Functional Characterization of Chemosensory Proteins in the Scarab Beetle, Holotrichia oblita Faldermann (Coleoptera: Scarabaeida)

文献类型： 外文期刊

第一作者： Guan, Li

作者： Guan, Li;Feng, Honglin;Yin, Jiao;Cao, Yazhong;Li, Kebin;Sun, Hongyan;Guan, Li;Feng, Honglin;Xi, Jinghui

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 9 期

页码：

收录情况： SCI

摘要： Chemosensory proteins (CSPs) play important roles in chemical communication by insects, as they recognize and transport environmental chemical signals to receptors within sensilla. In this study, we identified HoblCSP1 and HoblCSP2 from a cDNA library of Holotrichia oblita antennae, successfully expressed them in E. coli and purified them by Ni ion affinity chromatography. We then measured the ligand-binding specificities of HoblCSP1 and HoblCSP2 to 50 selected ligands in a competitive binding assay. These results demonstrated that HoblCSP1 and HoblCSP2 have similar ligand-binding spectra. Both proteins displayed the highest affinity for beta-ionone, alpha-ionone and cinnamaldehyde, indicating that they prefer binding to odorants other than sex pheromones. Additionally, immuno-localization revealed that HoblCSP1 is highly concentrated in sensilla basiconica, while HoblCSP2 is specifically localized to sensilla placodea. In conclusion, HoblCSP1 and HoblCSP2 are responsible for binding to general odorants with slightly different specificities due to their different in vivo environments.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序