Fate of florfenicol and linezolid resistance genes and their bacterial hosts during two waste treatment models in swine feedlots

文献类型： 外文期刊

第一作者： Wang, Zheng

作者： Wang, Zheng;Zheng, Yong-Liang;Fu, Yulin;Jiang, Haiyang;Wu, Congming;Lv, Ziquan;Schwarz, Stefan;Wang, Yang;Jiang, Nansong;Krueger-Haker, Henrike;Fessler, Andrea T.;Schwarz, Stefan;Krueger-Haker, Henrike;Fessler, Andrea T.;Schwarz, Stefan;Fu, Yulin

作者机构：

关键词： Florfenicol and linezolid cross-resistance genes; Excreta from swine farms; Waste treatment processes; Mobile genetic elements

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：8.2； 五年影响因子：8.6 ）

ISSN： 0048-9697

年卷期： 2024 年 939 卷

页码：

收录情况： SCI

摘要： Florfenicol resistance genes (FRGs) are widely present in livestock farms. The aim of this study was to evaluate the removal efficiencies of FRGs as well as the relationships between FRGs, mobile genetic elements (MGEs) and bacterial communities during the natural drying (ND) and anaerobic digestion (AD) processes of manure treatment in swine farms by combining bacterial isolation, quantitative PCR and metagenomic approaches. Solid manure showed a higher abundance of FRGs than fresh manure and was the main contamination source of fexA and fexB in ND farms, whilst biogas slurry displayed a lower abundance of FRGs than the wastewater in AD farms. Moreover, fresh manure and wastewater showed a high abundance of optrA , and wastewater was the main contamination source of cfr in both ND and AD farms. Both optrA / fexA -positive enterococci and cfr / fexA -positive staphylococci were mainly isolated along the farms ' treatment processes. The cfr -positive staphylococci were highly prevalent in wastewater (57.14 % - 100 %) and may be associated with nasal -derived cfr -positive porcine staphylococci. An increased abundance of Enterococcus , Jeotgalibaca and Vagococcus in the bacterial community structures may account for the high optrA abundance in wastewater and Jeotgalibaca may be another potential host of optrA . Furthermore, the abundance of FRG -related MGEs increased by 22.63 % after the ND process and decreased by 66.96 % in AD farms. A significant correlation was observed between cfr and IS Enfa4 , whereas no significance was found between optrA and IS 1216E , although IS 1216E is the predominant insertion sequence involved in the transfer of optrA . In conclusion, manure and wastewater represented independent pollution sources of FRGs in swine farms. Associated MGEs might play a key role in the transfer and persistence of FRGs. The AD process was more efficient in the removal of FRGs than the ND method, nevertheless a longer storage of slurry may be required for a complete removal.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序