (45.7%) or pVAX-IL-21-IL-15 alone (43.6%). Conclusions: TgCDPK1 is identified to be a promising vaccine candidate for inducing a strong humoral and cellular response against T. gondii infection, and thus synergistic of mIL-21 and mIL-15 can induce non-specific immune responses, but also facilitate specific humoral as well as cellular immune responses elicited by DNA vaccine against acute and chronic T. gondii infection in mice.

分类号：

  • 相关文献

[1]Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii. Chen, Jia,Li, Zhong-Yuan,Liu, Wen-Ge,Zhu, Xing-Quan,Chen, Jia,Petersen, Eskild,Zhu, Xing-Quan.

[2]Protective immunity induced by a DNA vaccine expressing eIF4A of Toxoplasma gondii against acute toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Li, Zhong-Yuan,Yuan, Zi-Guo,Zhou, Dong-Hui,Zhang, Nian-Zhang,Zhu, Xing-Quan,Chen, Jia,Li, Zhong-Yuan,Yuan, Zi-Guo,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[3]Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice. Li, Zhong-Yuan,Chen, Jia,Zhou, Dong-Hui,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild.

[4]DNA vaccination with genes encoding Toxoplasma gondii antigens ROP5 and GRA15 induces protective immunity against toxoplasmosis in Kunming mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Zhou, Dong-Hui,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild,Zhu, Xing-Quan. 2015

[5]DNA immunization with eukaryotic initiation factor-2 alpha of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Zhou, Dong-Hui,Li, Zhong-Yuan,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[6]Protective immunity against Toxoplasma gondii induced by DNA immunization with the gene encoding a novel vaccine candidate: calcium-dependent protein kinase 3. Zhang, Nian-Zhang,Huang, Si-Yang,Zhou, Dong-Hui,Chen, Jia,Zhu, Xing-Quan,Xu, Ying,Zhu, Xing-Quan,Tian, Wei-Peng,Lu, Jing,Zhu, Xing-Quan. 2013

[7]Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2). Chen, Kai,Wang, Jin-Lei,Huang, Si-Yang,Yang, Wen-Bin,Zhu, Wei-Ning,Zhu, Xing-Quan,Huang, Si-Yang,Zhu, Xing-Quan,Yang, Wen-Bin,Zhu, Wei-Ning. 2017

[8]Evaluation of protective immunity induced by DNA vaccination with genes encoding Toxoplasma gondii GRA17 and GRA23 against acute toxoplasmosis in mice. Zhu, Wei-Ning,Wang, Jin-Lei,Chen, Kai,Yue, Dong-Mei,Zhang, Xiao-Xuan,Huang, Si-Yang,Zhu, Xing-Quan,Zhu, Wei-Ning. 2017

[9]Evaluation of immuno-efficacy of a novel DNA vaccine encoding Toxoplasma gondii rhoptry protein 38 (TgROP38) against chronic toxoplasmosis in a murine model. Xu, Ying,Zhang, Nian-Zhang,Tan, Qi-Dong,Chen, Jia,Lu, Jing,Zhu, Xing-Quan,Xu, Ying,Tan, Qi-Dong,Xu, Qian-Ming,Zhu, Xing-Quan. 2014

[10]Vaccination with a DNA vaccine encoding Toxoplasma gondii ROP54 induces protective immunity against toxoplasmosis in mice. Yang, Wen-Bin,Zhao, Guang-Hui,Yang, Wen-Bin,Zhou, Dong-Hui,Zou, Yang,Chen, Kai,Wang, Jin-Lei,Zhu, Xing-Quan,Liu, Qing,Zhu, Xing-Quan. 2017

[11]Evaluation of protective effect of pVAX-TgMIC13 plasmid against acute and chronic Toxoplasma gondii infection in a murine model. Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Zhou, Yang,Zhu, Xing-Quan,Yuan, Zi-Guo,Ren, Di,Zhang, Xiu-Xiang,Li, Xiu-Zhen,Petersen, Eskild,Petersen, Eskild,Yang, Gui-Lian,Zhu, Xing-Quan. 2013

[12]A long-lasting protective immunity against chronic toxoplasmosis in mice induced by recombinant rhoptry proteins encapsulated in poly (lactide-co-glycolide) microparticles. Xu, Ying,Zhang, Nian-Zhang,Wang, Meng,Dong, Hu,Feng, Sheng-Yong,Guo, Hui-Chen,Zhu, Xing-Quan,Xu, Ying,Zhu, Xing-Quan.

[13]Protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 and rhomboid 5 proteins against acute and chronic toxoplasmosis in mice. Zhang, Nian-Zhang,Xu, Ying,Wang, Meng,Chen, Jia,Huang, Si-Yang,Zhu, Xing-Quan,Xu, Ying,Petersen, Eskild,Huang, Si-Yang,Zhu, Xing-Quan. 2015

[14]DNA vaccination with a gene encoding VP60 elicited protective immunity against rabbit hemorrhagic disease virus. Yuan, Dongwei,Qu, Liandong,Liu, Jiasen,Guo, Dongchun,Jiang, Qian,Lin, Huan,Si, Changde.

[15]Toxoplasma gondii: Protective immunity induced by rhoptry protein 9 (TgROP9) against acute toxoplasmosis. Chen, Jia,Zhou, Dong-Hui,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Li, Zhong-Yuan,Zhu, Xing-Quan. 2014

[16]Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations. Ning, H. R.,Zhu, X. Q.,Ning, H. R.,Wang, J. L.,Qin, S. Y.,Huang, S. Y.,Lou, Z. L.,Hu, L. Y.,Zhu, X. Q.,Qin, S. Y.,Lou, Z. L.,Huang, S. Y.,Zhu, X. Q.. 2015

[17]Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii. Xu, Min-Jun,Zhou, Dong-Hui,Huang, Si-Yang,Fan, Yi-Fan,Zhu, Xing-Quan,Nisbet, Alasdair J.,Zhu, Xing-Quan,Fan, Yi-Fan. 2013

[18]Sequence variation in Toxoplasma gondii MIC13 gene among isolates from different hosts and geographical locations. Ren, Di,Zhou, Yang,Lin, Ze-Ping,Lin, Rui-Qing,Wu, Song-Ming,Lin, Shao-Qi,Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Xu, Min-Jun,Zhou, Yang,Wu, Song-Ming,Yuan, Zi-Guo,Yang, Jian-Fa,Zou, Feng-Cai. 2012

[19]Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Jiang, Hai-Hai,Huang, Si-Yang,Zhou, Dong-Hui,Zhang, Xiao-Xuan,Su, Chunlei,Zhu, Xing-Quan,Deng, Shun-Zhou,Su, Chunlei,Zhang, Xiao-Xuan. 2013

[20]Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. Zhao, Yu,Sun, Xiaolin,Zhao, Yu,Zhou, Donghui,Chen, Jia. 2017

作者其他论文 更多>>

微信小程序