Improvement of the propamocarb-tolerance of Lecanicillium lecanii through UV-light radiation-based mutagenesis

文献类型： 外文期刊

第一作者： Xie, Ming

作者： Xie, Ming;Li, Qian;Hu, Xin-Ping;Zhang, Yan-Jun;Peng, De-Liang;Zhang, Zhao-Rong;Li, Qiang;Liu, Zhi-Qi

作者机构：

关键词： Biological control;Entomopathogenic fungi;Fungicide-tolerance;Ultraviolet radiation

期刊名称：CROP PROTECTION （ 影响因子：2.571； 五年影响因子：3.11 ）

ISSN： 0261-2194

年卷期： 2018 年 103 卷

页码：

收录情况： SCI

摘要： Lecanicillium lecanii is an important entomopathogenic fungus which is used as a biopesticide for control of sucking insects. However, low fungicide-tolerance limits its application in the field. To improve the propamocarb-tolerance of L. lecanii, an optimized UV-light radiation-based mutagenesis system was established. The radiation time was optimized to 60 s for L. lecanii in the first round of UV-light radiation when a 20 Watt germicidal lamp was placed at a distance of 45 cm from plates. Both the positive mutation rate and EC50 value of the UV-mutant were the highest when using this radiation time. The tolerance ratio (TR) was always lower than 1.13 in the first round of UV-light radiation. To further increase fungal propamocarb-tolerance, the highest propamocarb-tolerant mutant in the first round of UV-light radiation was used as a starting strain for multiple rounds of UV-light radiation in which a same germicidal lamp and radiation parameter were applied as described in the first round of UV-light radiation. TR was largely accumulated to 2.13 on the fourth round of radiation, and then began to level off and remained constant. A higher propamocarb-tolerant mutant was obtained in the sixth-round UV-light radiation, whose median effective concentration (EC50) value was increased 2.15-fold compared to the wild-type strain, and it was mitotically stable in 20 passages on PDAY medium. This mutant did not show any significant changes of conidial yield on plates and virulence on aphids, although its colony growth was significantly lower than that of the wild-type strain. After the application of propamocarb at a recommended field dose of 550 mu g/ml, the interval time of using L. lecanii was significantly shortened in this mutant compared to the wild-type strain. The results indicated that this improved strain could be potentially applied in combination with propamocarb in the field. (C) 2017 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Effects of a NTG-based chemical mutagenesis on the propamocarb-tolerance of the nematophagous fungus Lecanicillium attenuatum. Li, Qian,Hu, Xin-Ping,Zhang, Yan-Jun,Peng, De-Liang,Zhang, Xiao-Lin. 2017

[2]Entomopathogenicity of Three Muscardine Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae, against the Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). Ma, Chun-Sen,Majeed, M. Z.,Fiaz, M.,Afzal, M.. 2017

[3]Laboratory evaluation of virulence of Chinese Beauveria bassiana and Metarhizium anisopliae isolates to engorged female Rhipicephalus (Boophilus) microplus ticks. Ren, Qiaoyun,Liu, Zhijie,Guan, Guiquan,Sun, Ming,Ma, Miling,Niu, Qingli,Li, Youquan,Liu, Aihong,Liu, Junlong,Yang, Jifei,Yin, Hong,Luo, Jianxun. 2012

[4]Susceptibility of the tick Haemaphysalis qinghaiensis to isolates of the fungus Metarhizium anisopliae in China. Ren, Qiaoyun,Sun, Ming,Guan, Guiquan,Liu, Zhijie,Chen, Ze,Liu, Aihong,Li, Youquan,Ma, Miling,Yang, Jifei,Niu, Qingli,Liu, Junlong,Yin, Hong,Luo, Jianxun,Han, Xueqing.

