Quantitative Determination of Selenium Species in Se-enriched Yeast Using Liquid Chromatography-Hydride Generation Atomic Fluorescence Spectrometry

文献类型： 外文期刊

第一作者： Xiao Zhi-Ming

作者： Xiao Zhi-Ming;Song Rong;Jia Zheng;Li Yang;Fan Xia

作者机构：

关键词： Liquid chromatography;Hydride generation atomic fluorescence spectrometry;Se-enriched yeast;Selenium species

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2014 年 42 卷 9 期

页码：

收录情况： SCI

摘要： A rapid and sensitive method has been developed for the simultaneous determination of four selenium species Se(VI), Se(IV), selenomethionine, and Se-methylselenocysteine in Se-enriched yeast by liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS). The isolation of the analytes from yeast samples was accomplished by protease XIV and trypsin enzymatic digestion. The target compounds were separated on a PRP-X100 anion exchange column and analyzed by HG-AFS. The mobile phase was 20 mmol/L (NH4)(2)HPO4. Good linearity was obtained for all the selenium species, with linear correlation coefficients higher than 0.9996. The LODs of the four species were between 0.5 and 5.0 mu g/kg. Average recoveries for the four analytes were in the ranges of 82.5%-101.2%, with intra-and inter-day RSD lower than 8.6% and 14.5, respectively. The proposed analytical method is simple, sensitive, with low operation cost, making it applicable for the determination of the selenium species in Se-enriched feeds.

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