Differentially-expressed genes in rice infected by Xanthomonas oryzae pv. oryzae relative to a flagellin-deficient mutant reveal potential functions of flagellin in host-pathogen interactions

文献类型： 外文期刊

第一作者： Chen, Huamin

作者： Chen, Huamin;Tian, Fang;He, Chenyang;Leach, Jan E.

作者机构：

关键词： Rice;Differentially-expressed genes (DEGs);Flagellin;Immune response;Xanthomonas oryzae pv. oryzae

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2014 年 7 卷

页码：

收录情况： SCI

摘要： Background: Plants have evolved a sensitive defense response system that detects and recognizes various pathogen-associated molecular patterns (PAMPs) (e.g. flagellin) and induces immune responses to protect against invasion. Transcriptional responses in rice to PAMPs produced by Xanthomonas oryzae pv. oryzae (Xoo), the bacterial blight pathogen, have not yet been defined. Results: We characterized transcriptomic responses in rice inoculated with the wildtype (WT) Xoo and flagellin-deficient mutant Delta fliC through RNA-seq analysis. Digital gene expression (DGE) analysis based on Solexa/Illumina sequencing was used to investigate transcriptomic responses in 30 day-old seedlings of rice (Oryza sativa L. cv. Nipponbare). 1,680 genes were differentially-expressed (DEGs) in rice inoculated with WT relative Delta fliC; among which 1,159 genes were up-regulated and 521 were down-regulated. Expression patterns of 12 randomly-selected DEGs assayed by quantitative real time PCR (qRT-PCR) were similar to those detected by DGE analyses, confirming reliability of the DGE data. Functional annotations revealed the up-regulated DEGs are involved in the cell wall, lipid and secondary metabolism, defense response and hormone signaling, whereas the down-regulated ones are associated with photosynthesis. Moreover, 57 and 21 specifically expressed genes were found after WT and Delta fliC treatments, respectively. Conclusions: DEGs were identified in rice inoculated with WT Xoo relative to Delta fliC. These genes were predicted to function in multiple biological processes, including the defense response and photosynthesis in rice. This study provided additional insights into molecular basis of rice response to bacterial infection and revealed potential functions of bacterial flagellin in the rice-Xoo interactions.

分类号：

  • 相关文献

[1]Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency. Chen Hua-min,Tian Fang,He Chen-Yang,Bi Yong-Mei,Steven, Rothstein J.,Steven, Rothstein J.,Jan, Leach E.. 2015

[2]Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response. Zhang, Fan,Huang, Li-Yu,Zhang, Fan,Zhuo, Da-Long,Li, Zhi-kang,Zhou, Yong-Li,Huang, Li-Yu,Ali, Jauhar,Cruz, Casiana Vera,Du, Zheng-Lin. 2015

[3]XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice. Wang, Chunlian,Zhang, Xiaoping,Fan, Yinglun,Gao, Ying,Zheng, Chongke,Qin, Tengfei,Li, Yanqiang,Che, Jinying,Zhang, Mingwei,Zhao, Kaijun,Zhu, Qinlong,Liu, Yaoguang,Yang, Bing. 2015

[4]Characteristic Expression Analysis of Five WRKY Transcriptional Factors in Rice Leaf Growth and Disease Resistance Reaction. Miao Liu-Yang,Li Li-Yun,Liu Zhao,Liu Yu-Meng,Liu Guo-Zhen,Jiang Guang-Huai,Yang Feng-Huan,He Chen-Yang. 2013

[5]Overlap between Signaling Pathways Responsive to Xanthomonas oryzae pv. oryzae Infection and Drought Stress in Rice Introgression Line Revealed by RNA-Seq. Zhang, Fan,Zhang, Fan,Huang, Liyu,Xu, Jianlong,Zhou, Yongli,Li, Zhikang,Xu, Jianlong,Zhou, Yongli,Li, Zhikang,Huang, Liyu,Cruz, Casiana Vera,Ali, Jauhar,Zhang, Fan.