[5]Laboratory evaluation of Beauveria bassiana and Metarhizium anisopliae in the control of Haemaphysalis qinghaiensis in China. Ren, Qiaoyun,Chen, Ze,Luo, Jin,Liu, Guangyuan,Guan, Guiquan,Liu, Zhijie,Liu, Aihong,Li, Youquan,Niu, Qingli,Liu, Junlong,Yang, Jifei,Yin, Hong,Luo, Jianxun,Yin, Hong,Han, Xueqing.

[6]Mutagenic effects of low-energy N+ ion implantation on the propamocarb-tolerance of nematophagous fungus Lecanicillium attenuatum. Xie, Ming,Zhang, Xiao-Lin,Hu, Xin-Ping,Zhang, Yan-Jun,Peng, De-Liang,Li, Qian,Li, Ming. 2018

[7]Response of oxidative stress defense systems in rice (Oryza sativa) leaves with supplemental UV-B radiation. Dai, QJ,Yan, B,Huang, SB,Liu, XZ,Peng, SB,Miranda, MLL,Chavez, AQ,Vergara, BS,Olszyk, DM. 1997

[8]Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system. Khan, Sehroon,Xu, Jianchu,Khan, Sehroon,Xu, Jianchu,Khan, Sehroon,Guo Lihua,Qiu Dewen,Nadir, Sadia,Nadir, Sadia,Holmes, Keith A..

[9]Further notes on the molecular taxonomy of Metarhizium. Huang, B,Humber, RA,Li, SG,Li, ZZ,Hodge, KT. 2005

[10]Establishment of polyethylene-glycol-mediated protoplast transformation for Lecanicillium lecanii and development of virulence-enhanced strains against Aphis gossypii. Xie, Ming,Zhang, Xiao-Lin,Peng, De-Liang,Yu, Wen-Bin,Li, Qian,Zhao, Jin-Jin,Zhang, Zhao-Rong,Li, Qiang,Li, Qian.

[11]Metarhizium anisopliae infection alters feeding and trophallactic behavior in the ant Solenopsis invicta. Qiu, Hua-Long,He, Yu-Rong,Lu, Li-Hua,Zalucki, M. P..

[12]Effect of parasitism on flight behavior of the soybean aphid, Aphis glycines. Wu, Kongming,Wyckhuys, Kris A. G.,Heimpel, George E..

[13]Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods. Liu, Xingzhong,Gao, Li.

[14]Diversity of Endophytic Fungi from Different Verticillium-Wilt-Resistant Gossypium hirsutum and Evaluation of Antifungal Activity Against Verticillium dahliae In Vitro. Li, Zhi-Fang,Wang, Ling-Fei,Feng, Zi-Li,Zhao, Li-Hong,Shi, Yong-Qiang,Zhu, He-Qin.

[15]Artificial diet development and its effect on the reproductive performances of Propylea japonica and Harmonia axyridis. Ali Intazar,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min Lv,JinJie Cui. 2016

[16]Influence of five aphid species on development and reproduction of Propylaea japonica (Coleoptera: Coccinellidae). Zhang, Shi-Ze,Li, Jian-Jun,Shan, Hong-Wei,Liu, Tong-Xian,Zhang, Fan. 2012

[17]Suitability of various prey types for the development of Propylea japonica (Coleoptera : Coccinellidae). Zhang, Shi-Ze,Zhang, Fan,Hua, Bao-Zhen. 2007

[18]Diversity and biocontrol potential of endophytic fungi in Brassica napus. Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Qinghua,Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Lei,Chen, Weidong. 2014

[19]Interspecific competition between Peristenus spretus and Peristenus relictus (Hymenoptera: Braconidae), larval parasitoids of Apolygus lucorum (Hemiptera: Miridae). Wu, Kongming,Luo, Shuping,Zhang, Feng,Wu, Kongming,Zhang, Feng. 2018

[20]Ecology, Worldwide Spread, and Management of the Invasive South American Tomato Pinworm, Tuta absoluta: Past, Present, and Future. Guedes, Raul Narciso C.,Wan, Fang-Hao,Desneux, Nicolas. 2018

作者其他论文 更多>>

微信小程序