[6]Rice OsFLS2-Mediated Perception of Bacterial Flagellins Is Evaded by Xanthomonas oryzae pvs. oryzae and oryzicola. Wang, Shanzhi,Sun, Zhe,Wang, Huiqin,Liu, Lijuan,Lu, Fen,Yang, Jun,Guo, Zejian,Sun, Wenxian,Wang, Shanzhi,Sun, Zhe,Wang, Huiqin,Liu, Lijuan,Lu, Fen,Guo, Zejian,Sun, Wenxian,Yang, Jun,Zhang, Shiyong,Zhang, Min,Bent, Andrew F.. 2015

[7]Alternative sigma factor RpoN2 is required for flagellar motility and full virulence of Xanthomonas oryzae pv. oryzae. Yu, Chao,Li, Haiyun,Wu, Xiaoli,Li, Bo,Chen, Huamin,Wu, Maosen,He, Chenyang.

[8]Immunoenhancement with flagellin as an adjuvant to whole-killed rabies vaccine in mice. Xiao, Xing-xing,Zhang, Yun,Liu, Ji-xing,Wei, Qiao-lin,Yin, Xiang-ping.

[9]Host Specificity of Flagellins from Segmented Filamentous Bacteria Affects Their Patterns of Interaction with Mouse Ileal Mucosal Proteins. Chen, Huahai,Wang, Yanling,Xiang, Charlie,Chen, Huahai,Wang, Yanling,Xiang, Charlie,Chen, Huahai,Yin, Yeshi,Wang, Xin,Chen, Huahai,Yin, Yeshi,Wang, Xin. 2017

[10]RpoN2-and FliA-regulated fliTX is indispensible for flagellar motility and virulence in Xanthomonas oryzae pv. oryzae. Chen, Huamin,Tian, Fang,Yang, Fenghuan,He, Chenyang. 2017

[11]Ferric Iron Absorption in Delta rpfFxoo, a Gene Deletion Mutant of Xanthomonas oryzae pv. Oryzae, Assayed Using Atomic Absorption Spectrophotometry. Wu Mao-sen,He Chen-yang. 2010

[12]Mutation of alkyl hydroperoxide reductase gene ahpC of Xanthomonas oryzae pv. oryzae affects hydrogen peroxide accumulation during the rice-pathogen interaction. Qiao, Jiaju,Yang, Lipeng,Li, Xinling,Qiao, Suyu,Li, Xin,Tian, Fang,Chen, Huamin,He, Chenyang,Pang, Xinyue.

[13]Type-II secretion pathway structural gene xpsE, xylanase- and cellulase secretion and virulence in Xanthomonas oryzae pv. oryzae. Sun, QH,Hu, J,Huang, GX,Ge, C,Fang, RX,He, CZ. 2005

[14]The GGDEF-domain protein GdpX1 attenuates motility, exopolysaccharide production and virulence in Xanthomonas oryzae pv. oryzae. Qian, S.,Tian, F.,Chen, H.,He, C.,Hutchins, W.,Yang, C. -H..

[15]Differentiation in MALDI-TOF MS and FTIR spectra between two pathovars of Xanthomonas oryzae. Ge, Mengyu,Li, Bin,Wang, Li,Tao, Zhongyun,Xie, Guanlin,Li, Bin,Mao, Shengfeng,Wang, Yangli,Sun, Guochang.

[16]JMJ704 positively regulates rice defense response against Xanthomonas oryzae pv. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators. Hou, Yuxuan,Wang, Ling,Liu, Lianmeng,Li, Lu,Sun, Lei,Huang, Shiwen,Wang, Liyuan,Rao, Qiong,Zhang, Jian. 2015

[17]Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight. Luo, Yanchang,Sangha, Jatinder S.,Yin, Zhongchao,Luo, Yanchang,Wang, Shouhai,Li, Zefu,Yang, Jianbo.

[18]OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae. Wang, Nu,Wu, Maosen,Tian, Fang,Chen, Huamin,Yang, Fenghuan,He, Chenyang,Yuan, Xiaochen,Yang, Ching-Hong. 2016

[19]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[20]Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D, L-lactic acid) nanospheres. Zheng, Sisi,Luo, Li,Liu, Zhenguang,Bo, Ruonan,Hu, Yuanliang,Liu, Jiaguo,Wang, Deyun,Qin, Tao,Huang, Yifan,Wang, Deyun,Lu, Yu. 2018

作者其他论文 更多>>

微信小程序